Journal: Biological Procedures Online

Article Title: Machine learning-driven multi-omics analysis identifies a prognostic gene signature associated with programmed cell death and metabolism in hepatocellular carcinoma

doi: 10.1186/s12575-025-00286-1

Figure Lengend Snippet: Pathological and molecular analysis of CCT3 expression in the Akt/Nras and c-Myc HCC models. A Hematoxylin-eosin (H&E) staining showing characteristic pathological features of HCC in the Akt/Nras and c-Myc models. B - C Immunohistochemistry and Western blot analysis demonstrate significantly upregulated CCT3 expression in the Akt/Nras and c-Myc models compared to control groups. D Spatial localization of CCT3 is predominantly in tumor regions within HCC tissues, with minimal expression in immune or stromal cells. E Pathway analysis reveals a positive correlation between CCT3 and the cell cycle pathway. F siRNA-mediated knockdown of CCT3 in SNU449 cells leads to a significant reduction in Cyclin B1 and PCNA expression. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The HCC lines HLF and Huh-7 were sourced from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and the SNU449 cells were obtained from Procell Life Science and Technology Co., Ltd. THLE-2 cells were cultured in a specialized medium as recommended by the supplier.

Techniques: Expressing, Staining, Immunohistochemistry, Western Blot, Control, Knockdown

Journal: Biological Procedures Online

Article Title: Machine learning-driven multi-omics analysis identifies a prognostic gene signature associated with programmed cell death and metabolism in hepatocellular carcinoma

doi: 10.1186/s12575-025-00286-1

Figure Lengend Snippet: Effects of CCT3 knockdown on HCC cell viability, clonogenicity, migration, and molecular targets. A Western blot experiment showing CCT3 knockdown in SNU449 cells. B A cell viability assay was performed to assess the effects of siRNA-mediated CCT3 knockdown on SNU449 cells. C Clonogenicity and Migration assays were conducted to evaluate the clonogenic potential of SNU449 cells after CCT3 silencing. D Western blot analysis was performed to assess FTH1, GPX4, and LC3 expression levels after CCT3 silencing. * P < 0.05; ** P < 0.01; **** P < 0.0001

Article Snippet: The HCC lines HLF and Huh-7 were sourced from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and the SNU449 cells were obtained from Procell Life Science and Technology Co., Ltd. THLE-2 cells were cultured in a specialized medium as recommended by the supplier.

Techniques: Knockdown, Migration, Western Blot, Viability Assay, Expressing