smad1 Search Results


smad proteins  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc smad proteins
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Smad Proteins, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/Smad+1%2F5%2F9+Antibody+Sampler+Kit/pmc06064884-92-7-19
Average 94 stars, based on 1 article reviews
smad proteins - by Bioz Stars, 2026-10
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phospho smad1 5 rabbit monoclonal antibody antipmad  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho smad1 5 rabbit monoclonal antibody antipmad
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Phospho Smad1 5 Rabbit Monoclonal Antibody Antipmad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
phospho smad1 5 rabbit monoclonal antibody antipmad - by Bioz Stars, 2026-10
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rabbit anti phospho smad1 5 9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti phospho smad1 5 9
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Rabbit Anti Phospho Smad1 5 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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anti phospho smad 1 5  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti phospho smad 1 5
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Anti Phospho Smad 1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/Phospho-Smad1+(Ser463%2F465)%2F+Smad5+(Ser463%2F465)%2F+Smad9+(Ser465%2F467)+Rabbit+mAb/pmc11929866-236-6-9
Average 95 stars, based on 1 article reviews
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p smad1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc p smad1
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
P Smad1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/Phospho-Smad1+(Ser206)+Antibody/pmc06989010-54-21-37
Average 93 stars, based on 1 article reviews
p smad1 - by Bioz Stars, 2026-10
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smad1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc smad1
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Smad1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/Smad1+XP+Rabbit+mAb/pm34653364-776-17-19
Average 95 stars, based on 1 article reviews
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anti phosphorylated smad 1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti phosphorylated smad 1
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Anti Phosphorylated Smad 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad1 5 ser463 465 41d10 rabbit mab  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phospho smad1 5 ser463 465 41d10 rabbit mab
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Phospho Smad1 5 Ser463 465 41d10 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/Phospho-Smad1%2F5+(Ser463%2F465)+Rabbit+mAb/bio_rxiv__2021__10__01__462731-70-8-13
Average 95 stars, based on 1 article reviews
phospho smad1 5 ser463 465 41d10 rabbit mab - by Bioz Stars, 2026-10
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anti p smad1  (Proteintech)
94
Proteintech anti p smad1
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Anti P Smad1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smad1/SMAD1+Antibody/ppr0953879-92-62-64
Average 94 stars, based on 1 article reviews
anti p smad1 - by Bioz Stars, 2026-10
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anti smad1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti smad1
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Anti Smad1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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short hairpin shrna lentiviral particles  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology short hairpin shrna lentiviral particles
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Short Hairpin Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti smad1 2 3 mab h 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti smad1 2 3 mab h 2
Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target <t>proteins</t> <t>Rictor</t> and <t>SMAD</t> ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.
Anti Smad1 2 3 Mab H 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target proteins Rictor and SMAD ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Inhibition of MicroRNA‐155 Supports Endothelial Tight Junction Integrity Following Oxygen‐Glucose Deprivation

doi: 10.1161/JAHA.118.009244

Figure Lengend Snippet: Efficiency of microRNA‐155 (miR‐155) inhibition and overexpression. A, Diagram describing the experimental setup. Human primary brain microvascular endothelial cells (HBMECs) seeded in cell culture inserts were subjected to 3 hours of oxygen‐glucose deprivation (OGD) and returned back to the normal cell culture conditions; 24 hours later, the cells were transfected with miR‐155 inhibitor, mimic, or appropriate scrambled oligonucleotide. Cells were analyzed at 48 hours after the transfection. B, Fluorescence confocal microscopy of HBMEC s transfected with fluorescein‐labeled miR‐155 inhibitor control (green dots) and stained for actin with rhodamine‐conjugated fluorescent phalloidin. Left panel: orthogonal image projection verifies that fluorescent probes were incorporated within the cell. Bar: 10 μm. C and D, miR‐155 PCR analysis. Total RNA was isolated from the cells subjected to OGD and transfected with the following oligonucleotides: miR‐155 mimic ( OGD /M; grey bar); mimic control ( OGD / MC; grey bar with white stripes); specific miR‐155 inhibitor ( OGD /I; black bar); and control inhibitor ( OGD / IC ; black bar with white stripes). C, P =0.002, (D) P =0.029, Mann–Whitney (Wilcoxon) test. n=3 (for OGD / MC and OGD / IC groups) and n=4 (for OGD /I and OGD /M groups) independent experiments. E, Validation of miR‐155 inhibition by the quantitative assessment of miR‐155 direct target proteins Rictor and SMAD ‐1. Optical density of the protein bands was measured using ImageJ software, normalized to GAPDH density in every sample, and expressed as average relative density values. Protein levels were compared between OGD /I (black bars) and OGD / IC (black bars with white stripes) cell lysates. P =0.029, Mann–Whitney (Wilcoxon) test, n=3 independent experiments per group. Representative immunoblots demonstrate Rictor and SMAD ‐1 protein expression (3 bands per group); GAPDH was used as a loading control. Error bars: SEM ; * P <0.05; ** P <0.01.

Article Snippet: SMAD‐1 and Rictor proteins were detected using SMAD proteins (Cat# 12656) and mTOR pathway (Cat# 9964) antibody sampler kits (Cell Signaling).

Techniques: Inhibition, Over Expression, Cell Culture, Transfection, Fluorescence, Confocal Microscopy, Labeling, Control, Staining, Isolation, MANN-WHITNEY, Biomarker Discovery, Software, Western Blot, Expressing