Journal: World Journal of Surgical Oncology

Article Title: SLUG is activated by nuclear factor kappa B and confers human alveolar epithelial A549 cells resistance to tumor necrosis factor-alpha-induced apoptosis

doi: 10.1186/1477-7819-11-12

Figure Lengend Snippet: Effect of TNF-α on Bcl-2 expression. Lung cancer cells were treated with 20 ng/ml TNF-α. ( A ) Time course of Bcl-2 mRNA induction by TNF-α in A549 lung cancer cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was loaded as control. ( B ) Western blot analysis of Bcl-2 protein expression in A549 cells followed TNF-α treatment for different times. ( C ) Wedelolactone-pretreated A549 lung cancer cells were transfected with SLUG or the control scrambled siRNA, and then treated with TNF-α for 48 h. SLUG, Bcl-2 and p-P65 expression was analyzed by western blot. ( D ) Wedelolactone-pretreated A549 lung cancer cells were transfected with SLUG or the control scrambled siRNA, and then treated with TNF-α for 24 h. SLUG, Bcl-2 and p-P65 expression was analyzed by reverse transcription PCR.

Article Snippet: Transfection was performed with Lipofectamine 2000 (Invitrogen, California, USA) following the manufacturer’s instructions. siRNA transfection was performed 24 h before 20 ng of TNF-α treatment. siRNA duplexes, including PUMA siRNA, SLUG siRNA and Bcl-2 siRNA and the control scrambled siRNA were from Santa Cruz Biotechnology.

Techniques: Expressing, Control, Western Blot, Transfection, Reverse Transcription

Journal: World Journal of Surgical Oncology

Article Title: SLUG is activated by nuclear factor kappa B and confers human alveolar epithelial A549 cells resistance to tumor necrosis factor-alpha-induced apoptosis

doi: 10.1186/1477-7819-11-12

Figure Lengend Snippet: Effect of p53 upregulated modulator of apoptosis (PUMA), Bcl-2 and SLUG on apoptosis of TNF-α-induced apoptosis. Apoptpsis of A549 cells following TNF-α treatment were analyzed after treated with wedelolactone, PUMA, bcl-2 or SLUG siRNA.

Article Snippet: Transfection was performed with Lipofectamine 2000 (Invitrogen, California, USA) following the manufacturer’s instructions. siRNA transfection was performed 24 h before 20 ng of TNF-α treatment. siRNA duplexes, including PUMA siRNA, SLUG siRNA and Bcl-2 siRNA and the control scrambled siRNA were from Santa Cruz Biotechnology.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: SLUG is activated by nuclear factor kappa B and confers human alveolar epithelial A549 cells resistance to tumor necrosis factor-alpha-induced apoptosis

doi: 10.1186/1477-7819-11-12

Figure Lengend Snippet: PUMA expression was analyzed by SLUG siRNA treatment following TNF-α treatment.

Article Snippet: Transfection was performed with Lipofectamine 2000 (Invitrogen, California, USA) following the manufacturer’s instructions. siRNA transfection was performed 24 h before 20 ng of TNF-α treatment. siRNA duplexes, including PUMA siRNA, SLUG siRNA and Bcl-2 siRNA and the control scrambled siRNA were from Santa Cruz Biotechnology.

Techniques: Expressing