Journal: Bioengineered

Article Title: Circadian Period 2 (Per2) downregulate inhibitor of differentiation 3 (Id3) expression via PTEN/AKT/Smad5 axis to inhibits glioma cell proliferation

doi: 10.1080/21655979.2022.2074107

Figure Lengend Snippet: Per2 inhibit Id3 expression via regulating PTEN/AKT/Smad5 signaling pathway. The protein levels of PTEN, AKT, p-AKT(T308), p-AKT(S473), Smad5, p-Smad5, GAPDH were analyzed by Western blot in U87 and U251 cells.

Article Snippet: SDS-PAGE was used to separate the protein extracts, which were then transferred onto polyvinylidene difluoride (PVDF) membranes, followed by incubation with the following primary antibodies overnight on 4°C shakers: Per2 (Abcam, ab179813), Id3 (Abbkine, ABP58864), PTEN (BIOSS, bsm-52369R), AKT (Proteintech, 60203-2-Ig), p-AKT (T308) (Proteintech, 29163-1-AP), p-AKT (S473) (Proteintech, 80455-1-RR), Smad5 (BIOSS, bs-13890R), p-Smad5 (BIOSS, bs-19918R), GAPDH (BIOSS, bsm-33033M) and β-tubulin (Zen Bioscience Chengdu, China, 200608).

Techniques: Expressing, Western Blot

Journal: Molecular Oncology

Article Title: Single‐cell protein profiling defines cell populations associated with triple‐negative breast cancer aggressiveness

doi: 10.1002/1878-0261.13365

Figure Lengend Snippet: Overview of antibodies and reagents used in study.

Article Snippet: pSmad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) , D5B10 , 151Eu , Cell Signaling , #12428; 191209.

Techniques: Cytometry, Marker