Journal: Scientific Reports

Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

doi: 10.1038/srep42892

Figure Lengend Snippet: Nucleotide mismatches in each genus are highlighted with orange colored box. ( a ) Orthopoxviruses show T:G mismatch between CPXV and CMLV. ( b ) AAT:AGT:GGC nucleotide variations exist for GTPV, SPPV and LSDV of capripoxviruses. ( c ) Parapoxviruses show TTAT:CTAG:CACG nucleotide mismatches for ORFV, PCPV and BPSV, respectively. The forward and reverse primer sequences are underlined flanking the nucleotide variations. Identical nucleotides are shown as dots in reference to the first sequence.

Article Snippet: Capripoxviruses (GTPV-Denizli, SPPV-Denizli and LSDV-Ismalia), orthopoxviruses (CMLV- Hadow/01/2012 and CPXV-72/93), and parapoxviruses (ORFV- DZ C-1, PCPV-2200/12 and BPSV- Stamm M1) were used for the production of positive control plasmids ( ).

Techniques: Sequencing

Journal: Scientific Reports

Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

doi: 10.1038/srep42892

Figure Lengend Snippet: Three primer pairs were used for the amplification. Each virus genotype clustered separately within the genus. The normalized melt curve and difference curve plots are presented separately with different line colour for each genotype within the genus: for the eight poxviruses ( a , b ), orthopoxviruses ( c , d ), capripoxviruses ( e , f ), and parapoxviruses ( g , h ), respectively. Green and red columns in the normalized melt curve plot represent pre- and post-melt normalization regions.

Article Snippet: Capripoxviruses (GTPV-Denizli, SPPV-Denizli and LSDV-Ismalia), orthopoxviruses (CMLV- Hadow/01/2012 and CPXV-72/93), and parapoxviruses (ORFV- DZ C-1, PCPV-2200/12 and BPSV- Stamm M1) were used for the production of positive control plasmids ( ).

Techniques: Amplification, Virus