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Becton Dickinson
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Image Search Results
Journal: Disease Markers
Article Title: Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression
doi: 10.1155/2022/8446629
Figure Lengend Snippet: Exosomes rich in CBFB promoted the generation of CAF-like phenotype. (a) HMF3A cells treated with CBFB-rich exosome derived from bone metastatic patients with breast cancer (exosomes) exhibited significant increases in CAF markers, including vimentin (Vim), FAP, and α -SAM, and decreases in cytokeratin (KRT1) in immortalized human mammary fibroblast cell line HMF3A. (b) The CAF-like (VIM+/ α SMA+) cell percentages increased following the exosomes treatment. (c) The Western blot results demonstrated CBFB upregulation in the HMF3A cells treated with exosomes. HMF3A-exosomes cocultured with low-metastatic-potential breast cancer cell lines exhibited enhanced metastatic potential. Both low-metastatic-potential breast cancer cell lines, MCF12A and T47D, exhibited enhanced migration (d) and invasion (e) abilities compared with their control counterparts. Representative images of invasion are shown. Scale bars: 200 μ m. (f) ELISA analyses of exosome-induced CAFs. CAFs generated from exosome coculture demonstrated an increased secretion of IL-6 and OPN into the culture medium. ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Article Snippet:
Techniques: Derivative Assay, Western Blot, Migration, Enzyme-linked Immunosorbent Assay, Generated