Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: Summary of Nanoparticles for Stroke Treatment by Angiogenesis

Article Snippet: Small extracellular vesicles secreted by mesenchymal stem cells (MSC) derived from human iPSC (iMSC-sEV) , Biomimetic nanoparticles , iMSC-sEV , Ischemic site , IV , Angiogenesis is a major obstacle to full recovery after a stroke, and iMSC-sEV, or small extracellular vesicles, released by MSCs generated from human iPSC, show tremendous promise in this regard. Treatment with iMSC-sEV prevented ischemia damage and increased angiogenesis, likely by preventing autophagy , After administration of iMSC-sEV, the number of CD31 + cells in the infarcted brain area further increased, which could promote angiogenesis , [ ] .

Techniques: Over Expression, Formulation, In Vitro, Activity Assay, Molecular Weight, Microinjection, Functional Assay, shRNA, Plasmid Preparation, Expressing, Adsorption, Permeability, Comparison, Cell Culture, Membrane, Disruption, Preserving, Liposomes, Derivative Assay, Generated, In Vivo, Control, Knock-Out, Injection

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: iMSC-sEV promote angiogenesis following stroke and increase HUVEC migration and tube formation after OGD. ( A – D ) Seven days after MCAO, angiogenesis was evaluated by immunofluorescence staining of CD31/EdU and CD34. Scale bar = 100 μm. * P < 0.05. Reproduced from Xia Y, Ling X, Hu G, et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther . 2020;11(1):313. Creative Commons.

Article Snippet: Small extracellular vesicles secreted by mesenchymal stem cells (MSC) derived from human iPSC (iMSC-sEV) , Biomimetic nanoparticles , iMSC-sEV , Ischemic site , IV , Angiogenesis is a major obstacle to full recovery after a stroke, and iMSC-sEV, or small extracellular vesicles, released by MSCs generated from human iPSC, show tremendous promise in this regard. Treatment with iMSC-sEV prevented ischemia damage and increased angiogenesis, likely by preventing autophagy , After administration of iMSC-sEV, the number of CD31 + cells in the infarcted brain area further increased, which could promote angiogenesis , [ ] .

Techniques: Migration, Immunofluorescence, Staining, Derivative Assay

Journal: Scientific Reports

Article Title: Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device

doi: 10.1038/s41598-023-45409-4

Figure Lengend Snippet: Device layout and graphical outline of experimental flow. Small extracellular vesicles (sEVs) were simultaneously extracted from human biofluids using the insulator-based dielectrophoretic (iDEP) device, wherein a trapping zone was created at the tip region of micropipettes by balancing the dielectrophoretic (DEP) force with two electrokinetic forces, including electroosmosis (EOF) and electrophoresis (EP). sEVs isolates were comprehensively characterized using flow cytometry, ImageStream x , and microRNA (miRNA) sequencing.

Article Snippet: Small extracellular vesicles (sEVs) are membrane-encapsulated biological vessels ranging from 40–150 nm in diameter. sEVs are released by cells into the extracellular space and can be found in essentially all biofluids including blood , , urine , saliva , human semen , breast milk , and cerebrospinal fluids .

Techniques: Electrophoresis, Flow Cytometry, Sequencing

Journal: Scientific Reports

Article Title: Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device

doi: 10.1038/s41598-023-45409-4

Figure Lengend Snippet: ( A ) FSC-H versus SSC-H of PS-COOH beads analysis on high-resolution flow cytometry represents the resolution of beads size in PS-COOH mix. ( B ) Histogram illustrating different sizes of PS-COOH beads and serum-derived small extracellular vesicles (sEVs). ( C ) Representative plots of PS-COOH-100 nm beads fluorescence-FITC. ( D ) Representative FSC-H versus SSC-H of sEVs isolates from human plasma. ( E ) Representative FSC-H versus SSC-H of unstained sEVs demonstrating gating. ( F ) Representative plots as negative controls i. Isotype-stained sEVs. ii. CD63 antibody in PBS and iii. CD81 antibody in PBS. ( G ) Representative dot plots of sEVs stained for CD63 from i. serum ii. plasma and iii. urine. ( H ) Representative plots of sEVs stained for CD81 from i. serum ii. plasma and iii. urine.

Article Snippet: Small extracellular vesicles (sEVs) are membrane-encapsulated biological vessels ranging from 40–150 nm in diameter. sEVs are released by cells into the extracellular space and can be found in essentially all biofluids including blood , , urine , saliva , human semen , breast milk , and cerebrospinal fluids .

Techniques: Flow Cytometry, Derivative Assay, Fluorescence, Clinical Proteomics, Staining

Journal: Scientific Reports

Article Title: Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device

doi: 10.1038/s41598-023-45409-4

Figure Lengend Snippet: ( A – C ) Size versus SSC of beads and calibration speed beads. ( D ) Size versus FITC illustrating the location on side scatter that is positive for fluorescent beads. ( E ) Representative dot plot of FITC vs. Scatter Intensity (top) and APC vs. Scatter Intensity (bottom). ( F ) Representative size vs. SSC plot of i. small extracellular vesicles (sEVs) purified from biofluid and negative controls: ii. PBS iii. antibody CD63 iv. antibody CD81 and v. Isotype. ( G ) Representative histogram of CD63+ sEVs from i. serum, ii. plasma, iii. urine, and positive controls: iv. hTERT-immortalized mesenchymal stem cell (MSC) sEVs, and v. A549 non-small cell lung carcinoma (NSCLC)-derived sEVs. ( H ) Representative histogram of CD81+ sEVs from i. serum, ii. plasma, iii. urine, and positive controls iv. hTERT-immortalized MSC sEVs, and v. A549 NSCLC sEVs.

Article Snippet: Small extracellular vesicles (sEVs) are membrane-encapsulated biological vessels ranging from 40–150 nm in diameter. sEVs are released by cells into the extracellular space and can be found in essentially all biofluids including blood , , urine , saliva , human semen , breast milk , and cerebrospinal fluids .

Techniques: Purification, Clinical Proteomics, Derivative Assay

Journal: Scientific Reports

Article Title: Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device

doi: 10.1038/s41598-023-45409-4

Figure Lengend Snippet: Heatmap of microRNA transcripts isolated from serum, plasma and urine small extracellular vesicles (sEVs) from patients with genitourinary tract cancers. Columns represent individual samples. Legend shows type of biofluid and cancer site for each sample. Rows represent microRNA gene transcripts. Color bar scale represents miRNA enrichment. RStudio Desktop (version 2023.06.2 + 561, accessible at https://posit.co/download/rstudio-desktop/ ) was used for heatmap generation.

Article Snippet: Small extracellular vesicles (sEVs) are membrane-encapsulated biological vessels ranging from 40–150 nm in diameter. sEVs are released by cells into the extracellular space and can be found in essentially all biofluids including blood , , urine , saliva , human semen , breast milk , and cerebrospinal fluids .

Techniques: Isolation, Clinical Proteomics

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: Summary of Nanoparticles for Stroke Treatment by Angiogenesis

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Over Expression, Formulation, In Vitro, Activity Assay, Molecular Weight, Microinjection, Functional Assay, shRNA, Plasmid Preparation, Expressing, Adsorption, Permeability, Comparison, Cell Culture, Membrane, Disruption, Preserving, Liposomes, Derivative Assay, Generated, In Vivo, Control, Knock-Out, Injection

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: iMSC-sEV promote angiogenesis following stroke and increase HUVEC migration and tube formation after OGD. ( A – D ) Seven days after MCAO, angiogenesis was evaluated by immunofluorescence staining of CD31/EdU and CD34. Scale bar = 100 μm. * P < 0.05. Reproduced from Xia Y, Ling X, Hu G, et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther . 2020;11(1):313. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Migration, Immunofluorescence, Staining, Derivative Assay

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: When PMNs are absent in vivo, sEVs produced from hypoxic MSCs promote microvascular remodeling after ischemic stroke. ( A – C ) The microvascular network properties in the ischemic cerebral cortex were examined using 3D light sheet microscopy in mice that were subjected to 40 minutes of MCAO and then survived for 14 days. A, microvascular length density; B, branch point density; and C, mean branch length. ( D ) Magnified axial views of the striatum and cortex in an ischemic mouse brain showing the regions of interest for determining the properties of the microvascular network. Images of the ischemic cortex taken using maximum intensity projection ( E ) from each of the five groups. * p <0.05 compared with isotype/vehicle; # p <0.05 compared with isotype/sEV hypoxic . Scale bars=500 µm (in ( D )/100 µm (in ( E ). Reproduced from Gregorius J, Wang C, Stambouli O, et al. Small extracellular vesicles obtained from hypoxic mesenchymal stromal cells have unique characteristics that promote cerebral angiogenesis, brain remodeling and neurological recovery after focal cerebral ischemia in mice. Basic Res Cardiol . 2021;116(1):40. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: In Vivo, Produced, Microscopy