Journal: Cell Reports Medicine

Article Title: Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors

doi: 10.1016/j.xcrm.2021.100457

Figure Lengend Snippet:

Article Snippet: Anti-human CD98 - PE-Vio770 , Miltenyi Biotec , Cat# 130-105-710, RRID: AB_2659686.

Techniques: Control, Recombinant, Staining, Transfection, Enzyme-linked Immunosorbent Assay, Luciferase, Gene Expression, Cloning, Software

Journal: Behavioural brain research

Article Title: Prenatal stress-induced disruptions in microbial and host tryptophan metabolism and transport

doi: 10.1016/j.bbr.2021.113471

Figure Lengend Snippet: Taqman Mouse-Specific Gene Expression Assays.

Article Snippet: SLC7A5 , Mm00441516_m1.

Techniques: Gene Expression

Journal: Behavioural brain research

Article Title: Prenatal stress-induced disruptions in microbial and host tryptophan metabolism and transport

doi: 10.1016/j.bbr.2021.113471

Figure Lengend Snippet: SLC6A4 (SERT) is a transporter of 5-HT. Increased 5-HT induces AhR. SLC3A2 and SLC7A5 heterodimerize to form LAT-1 and act as a Trp transporter. Trp can be metabolized to indole, which can act as a ligand for AhR. AhR, when bound to a ligand such as indole, can induce expression of Cyp1 genes (Cyp1a1, Cyp1b1). This figure was created in BioRender.

Article Snippet: SLC7A5 , Mm00441516_m1.

Techniques: Expressing

Journal: Behavioural brain research

Article Title: Prenatal stress-induced disruptions in microbial and host tryptophan metabolism and transport

doi: 10.1016/j.bbr.2021.113471

Figure Lengend Snippet: (A) Distal colon Tdo2 (p < 0.05) was increased over the course of the assessment. (B) Cyp1a1 at PNW1 (p < 0.05), (C) 5-ht2br at PNW1 (p < 0.05), (D) Slc7a5 at PNW3 (p = 0.0556), and (E) Slc3a2 at PNW5 (p = 0.0655), were altered in PNS offspring compared to control. Data are mean ± SEM. All data are presented as 2−ΔΔCT (fold-change compared to control). For each time point, one male and one female were used from each litter, and then averaged after statistical testing for sex revealed no effects; n = 5–7. * = p < 0.05.

Article Snippet: SLC7A5 , Mm00441516_m1.

Techniques: Control

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: SLC7A5 proteins are stained in brown; cell nuclei are counter stained purple. Original magnifications: 400×. ( A ) In benign mucosa, SLC7A5 expression is negative to very weak and localized to the cytoplasmic membrane or stromal cytoplasm. ( B-F ) In colorectal cancer, SLC7A5 is detected in tumor membrane at various levels, ranging from weakly positive tumor cells to strong expression in most cells. ( G, H ) SCL7A5 expression range distribution in 522 earl-stage cancers ( G ) and 81 late-stage cancers ( H ). Total weighted H-score of 40 is used as a cut-off (vertical red line). SLC7A5 expression in B, C are considered low and in D-F are considered high.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Staining, Expressing, Membrane

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Correlation of SLC7A5 protein expression with clinicopathological features of colorectal cancer patients.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: High expression refers to cancer tissue with immunohistochemical staining H-score ≥40. ( A, B ) In early-stage cancers, SLC7A5 high expression is significantly correlated with longer survivals and better clinical outcome. Similar significant correlations hold in the MSS (microsatellite stability) and MSI (microsatellite instability) subtypes. ( C, D ) In late-stage cancers, SLC7A5 expression is not correlated with overall or disease-free survivals.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Univariate and multivariate analyses of patient survival in early-stage colorectal cancer.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: The analysis was based on the mass spectrometry protein sequencing data from the CPTAC database. The Y-axis represents SLC7A5 protein level z-scores. SLC7A5 protein expression levels are significantly higher in BRAF-mutated tumors than wild-type tumors.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Mass Spectrometry, Sequencing, Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: ( A ) The analysis of the SLC7A5 protein and mRNA expressions in the 86-case CPTAC colorectal cohort revealed significant consistency between protein and gene transcription levels. Spearman’s rank correlation coefficient r = 0.5360, p<0.0001. ( B ) SLC7A5 mRNA expression showed the most significant positive correlation with SLC3A2 mRNA expression among all gene transcripts in the 592-case TCGA colorectal cancer cohort. Spearman’s rank correlation coefficient r = 0.7291, p<0.0001.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Only those with Spearman’s rank correlation coefficient >0.4 were included in the network. Only interactions with high confidence (0.7/1.0) are shown in the figure. Amino acid transporters are colored (red). According to Gene Ontology Biological Process annotation, the SLC7A5-network is significantly enriched in ncRNA metabolic process (aqua), RNA metabolic process (blue), and cell cycle (green). Proteins/genes colored yellow are also commonly found in lymphocytes.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques:

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: (A) Ultra-structure of hepatocytes near cysts at different stages of infection with PSCs (0, 1, 3, and 6 months); mitochondria are indicated by white arrows,PSC are indicated by black arrows( n = 3 rats/group, scale bars represent 2 μm and 1 μm). ( B ) Fe 2+ , ( C ) GSH, ( D ( a , b )) ROS, ( E ) MDA, ( F ) SOD, and ( G ) LDH of hepatocytes near cysts at different stages of infection with PSCs. ( H ( a , b )) Western blotting of protein expression levels of TFRC, GPX4, FTH1, NOX1, SLC3A2, and SLC7A11 in ferroptosis signaling pathway in rat liver tissue at different stages of infection with PSCs ( n = 3 rats/group)); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Infection, Western Blot, Expressing

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: ( A ) Fe 2+ in normal BRL cells, PSCs and BRL co-cultured cells, and PSCs +Ferrostatin-1 and BRL co-cultured cells. ( B ) GSH, ( C ) GSSH, ( D ) GSH/GSSH, ( E ) SOD, ( F ) LDH, and ( G ) MDA concentration detection ( n = 6 rats/group). ( H ( a , b )) Western-blotting detection of expression levels of cell ferroptosis signaling pathway proteins in each group, such as TFRC, GPX4, FTH1, NOX1, SLC3A2, andSLC7A11 ( n = 3 rats/group); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Cell Culture, Concentration Assay, Western Blot, Expressing

Journal: bioRxiv

Article Title: MYC disrupts transcriptional and metabolic circadian oscillations in cancer and promotes enhanced biosynthesis

doi: 10.1101/2023.01.03.522637

Figure Lengend Snippet: A-C. U2OS MYC-ER ( A ), SHEP N-MYC-ER, ( B ), and SKNAS N-MYC-ER ( C ) were treated with ethanol control (MYC-OFF) or 4-hydroxytamoxifen (MYC-ON) (4OHT) to activate MYC, and entrained with dexamethasone, and after 24 hours, protein was collected every 4 hours for the indicated time period. Protein lysates were prepared to preserve protein glycosylation (see Methods), and immunoblot was performed for the indicated proteins. For some targets [4F2hc, LAT1, REV-ERBα (abbreviated REVα)], a darker exposure (‘dark’) and lighter exposure (‘light’) of the same blot are presented. For GLUT1, # indicates a non-specific band. Some samples (CT26 for U2OS, CT32 for SHEP and SKNAS) were treated with PNGase-F prior to immunoblot to remove glycosylation marks. Note that the PNGase-F lanes have less protein loaded than the other lanes. Data represent n=2 biological replicates for U2OS and one each for SHEP and SKNAS. D. On-cell western of U2OS MYC-ER cells ± MYC for LAT1. U2OS MYC-ER cells were grown on a 24-well tissue culture plate ± 4OHT for 48 hours, fixed with formaldehyde but not permeabilized, and then stained with the indicated antibody or IgG control. CellStain 700 indicates cell density in each well. Data represent at least two independent experiments of 6 biological replicate wells each. E. Quantitation of LAT1 or IgG from ( D ). * indicates P < 0.00001 by Welch’s Corrected Student’s T-test. F. LC-Mass spectrometry was performed on U2OS MYC-ER treated ± 4OHT for at least 48 hours. N=25 circadian timepoints for MYC-OFF and MYC-ON were averaged as biological replicates, normalized to cell number for each collection. * indicates p < 0.05 by Welch’s Corrected Student’s T-test.

Article Snippet: Wells were washed with tris-buffered saline, and stained with primary antibodies mouse anti-LAT1 BU53 (Novus NBP2-50465AF647) or mouse IgG2A isotype control (Novus IC003R), and secondary antibodies goat anti-mouse Alexa Flour 790 (Thermo Scientific A11357) or CellTag 700 Stain (Licor 926-41090), which is used to quantify total cell number and intensity.

Techniques: Control, Western Blot, Staining, Quantitation Assay, Mass Spectrometry