Journal: Journal of Tissue Engineering

Article Title: Outgrowing endothelial and smooth muscle cells for tissue engineering approaches

doi: 10.1177/2041731417698852

Figure Lengend Snippet: This figure shows representative immunofluorescence staining and FACS analysis of outgrown cells cultivated with (a) EGM-2 and (b) SGM-2. In both settings, most cells expressed CD34 (red, upper row in both columns), whose portion decreased slightly during further cultivation. During cultivation with EGM-2, colony-forming cells positive for CD31 (green, middle row) and NOS (red, bottom both in the left column) became the predominant cell type. During cultivation with SGM-2, colony-forming LOSMC positive for sm-alpha-actin-positive (green, middle row) and about 57% positive for smoothelin (red, bottom row) were the predominant cell type. Compare for antigen expression.

Article Snippet: Smoothelin (Acris antibodies).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Journal of Tissue Engineering

Article Title: Outgrowing endothelial and smooth muscle cells for tissue engineering approaches

doi: 10.1177/2041731417698852

Figure Lengend Snippet: Antigen expression (expressed as percentage of positive cells by the total number of cells).

Article Snippet: Smoothelin (Acris antibodies).

Techniques: Expressing

Journal: Science Advances

Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix

doi: 10.1126/sciadv.adr3530

Figure Lengend Snippet: ( A ) To visualize SMCs, transverse sections of whole cervices from days 12, 15, 17, and 19 gravid mice ( n ≥ 3) were immunofluoresently stained with specific markers for a contractile (MYH11 and SMTN) or synthetic phenotype (RBP1 and MYH10). Sections were also stained with CNN1 and α-SMA, which are markers of contractile smooth muscle and myofibroblasts. The grayscale bar (left) is a schematic representation of markers associated with a particular SMC phenotype. Green indicates positive immunostaining for each marker; blue indicates nuclei counterstained with 4′,6-diamidino-2-phenylindole (DAPI). ( B ) Control immunoglobulin G (IgG; left) was used as a control for the primary antibodies, and primary antibodies were omitted as controls for the secondary antibodies. Representative dual immunostaining of MYH10 (green) and MYH11 (red). Scale bars, 1000 μm; inset scale bars, 60 μm. Inset depicts high-power view of select regions (dashed box) of interest, which show longitudinal- and circumferentially oriented contractile SMCs, as well as synthetic SMCs in the broad subepithelial region and diffusely throughout the stroma.

Article Snippet: Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α-SMA (1:200 dilution; Cell Signaling Technologies, 19245), or smoothelin (1:100; Novus Biologicals, NBP2-37931).

Techniques: Staining, Immunostaining, Marker, Control

Journal: European journal of medical research

Article Title: Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro.

doi: 10.1186/s40001-024-01843-2

Figure Lengend Snippet: Fig. 1 Phenotypic identification of BVSMCs (A–I). A-C α-SMA fluorescence staining of BVSMCs, α-SMA: green; nucleus: blue; C is the merged figure of A and B; D–F calponin fluorescence staining, calponin: red; nucleus: blue; F is the merged figure of D and E; G–I Smoothelin fluorescence staining, smoothelin: green; nucleus: blue; I is the merged figure of G and H; scale bar: 100 μm

Article Snippet: Antibodies used were α-SMA (1:200, NBP1-30,894, Novus Biologicals, USA), calponin (1:200, 13,938–1-AP, Proteintech, USA), and smoothelin (1:200, orb158429, Biorbyt, UK).

Techniques: Fluorescence, Staining

Journal: Endocrinology

Article Title: Prenatal Exposures of Male Rats to the Environmental Chemicals Bisphenol A and Di(2-Ethylhexyl) Phthalate Impact the Sexual Differentiation Process.

doi: 10.1210/en.2015-1077

Figure Lengend Snippet: Figure 6. Male rats were exposed to bisphenol A (BPA) and di (2-ethylhexyl) phthalate (DEHP) by maternal gavage from gestational days 12–21.Testis from adult male rats at 90 days of age (n 3) were processed to obtain gDNA and measure 5-methyl cytosine (5mc) and 5- hydroxymethyl cytosine (5hmc) concentrations by liquid chromatography electrospray ionization tandem mass spectrometry with single reaction monitoring (A, B, E, F). Testicular tissue (n 6) was analyzed for Dnmt3a and Dnmt3b protein expression using Western blotting procedures and antisera specific to rat Dnmt3a and Dnmt3b (C, D, G, H). *P .05 vs control.

Article Snippet: Dnmt3a (C-12) Santa Cruz Biotechnologies, Sc-365 769 Mouse monoclonal 0.0002 MW ( Dnmt3a) 100–130 kDa IgG Dnmt3b Belongs to C5-methyltransferase family.

Techniques: Liquid Chromatography, Mass Spectrometry, Expressing, Western Blot, Control