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Image Search Results
Journal: PLoS Neglected Tropical Diseases
Article Title: Localized skin inflammation during cutaneous leishmaniasis drives a chronic, systemic IFN-γ signature
doi: 10.1371/journal.pntd.0009321
Figure Lengend Snippet: (A) Principal component analysis showing principal component 1 (PC1) and PC2 for RNA-seq data from the peripheral blood of CL patients (yellow triangles, n = 51) and HS (blue circles, n = 14). A PERMANOVA statistical test was used to calculate distances between the groups, Pr(>F) = .006. (B) Gene set enrichment analysis (GSEA) showing normalized enrichment scores (NES) of the top 10 pathways from the Reactome Pathway Database enriched in the peripheral blood of CL patients relative to HS. (C) Volcano plot highlighting overrepresented genes (n = 51) in the peripheral blood from CL patients relative to healthy subjects, FDR≤0.01 and logFC≥1. The Interferome database was used to identify Interferon-stimulated genes (ISGs), represented in red circles (n = 42, 82%). Other genes are colored in blue (n = 9, 18%).
Article Snippet: Gene Set Enrichment Analysis (GSEA) was carried out using
Techniques: RNA Sequencing
Journal: PLoS Neglected Tropical Diseases
Article Title: Localized skin inflammation during cutaneous leishmaniasis drives a chronic, systemic IFN-γ signature
doi: 10.1371/journal.pntd.0009321
Figure Lengend Snippet: (A) Expression of genes encoding cytolytic granules ( GZMA , GZMB , GZMH , GNLY , and PRF1 ) in the peripheral blood of HS and CL patients. Wilcoxon rank-sum test was used for statistical analysis, *P<0.05, **P<0.01, ***P<0.001 and ****P < .0001. Gene expression is represented as counts per million (CPM) in log2 scale. (B) GSEA enrichment plots showing three cytotoxicity-related pathways from the Biocarta and KEGG Pathway Databases enriched in the CL peripheral blood relative to HS. Pathway name is indicated in parenthesis, next to the pathway database source. Light blue box indicates genes included in the Leading Edge subgroup from each pathway that were enriched and differentially expressed in CL relative to HS. Genes are shown in ranked order according to their running enrichment score. NES, normalized enrichment score; FDR, false discovery rate.
Article Snippet: Gene Set Enrichment Analysis (GSEA) was carried out using
Techniques: Expressing, Gene Expression