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Image Search Results
Journal: iScience
Article Title: The role of long noncoding RNA Nron in atherosclerosis development and plaque stability
doi: 10.1016/j.isci.2022.103978
Figure Lengend Snippet: Nron overexpression induces a highly characteristic architecture of more-vulnerable plaques 6-Week male ApoE −/- mice were fed a western diet for 16 weeks. Mice were intravenously administered with adenovirus (Ad-SM22-EV or Ad-SM22-Nron) every two weeks in the last 8 weeks before being sacrificed. (A) Oil Red O staining of thoracoabdominal aorta, and lipid accumulation was quantified as percentage of total surface area of aorta. (B) Images and quantification of Oil Red O staining in lesions of aortic sinus. (C) Representative images of aortic sinus for H&E (HE), Masson’s trichrome (Masson), Elastica van Gieson (EVG), smooth muscle cells (α-SMA), and macrophages (F4/80). Arrows in EVG staining panels indicate ruptures of elastic fibers of tunica media. (D) Quantification of collagen content, fibrous cap area, necrotic core area, ruptures of elastic fibers, macrophage content, and smooth muscle cell content. Data are expressed as mean ± SEM (n = 12 per group). Statistical analysis was performed using Student’s t test. ∗p <0.05 vs. Ad-SM22-EV group.
Article Snippet: Primary antibodies specific for
Techniques: Over Expression, Western Blot, Staining
Journal: iScience
Article Title: The role of long noncoding RNA Nron in atherosclerosis development and plaque stability
doi: 10.1016/j.isci.2022.103978
Figure Lengend Snippet: Knockdown of Nron suppresses the development of atherosclerosis and increases the stability of atherosclerotic plaques 6-Week male ApoE −/- mice were fed a western diet for 16 weeks. Mice received a single intravenous injection of adeno-associated virus 2/8 (rAAV-shNC or rAAV-shNron) after feeding for 4 weeks. (A) Oil Red O staining of thoracoabdominal aorta, and lipid accumulation was quantified as percentage of total surface area of aorta. (B) Images and quantification of Oil Red O staining in lesions of aortic sinus. (C) Representative images of aortic sinus for H&E (HE), Masson’s trichrome (Masson), Elastica van Gieson (EVG), smooth muscle cells (α-SMA), and macrophages (F4/80). (D) Quantification of collagen content, fibrous cap area, necrotic core area, ruptures of elastic fibers, macrophage content, and smooth muscle cell content. Data are expressed as mean ± SEM (n = 12 per group). Statistical analysis was performed using Student’s t test. ∗p <0.05 vs. rAAV-shNC group.
Article Snippet: Primary antibodies specific for
Techniques: Western Blot, Injection, Staining
Journal: iScience
Article Title: The role of long noncoding RNA Nron in atherosclerosis development and plaque stability
doi: 10.1016/j.isci.2022.103978
Figure Lengend Snippet:
Article Snippet: Primary antibodies specific for
Techniques: Recombinant, Plasmid Preparation, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Silver Staining, In Vitro, TUNEL Assay, Software