Journal: Scientific reports

Article Title: PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib.

doi: 10.1038/s41598-022-16623-3

Figure Lengend Snippet: Figure 2. Cell line-dependent reversibility of acquired Talazoparib resistance. (a) Immunoblot showing PAR level of PSN1 parental cells, TalaR cells maintained in media with Talazoparib (TalaR-M) and TalaR cells cultured in drug-free media for 4 weeks (TalaR-DF). (b) Talazoparib IC50 chart showing reversibility of acquired resistance in PSN1 cells over 4 weeks after drug withdrawal. (c–f) Clonogenic assay showing Talazoparib sensitivity of parental and acquired resistant cells in PSN1, PANC1, SW1990 and HCC1806 cells which were maintained in drug media (Tala-M) and in drug free media for 4 weeks (Tala-DF). (g, h) Volcano plots showing differentially expressed genes between TalaR-M and TalaR-DF in PSN1 and HCC1806 cells. (i, j) UMAP representations of HCC1806 parental and resistant subpools, colored by cell line (i) or cluster identity (j). (k, l) Violin plots of SNAI2 (k) and TWIST1 (l) gene expression profile across subpopulation clusters shown in (j).

Article Snippet: SNAI2 cDNA expressing plasmid was purchased from Origene (RC202365) and transfected into cells by using Fugene HD transfection reagent (Promega).

Techniques: Western Blot, Cell Culture, Clonogenic Assay, Gene Expression

Journal: Scientific reports

Article Title: PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib.

doi: 10.1038/s41598-022-16623-3

Figure Lengend Snippet: Figure 5. CHD1L depletion re-sensitizes cells with acquired resistance to Talazoparib. (a) Clonogenic assay showing growth of PSN1 TalaR-M cells with CHD1L stable knockdown. (b) Immunoblot showing expression level of CHD1L and SNAI2 in CHD1L stable knockdown cells as in (a). (c) IncuCyte curves showing the growth PSN1 TalaR-M with inducible CHD1L knockdown with and without doxycycline induction. (d) qPCR showing mRNA level of CHD1L and SNAI2 in PSN1 TalaR-M with inducible CHD1L knockdown with and without doxycycline induction as in (c). (e) IncuCyte curves showing the growth of PSN1 parental and TalaR-M with CHD1L knockout. (f) IncuCyte curves showing the growth of HCC1806 parental and TalaR-M with CHD1L knockout. (g, h) Immunoblot and qPCR showing CHD1L and SNAI2 level in HCC1806 parental and TalaR-M with CHD1L knockout as in (f).

Article Snippet: SNAI2 cDNA expressing plasmid was purchased from Origene (RC202365) and transfected into cells by using Fugene HD transfection reagent (Promega).

Techniques: Clonogenic Assay, Knockdown, Western Blot, Expressing, Knock-Out

Journal: Developmental biology

Article Title: Draxin alters laminin organization during basement membrane remodeling to control cranial neural crest EMT

doi: 10.1016/j.ydbio.2018.12.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: We thank Dr. M. Simoes-Costa for providing the NC1.1 M3:EGFP construct, and Dr. M. Cheung for providing the pCIG-V5-Snail2-IRES-nls-GFP construct (Addgene plasmid # 44282).

Techniques: Recombinant, Control, Plasmid Preparation, Software

Journal: Scientific Reports

Article Title: TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells

doi: 10.1038/srep23775

Figure Lengend Snippet: All pairs of F/R primers used for real-time qPCR.

Article Snippet: Rabbit polyclonal antibodies against MMP9, SNAI1, p16 and Vimentin were bought from BioSynthesis (Beijing, China), whilst other rabbit antibodies against α-Catenin, CDK2, CDK6, Cyclin D1, SNAI2, FN1, Rb1, p53 and p21 were purchased from BosTer (Wuhan, China).

Techniques:

Journal: Cells

Article Title: Cholest-4,6-Dien-3-One Promote Epithelial-To-Mesenchymal Transition (EMT) in Biliary Tree Stem/Progenitor Cell Cultures In Vitro

doi: 10.3390/cells8111443

Figure Lengend Snippet: Cholest-4,6-dien-3-one enhanced the EMT markers analyzed by RT-qPCR. Relative gene expression of EMT markers ( TWIST1 , TWIST2 , SNAIL1 , SNAIL2 , ZEB1 , and ZEB2 ) examined by RT-qPCR of hBTSCs cultured in basal condition (KM), KM supplemented with cholest-4,6-dien-3-one, differentiation tailored cholangiocyte medium (CM) or CM added with cholest-4,6-dien-3-one. Data expressed as mean ± SD of N = 6 experiments; ¤ p < 0.0001.

Article Snippet: Twist 1 (Cusabio, Houston, Texas, USA #CSB-EL025358HU), Twist2 (Mybiosurce, San Diego, California, USA #MBS773864), Snail1 (LSBio, Seattle, Washington, USA #LS-F2317), Snail2 (Biomatik, Cambridge, Ontario, Canada #EKC35582), Zeb1 (Biomatik, Cambridge, Ontario, Canada #EKC36072), and Zeb2 (LSBio, Seattle, Washington, USA #LS-F13506) were measured by human-specific ELISA kit according to protocols provided by companies.

Techniques: Quantitative RT-PCR, Gene Expression, Cell Culture

Journal: Cells

Article Title: Cholest-4,6-Dien-3-One Promote Epithelial-To-Mesenchymal Transition (EMT) in Biliary Tree Stem/Progenitor Cell Cultures In Vitro

doi: 10.3390/cells8111443

Figure Lengend Snippet: Cholest-4,6-dien-3-one enhanced the EMT markers analyzed by WB. Protein levels of EMT markers (TWIST1, TWIST2, SNAIL1, SNAIL2, ZEB1, and ZEB2) examined by WB of hBTSCs cultured in basal condition (KM), KM supplemented with cholest-4,6-dien-3-one, differentiation tailored cholangiocyte medium (CM) or CM added with cholest-4,6-dien-3-one. Data expressed as mean ± SD of N = 6 experiments; ¤ p < 0.0001.

Article Snippet: Twist 1 (Cusabio, Houston, Texas, USA #CSB-EL025358HU), Twist2 (Mybiosurce, San Diego, California, USA #MBS773864), Snail1 (LSBio, Seattle, Washington, USA #LS-F2317), Snail2 (Biomatik, Cambridge, Ontario, Canada #EKC35582), Zeb1 (Biomatik, Cambridge, Ontario, Canada #EKC36072), and Zeb2 (LSBio, Seattle, Washington, USA #LS-F13506) were measured by human-specific ELISA kit according to protocols provided by companies.

Techniques: Cell Culture

Journal: Cells

Article Title: Cholest-4,6-Dien-3-One Promote Epithelial-To-Mesenchymal Transition (EMT) in Biliary Tree Stem/Progenitor Cell Cultures In Vitro

doi: 10.3390/cells8111443

Figure Lengend Snippet: BMP-4 and SHH protein activation pathways induced the expression of EMT markers. Relative gene expression of EMT markers TWIST1 , TWIST2 , SNAIL1 , SNAIL2 , ZEB1 , and ZEB2 ) measured by RT-qPCR of hBTSCs cultured in basal condition (KM), BMP-M or SHH-M. Data expressed as mean ± SD of N = 6 experiments; * p < 0.05; § p < 0.01.

Article Snippet: Twist 1 (Cusabio, Houston, Texas, USA #CSB-EL025358HU), Twist2 (Mybiosurce, San Diego, California, USA #MBS773864), Snail1 (LSBio, Seattle, Washington, USA #LS-F2317), Snail2 (Biomatik, Cambridge, Ontario, Canada #EKC35582), Zeb1 (Biomatik, Cambridge, Ontario, Canada #EKC36072), and Zeb2 (LSBio, Seattle, Washington, USA #LS-F13506) were measured by human-specific ELISA kit according to protocols provided by companies.

Techniques: Activation Assay, Expressing, Gene Expression, Quantitative RT-PCR, Cell Culture

Journal: Journal of Ovarian Research

Article Title: Survival and biomarker analysis for ovarian mucinous carcinoma according to invasive patterns: retrospective analysis and review literature

doi: 10.1186/s13048-021-00783-3

Figure Lengend Snippet: Representative images of positive cells of each immunohistochemistry a Cytokeratin 7 (x200); b Cytokeratin 20 (x200); c Caudal type homeobox 2 (x200); d Human epidermal growth factor receptor 2 (x200); e Cytokeratin 5/6 (x200); f Androgen receptor (x200); g Aldehyde dehydrogenase 1 (x200); h Cluster of differentiation 24 (x200); i Cluster of differentiation 133 (x200); j Programmed cell death 1 (x200); k Programmed cell death 1- ligand 1 (x200); l Zinc finger E-box binding homeobox 1 (x200); m Met (x200); n Epithelial growth factor receptor (x200); o Snail-2 (x200)

Article Snippet: Snail-2 , Polyclonal (Rabbit) , PAB1923 , Abnova , x200 , Nucleus , MCF, MDA-MB-231 , Citrate , More than 10 % positive cell with staining intensity score 1 or 3 was defined as positive..

Techniques: Immunohistochemistry, Binding Assay

Journal: Cancer Science

Article Title: Pathological features of triple‐negative breast cancers that showed progressive disease during neoadjuvant chemotherapy

doi: 10.1111/cas.13274

Figure Lengend Snippet: Antibodies used for immunohistochemistry in the present study

Article Snippet: Snail‐2 , Poly (R) , PAB1923 , Abnova , ×200 , N , MCF7, MDA‐MB‐231.

Techniques: Immunohistochemistry, Control