Journal: Cell Reports Medicine

Article Title: Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors

doi: 10.1016/j.xcrm.2021.100457

Figure Lengend Snippet:

Article Snippet: Anti-human CD98 - PE-Vio770 , Miltenyi Biotec , Cat# 130-105-710, RRID: AB_2659686.

Techniques: Control, Recombinant, Staining, Transfection, Enzyme-linked Immunosorbent Assay, Luciferase, Gene Expression, Cloning, Software

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: (A) Ultra-structure of hepatocytes near cysts at different stages of infection with PSCs (0, 1, 3, and 6 months); mitochondria are indicated by white arrows,PSC are indicated by black arrows( n = 3 rats/group, scale bars represent 2 μm and 1 μm). ( B ) Fe 2+ , ( C ) GSH, ( D ( a , b )) ROS, ( E ) MDA, ( F ) SOD, and ( G ) LDH of hepatocytes near cysts at different stages of infection with PSCs. ( H ( a , b )) Western blotting of protein expression levels of TFRC, GPX4, FTH1, NOX1, SLC3A2, and SLC7A11 in ferroptosis signaling pathway in rat liver tissue at different stages of infection with PSCs ( n = 3 rats/group)); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Infection, Western Blot, Expressing

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: ( A ) Fe 2+ in normal BRL cells, PSCs and BRL co-cultured cells, and PSCs +Ferrostatin-1 and BRL co-cultured cells. ( B ) GSH, ( C ) GSSH, ( D ) GSH/GSSH, ( E ) SOD, ( F ) LDH, and ( G ) MDA concentration detection ( n = 6 rats/group). ( H ( a , b )) Western-blotting detection of expression levels of cell ferroptosis signaling pathway proteins in each group, such as TFRC, GPX4, FTH1, NOX1, SLC3A2, andSLC7A11 ( n = 3 rats/group); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Cell Culture, Concentration Assay, Western Blot, Expressing

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer *

doi: 10.1074/mcp.M115.051524

Figure Lengend Snippet: Correlations of SLC3A2, STMN1, and TAGLN2 protein expression (IHC scores) with clinicopathological characteristics in tissue specimens

Article Snippet: The urinary concentrations of the four candidate proteins were measured using commercial ELISA kits for SLC3A2 (Cusabio, Wuhan, China), SFN (Cusabio, Wuhan, China) and STMN1 (USCN Life Sciences, Wuhan, China), and an ELISA developed in house for TAGLN2.

Techniques: Expressing

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer *

doi: 10.1074/mcp.M115.051524

Figure Lengend Snippet: Details of biomarker discovery data and verification of seven selected protein candidates in tissue and urine specimens

Article Snippet: The urinary concentrations of the four candidate proteins were measured using commercial ELISA kits for SLC3A2 (Cusabio, Wuhan, China), SFN (Cusabio, Wuhan, China) and STMN1 (USCN Life Sciences, Wuhan, China), and an ELISA developed in house for TAGLN2.

Techniques: Biomarker Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Marker

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer *

doi: 10.1074/mcp.M115.051524

Figure Lengend Snippet: Up-regulation of A, SLC3A2; B, STMN1; and C, TAGLN2 in individual bladder tumor tissue specimens determined by IHC.

Article Snippet: The urinary concentrations of the four candidate proteins were measured using commercial ELISA kits for SLC3A2 (Cusabio, Wuhan, China), SFN (Cusabio, Wuhan, China) and STMN1 (USCN Life Sciences, Wuhan, China), and an ELISA developed in house for TAGLN2.

Techniques:

Journal: Materials Today Bio

Article Title: A bioengineered tumor matrix-based scaffold for the evaluation of melatonin efficacy on head and neck squamous cancer stem cells

doi: 10.1016/j.mtbio.2024.101246

Figure Lengend Snippet: HNSCC microenvironment model. (A) Characterization of Cal-27 CSCs. Image of tumorspheres grown in suspension with serum-free spheres medium (10×). Cytometry histograms of CD98 and CD44 CSCs cell-membrane markers expression. Percentage of ALDH1 activity of cells grown in monolayer or in suspension (cytometry histograms besides). (B) Schematic representation of the TME components embedded in the hydrogel. Confocal representative images of cell viability of tumorspheres, FBs and MSCs (stroma) and the co-culture of stroma and tumorspheres (TME) cultured in the hydrogel. Living cells shown in green and nuclei of death cells in red. Scale bar: 200 μm. (C) Proliferation assay of the tumorspheres, FBs and MSCs (stroma) and the co-culture of stroma and tumorspheres (TME) cultured in the hydrogel. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cells were centrifuged followed by the addition of fluorochrome-conjugated monoclonal antibodies for CD98 and CD44 (Miltenyi Biotec) according to the manufacturer's instructions and incubated at 4 °C in the dark for 12 min. After adding BSA, cells were centrifugated and resuspended in PBS and analyzed by flow cytometry in a FACSCanto II cytometer (BD Biosciences).

Techniques: Suspension, Cytometry, Membrane, Expressing, Activity Assay, Co-Culture Assay, Cell Culture, Proliferation Assay

Journal: Frontiers in Microbiology

Article Title: The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells

doi: 10.3389/fmicb.2021.615165

Figure Lengend Snippet: CHIKV envelope association with the CD147 complex (A) Scheme of the used AP-MS approach. (B) CHIKV envelope Interaction partners of interest are listed. Examination of their plasma membrane presence (PM), presence of a transmembrane domain (TM), and MiST score (MiST) are indicated. Components of the CD147 protein complex retrieved in the CHIKV envelope affinity purification are indicated. (C–F) Western Blot of affinity purification of Strep-tagged Envelope protein. Detection of E2 and the interactors CD147, CD98, and SLC1A5. Affinity purification of untransfected cells (empty) were included as controls. On each blot input lysates of untransfected cells (empty) or E2 expressing cells (E2) and affinity purifications (AP) of the empty or E2 cells were loaded. For the CD147, CD98 and SLC1A5 blots 0.4 μl of input lysates and 10 μl of APs was loaded. For the E2 blot 5 μl of input lysates and 10 μl of APs was loaded.

Article Snippet: Membranes were washed with PBS-T and incubated with primary antibody anti-CD147 (ab232967, Abcam), anti-ASCT2 (SLC1A5) (V501, Cell Signaling), anti-CD98 (12206-T62, sino biologicals), anti-E1, anti-Strep (ab184224 or ab180957, Abcam) and anti-E2 (NR44002, Bei Resources) overnight at 4°C.

Techniques: Membrane, Affinity Purification, Western Blot, Expressing

Journal: Neurobiology of disease

Article Title: Identifying disulfidptosis-related biomarkers in epilepsy based on integrated bioinformatics and experimental analyses.

doi: 10.1016/j.nbd.2025.106789

Figure Lengend Snippet: Fig. 2. Identification of DE-DRMs in individuals with EP. (A) The heatmap of expression levels for nine DE-DRMs. (B) The expression levels of 11 DRMs were exhibited between Ctrl and EP groups in boxplots. (C) Correlation analysis of nine DE-DRMs. Red and green colors represent positive and negative correlations, respectively. (D) The PPI analysis of DE-DMRs showed that SLC3A2 interacted with SLC7A11, while NDUFS1 interacted with NDUFA11, NUBPL, and LRPPRC. Abbreviations: DE-DRMs, differentially expressed disulfidptosis-related molecules; EP, epilepsy; Ctrl, control; PPI, protein-protein interaction; SLC3A2, solute carrier family 3 member 2; SLC7A11, solute carrier family 7 member 11; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; NDUFA11, NADH:ubiquinone oxidoreductase subunit A11; NUBPL, NUBP iron‑sulfur cluster assembly factor, mitochondrial; LRPPRC, leucine rich pentatricopeptide repeat containing. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The primary antibodies for western blot were as follows: anti-SLC3A2 antibody (Santa, sc-390,154, dilution: 1:500), anti-SLC7A11 antibody (Abcam, ab307601, dilution: 1:1000), anti-NDUFS1 antibody (Abcam, ab185733, dilution: 1:1000), anti-LRPPRC antibody (Abcam, ab259927, dilution: 1:1000), antiNDUFA11 antibody (Abclonal, A16239, dilution: 1:1000), and antiNUBPL antibody (Boster, A10634–1, dilution: 1:1000).

Techniques: Expressing, Control

Journal: Neurobiology of disease

Article Title: Identifying disulfidptosis-related biomarkers in epilepsy based on integrated bioinformatics and experimental analyses.

doi: 10.1016/j.nbd.2025.106789

Figure Lengend Snippet: Fig. 8. The expression of DE-DRMs in seizures models (in vitro and in vivo). (A) Constructing the in vitro seizures model. The amplitude and frequency of neuronal APs in the Mg2+-free group were significantly increased (n = 6 in each group; Independent sample t-test; #, P < 0.01). (B) Protein expression of nine DE-DRMs in the in vitro seizures model. The expression of GYS1, NDUFS1, OXSM, LRPPRC, NDUFA11, NUBPL, NCKAP1, and SLC3A2 were not significantly changed in the Mg2+-free group, while the expression of SLC7A11 was significantly increased in Mg2+-free group (n = 6 in each group; Independent sample t-test; #, P < 0.01). (C) Con structing the in vivo seizures model. No epileptoid discharges were observed in six rats of the Ctrl group, while significant epileptoid discharges were observed in six rats of the PTZ group (scale: Y-axis,50uV; X-axis, 0.5 s). (D) Protein expression of nine DE-DRMs in vivo models. The expressions of GYS1, NDUFS1, OXSM, LRPPRC, NDUFA11, NUBPL, NCKAP1, and SLC3A2 were not significantly changed in PTZ group, while the expression of SLC7A11 was significantly increased in PTZ group (n = 6 in each group; Independent sample t-test; #, P < 0.01). Abbreviations: DE-DRMs, differentially expressed disulfidptosis-related molecules; APs, action potentials; GYS1, glycogen synthase 1; SLC3A2, solute carrier family 3 member 2; SLC7A11, solute carrier family 7 member 11; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; OXSM, 3-oxoacyl-ACP synthase, mitochondrial; LRPPRC, leucine rich pentatricopeptide repeat containing; NDUFA11, NADH:ubiquinone oxidoreductase subunit A11; NUBPL, NUBP iron‑sulfur cluster assembly factor, mitochondrial; NCKAP1, NCK associated protein 1; PTZ, pentylenetetrazol.

Article Snippet: The primary antibodies for western blot were as follows: anti-SLC3A2 antibody (Santa, sc-390,154, dilution: 1:500), anti-SLC7A11 antibody (Abcam, ab307601, dilution: 1:1000), anti-NDUFS1 antibody (Abcam, ab185733, dilution: 1:1000), anti-LRPPRC antibody (Abcam, ab259927, dilution: 1:1000), antiNDUFA11 antibody (Abclonal, A16239, dilution: 1:1000), and antiNUBPL antibody (Boster, A10634–1, dilution: 1:1000).

Techniques: Expressing, In Vitro, In Vivo

Journal: Neurobiology of disease

Article Title: Identifying disulfidptosis-related biomarkers in epilepsy based on integrated bioinformatics and experimental analyses.

doi: 10.1016/j.nbd.2025.106789

Figure Lengend Snippet: Fig. 9. Verifying the PPI in seizures models (in vivo and in vitro). (A-B) colocation analysis indicated that SLC7A11 colocalized with SLC3A2, NDUFS1 colocalized with LRPPRC, NDUFS1 colocalized with NUBPL, and NDUFS1 colocalized with NDUFA11 in both primary neurons and hippocampal tissue. (C–D) Pooled quan tification of protein immunoprecipitation showed a significant increment in the pull-down of SLC7A11 and a significant reduction in the pull-down of NDUFA11 in both the Mg2+-free group and PTZ group (n = 6 in each group; Independent sample t-test; #, P < 0.01). Abbreviations: PPI, protein-protein interaction; SLC3A2,solute carrier family 3 member 2; SLC7A11, solute carrier family 7 member 11; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; LRPPRC, leucine rich pentatricopeptide repeat containing; NUBPL, NUBP iron‑sulfur cluster assembly factor, mitochondrial; NDUFA11, NADH:ubiquinone oxidoreductase subunit A11; PTZ, pentylenetetrazol.

Article Snippet: The primary antibodies for western blot were as follows: anti-SLC3A2 antibody (Santa, sc-390,154, dilution: 1:500), anti-SLC7A11 antibody (Abcam, ab307601, dilution: 1:1000), anti-NDUFS1 antibody (Abcam, ab185733, dilution: 1:1000), anti-LRPPRC antibody (Abcam, ab259927, dilution: 1:1000), antiNDUFA11 antibody (Abclonal, A16239, dilution: 1:1000), and antiNUBPL antibody (Boster, A10634–1, dilution: 1:1000).

Techniques: In Vivo, In Vitro, Immunoprecipitation