Journal: BMC Genomics

Article Title: A comparison between whole transcript and 3’ RNA sequencing methods using Kapa and Lexogen library preparation methods

doi: 10.1186/s12864-018-5393-3

Figure Lengend Snippet: RT-qPCR results

Article Snippet: We used the KAPA Stranded mRNA-Seq Kit (Trad-KAPA) to prepare libraries using the whole transcript method, and the Lexogen QuantSeq 3’mRNA-Seq Library Prep Kit-FWD (3’-LEXO) to prepare 3′ libraries.

Techniques:

Journal: BMC Genomics

Article Title: A comparison between whole transcript and 3’ RNA sequencing methods using Kapa and Lexogen library preparation methods

doi: 10.1186/s12864-018-5393-3

Figure Lengend Snippet: The number of differentially expressed transcripts detected by the Trad-KAPA and 3’-LEXO, before and after subsampling from 10 million reads

Article Snippet: We used the KAPA Stranded mRNA-Seq Kit (Trad-KAPA) to prepare libraries using the whole transcript method, and the Lexogen QuantSeq 3’mRNA-Seq Library Prep Kit-FWD (3’-LEXO) to prepare 3′ libraries.

Techniques:

Journal: Cell host & microbe

Article Title: Epitranscriptomic addition of m 5 C to HIV-1 transcripts regulates viral gene expression

doi: 10.1016/j.chom.2019.07.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The gRNA from HIV-1 infected CEM was processed using the SMARTer® Stranded Total RNA-Seq Kit v2 following the manufacturer’s protocol.

Techniques: Virus, Recombinant, Plasmid Preparation, Sample Prep, SYBR Green Assay, Gene Expression, Magnetic Beads, Luciferase, Enzyme-linked Immunosorbent Assay, Software, SDS Page

Journal: Cancer cell

Article Title: Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapy

doi: 10.1016/j.ccell.2022.10.001

Figure Lengend Snippet:

Article Snippet: The isolated RNA for each of the sorted CD3 + CD4 + , CD3 + CD8 + , CD3 − CD11b + , and CD3 − CD11b − CD19 + cells was sent to iTeos, Inc., for bulk RNA-seq library preparation with a SMART-Seq v4 ultra low input mRNA sample prep kit and 100-bp paired end sequencing by Eurofins.

Techniques: Multiplex Assay, Immunohistochemistry, Recombinant, Staining, Plasmid Preparation, RNA HS Assay, Sequencing, Software, Cytometry