Journal: Biotechnology progress

Article Title: Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

doi: 10.1002/btpr.3452

Figure Lengend Snippet: FIGURE 1 Schematic diagram of 2D gel quadrants. This figure serves as a visual guide to the four quadrant locations assigned to spots in 2D gels during SpotMap analysis and their associated relative MW and pI. Quadrants are automatically assigned by splitting the total number of spots approximately in half vertically and horizontally on the gel.

Article Snippet: Gel images were uploaded to the SpotMap (TotalLab, Newcastle upon Tyne, UK) software for analysis.

Techniques: Two-Dimensional Gel Electrophoresis

Journal: Biotechnology progress

Article Title: Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

doi: 10.1002/btpr.3452

Figure Lengend Snippet: FIGURE 2 2D gel images of CHO HCCF with product imaged using silver stain (a) [531 spots] and SYPRO Ruby (b) [948 spots]. Spots were assigned using the SpotMap software and are shown in cyan.

Article Snippet: Gel images were uploaded to the SpotMap (TotalLab, Newcastle upon Tyne, UK) software for analysis.

Techniques: Two-Dimensional Gel Electrophoresis, Silver Staining, Software

Journal: Biotechnology progress

Article Title: Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

doi: 10.1002/btpr.3452

Figure Lengend Snippet: FIGURE 3 Representative 2D gel images of null CHO HCCF imaged using silver stain (a) [602 spots] and SYPRO Ruby (b) [957 spots]. Spots were assigned using the SpotMap software and are shown in cyan.

Article Snippet: Gel images were uploaded to the SpotMap (TotalLab, Newcastle upon Tyne, UK) software for analysis.

Techniques: Two-Dimensional Gel Electrophoresis, Silver Staining, Software

Journal: bioRxiv

Article Title: An orthogonal TRAP enables intersectional genetic access to activated neurons in the mouse brain

doi: 10.64898/2025.11.30.691330

Figure Lengend Snippet: a. Schematic describing design of the X-TRAP knockin mouse, in which a cassette containing the "X-On Switch" and FlpO is knocked in immediately upstream of the start codon of the Fos gene. Activity-dependent transcription at the Fos locus produces FlpO mRNA that is not translated into protein due to lack of an in-frame start codon. Treatment with branaplam causes alternative splicing to produce a transcript that contains a start codon and is translated. b. The X-TRAP system, when used in conjunction with the TRAP2 allele, enables intersectional labeling of activated neurons in response to sets of stimuli. Top: Neurons expressing FOS in response to three different stimuli are visualized by labeling neurons with X-TRAP, TRAP2, and immunostaining for FOS. Bottom: When used in conjunction with reporter mice or viruses, this enables genetic access to cells that are the intersection of neurons activated by different stimuli. c. Concentration of branaplam in the blood after IP injection (25 mg/kg). d. Concentration of branaplam in the blood and brain after IP injection (25 mg/kg). e. Pharmacokinetic parameters for branaplam in blood and brain. f-I. IP injection of branaplam (25 mg/kg) has no effect on food intake ( f ), latency to eat ( g ), water consumption ( h ), or latency to drink (i). Data are mean ± sem.

Article Snippet: For the X-TRAP experiments, Branaplam hydrochloride (10 mg, MedChemExpress) was dissolved in 1 mL of an 18% solution of 2-hydroxypropyl-beta-cyclodextrin (HP-b-CD, Sigma Aldrich).

Techniques: Knock-In, Activity Assay, Alternative Splicing, Labeling, Expressing, Immunostaining, Concentration Assay, Injection

Journal: bioRxiv

Article Title: An orthogonal TRAP enables intersectional genetic access to activated neurons in the mouse brain

doi: 10.64898/2025.11.30.691330

Figure Lengend Snippet: a. Schematic for measuring homecage recombination with X-TRAP. b. There is no detectable Flp recombination in X-TRAP mice without branaplam treatment. Two coronal sections are shown with insets of pons (PonG) and external cuneate (ECu) nuclei. c. Homecage recombination after branaplam treatment in coronal sections matched to panel b. d. Top 20 regions with most homecage recombination in branaplam treated mice. e. Schematic for comparing baseline recombination between XTRAP and TRAP. f. X-TRAP and TRAP recombination, and endogenous FOS expression, in six brain regions. g. % X-TRAP+ cells that are also TRAP+. h. % TRAP+ cells that are also X-TRAP+ i. % dual labelled (X-TRAP+ and TRAP+) cells that are also FOS+. j. % FOS + that are also labelled by both X-TRAP and TRAP. k. Three brain regions that show higher baseline recombination with TRAP than X-TRAP. I. The number of cells labelled in the brain regions from k. Scale bars = 1 mm (panels b and c) or 0.1 mm (panels f and k). NS, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are mean ± sem.

Article Snippet: For the X-TRAP experiments, Branaplam hydrochloride (10 mg, MedChemExpress) was dissolved in 1 mL of an 18% solution of 2-hydroxypropyl-beta-cyclodextrin (HP-b-CD, Sigma Aldrich).

Techniques: Expressing

Journal: bioRxiv

Article Title: An orthogonal TRAP enables intersectional genetic access to activated neurons in the mouse brain

doi: 10.64898/2025.11.30.691330

Figure Lengend Snippet: a. Homecage recombination without (left) and with (right) branaplam treatment. Shown is Tomato fluorescence in an X-TRAP mouse crossed to a Flp-Tomato reporter. Note there are no cells without branaplam, and only sparse recombination after branaplam. The numbers at left are the distance from bregma of the slice. Scale bars are 1 mm. b. Homecage recombination with X-TRAP (left) versus TRAP (right) in the same mouse. Note that there is generally less recombination with X-TRAP. Scale bars are 0.1 mm.

Article Snippet: For the X-TRAP experiments, Branaplam hydrochloride (10 mg, MedChemExpress) was dissolved in 1 mL of an 18% solution of 2-hydroxypropyl-beta-cyclodextrin (HP-b-CD, Sigma Aldrich).

Techniques: Fluorescence

Journal: bioRxiv

Article Title: An orthogonal TRAP enables intersectional genetic access to activated neurons in the mouse brain

doi: 10.64898/2025.11.30.691330

Figure Lengend Snippet: a. Fos XTRAP was crossed to the ai224 reporter line, in which Flp recombination induces nuclear expression of Tomato. Mice were injected with branaplam (25 mg/kg, IP) and an hour later challenged with one of the following stimuli: Hypertonic saline (2M NaCl, 0.25 ml), ghrelin (∼2 mg/kg), haloperidol (−2 mg/kg), or control saline (0.15M NaCl, 0.1 ml). Mice were sacrificed two weeks later and Tomato expression was quantified throughout the brain. b. Tomato expression of the SFO, ARC, and CPu in response to the indicated stimuli. (Left) Example image from one mouse in red. (Right) Overlay of Tomato positive cells from n=4 mice. c. Tomato expression in 63 midbrain and forebrain structures response to the stimuli indicated. Data are presented as the ratio of labeled cells compared to control (stimulus/vehicle). Key brain regions known to respond the indicated stimulus are highlighted in red. Scale bars are 0.1 mm. Data are mean ± sem.

Article Snippet: For the X-TRAP experiments, Branaplam hydrochloride (10 mg, MedChemExpress) was dissolved in 1 mL of an 18% solution of 2-hydroxypropyl-beta-cyclodextrin (HP-b-CD, Sigma Aldrich).

Techniques: Expressing, Injection, Saline, Control, Labeling