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Image Search Results
Journal: Scientific Reports
Article Title: The Role of Scleraxis in Fate Determination of Mesenchymal Stem Cells for Tenocyte Differentiation
doi: 10.1038/srep13149
Figure Lengend Snippet: ( A ) Enhanced Smad 1/5/8 phosphorylation induced by combined treatment of BMP-12 and cyclic stretch loading. Cells under indicated treatment were harvested at 1 hour and 2 hours post-treatment to analyze the levels of Smad1/5/8 phosphorylation by Western blot. ( B–E ) Activation of Smad8 in C3H10T1/2 stem cells in response to BMP-12 and stretch loading. Cells were transfected with siRNA against Smad1 , Smad5 , Smad8 , or control siRNA, (siScr) followed by treatment of BMP-12 or loading or both, and protein phosphorylation analyzed by Western blot. C: Control, B: BMP-12 only, S: stretch loading only, B + S: BMP-12+stretch loading, Scr: Scramble of siRNA, siS1: siRNA-Smad1, siS5: siRNA-Smad5, siS8: siRNA-Smad8 .
Article Snippet: C3H10T1/2 cells were transfected with either Scx -siRNA, Tnmd -siRNA, Smad1 -siRNA, Smad5 -siRNA, or
Techniques: Phospho-proteomics, Western Blot, Activation Assay, Transfection, Control