sirna wdr5 Search Results


wdr5  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology wdr5
Intracellular adenosine decreases the methylation level of H3K4. a Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs pretreated with 2 mM MTA for 30 min ( n = 4). b Western blot detection of methylation levels of H3K4, H3K9, and H3K27 in TNF-α (10 ng/ml for 12 h)-treated HUVECs pretreated with 100 µM adenosine for 30 min ( n = 5). c ChIP assay showing H3K4 methylation on promoters of adhesion molecules in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs ( n = 3). d Western blot detection of H3K4me2 in HUVEC whole-protein lysate supplemented with 1 mg/ml SAM or SAM together with 10 µM adenosine for 60 min ( n = 4). e Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or <t>WDR5</t> siRNA ( n = 3). f Immunofluorescent ( IF ) staining of H3K4me2 on aortic endothelium (areas indicated with CD31 staining, red ) from TNF-α-treated ADK WT and ADK VEC-KO mice. L indicates luminal area of aorta ( scale bar , 100 µm). g Quantification of H3K4me2 on aortic endothelium from TNF-α-treated ADK WT and ADK VEC-KO mice ( n = 5 mice per group). h Western blot detection and densitometric quantification of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated MAECs isolated from ADK WT and ADK VEC-KO mice ( n = 4). i Western blot detection of H3K4 methylation in HUVECs. HUVECs, pretreated for 30 min with 10 µM NBMPR, were incubated with 100 µM adenosine for 30 min and then stimulated with TNF-α at 10 ng/ml for 4 h ( n = 3). j Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated A 2A R KD or Ctrl HUVECs pretreated with 100 µM adenosine for 30 min ( n = 4). k Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or A 2A R siRNA ( n = 4). For all bar graphs , data are the mean ± SEM, * P < 0.05 and ** P < 0.01 (unpaired, two-tailed Student’s t -test)
Wdr5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
wdr5 - by Bioz Stars, 2026-05
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sirnas for wdr5 (si-wdr5)  (VectorBuilder GmbH)
90
VectorBuilder GmbH sirnas for wdr5 (si-wdr5)
Intracellular adenosine decreases the methylation level of H3K4. a Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs pretreated with 2 mM MTA for 30 min ( n = 4). b Western blot detection of methylation levels of H3K4, H3K9, and H3K27 in TNF-α (10 ng/ml for 12 h)-treated HUVECs pretreated with 100 µM adenosine for 30 min ( n = 5). c ChIP assay showing H3K4 methylation on promoters of adhesion molecules in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs ( n = 3). d Western blot detection of H3K4me2 in HUVEC whole-protein lysate supplemented with 1 mg/ml SAM or SAM together with 10 µM adenosine for 60 min ( n = 4). e Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or <t>WDR5</t> siRNA ( n = 3). f Immunofluorescent ( IF ) staining of H3K4me2 on aortic endothelium (areas indicated with CD31 staining, red ) from TNF-α-treated ADK WT and ADK VEC-KO mice. L indicates luminal area of aorta ( scale bar , 100 µm). g Quantification of H3K4me2 on aortic endothelium from TNF-α-treated ADK WT and ADK VEC-KO mice ( n = 5 mice per group). h Western blot detection and densitometric quantification of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated MAECs isolated from ADK WT and ADK VEC-KO mice ( n = 4). i Western blot detection of H3K4 methylation in HUVECs. HUVECs, pretreated for 30 min with 10 µM NBMPR, were incubated with 100 µM adenosine for 30 min and then stimulated with TNF-α at 10 ng/ml for 4 h ( n = 3). j Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated A 2A R KD or Ctrl HUVECs pretreated with 100 µM adenosine for 30 min ( n = 4). k Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or A 2A R siRNA ( n = 4). For all bar graphs , data are the mean ± SEM, * P < 0.05 and ** P < 0.01 (unpaired, two-tailed Student’s t -test)
Sirnas For Wdr5 (Si Wdr5), supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirnas for wdr5 (si-wdr5)/product/VectorBuilder GmbH
Average 90 stars, based on 1 article reviews
sirnas for wdr5 (si-wdr5) - by Bioz Stars, 2026-05
90/100 stars
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wdr5 rat sirna oligo duplex  (OriGene)
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Wdr5 Rat 3 unique 27mer siRNA duplexes 2 nmol each
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wdr5 human sirna oligo duplex  (OriGene)
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WDR5 Human 3 unique 27mer siRNA duplexes 2 nmol each
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wdr5 mouse sirna oligo duplex  (OriGene)
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Wdr5 Mouse 3 unique 27mer siRNA duplexes 2 nmol each
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Image Search Results


Intracellular adenosine decreases the methylation level of H3K4. a Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs pretreated with 2 mM MTA for 30 min ( n = 4). b Western blot detection of methylation levels of H3K4, H3K9, and H3K27 in TNF-α (10 ng/ml for 12 h)-treated HUVECs pretreated with 100 µM adenosine for 30 min ( n = 5). c ChIP assay showing H3K4 methylation on promoters of adhesion molecules in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs ( n = 3). d Western blot detection of H3K4me2 in HUVEC whole-protein lysate supplemented with 1 mg/ml SAM or SAM together with 10 µM adenosine for 60 min ( n = 4). e Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or WDR5 siRNA ( n = 3). f Immunofluorescent ( IF ) staining of H3K4me2 on aortic endothelium (areas indicated with CD31 staining, red ) from TNF-α-treated ADK WT and ADK VEC-KO mice. L indicates luminal area of aorta ( scale bar , 100 µm). g Quantification of H3K4me2 on aortic endothelium from TNF-α-treated ADK WT and ADK VEC-KO mice ( n = 5 mice per group). h Western blot detection and densitometric quantification of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated MAECs isolated from ADK WT and ADK VEC-KO mice ( n = 4). i Western blot detection of H3K4 methylation in HUVECs. HUVECs, pretreated for 30 min with 10 µM NBMPR, were incubated with 100 µM adenosine for 30 min and then stimulated with TNF-α at 10 ng/ml for 4 h ( n = 3). j Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated A 2A R KD or Ctrl HUVECs pretreated with 100 µM adenosine for 30 min ( n = 4). k Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or A 2A R siRNA ( n = 4). For all bar graphs , data are the mean ± SEM, * P < 0.05 and ** P < 0.01 (unpaired, two-tailed Student’s t -test)

Journal: Nature Communications

Article Title: Regulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation

doi: 10.1038/s41467-017-00986-7

Figure Lengend Snippet: Intracellular adenosine decreases the methylation level of H3K4. a Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs pretreated with 2 mM MTA for 30 min ( n = 4). b Western blot detection of methylation levels of H3K4, H3K9, and H3K27 in TNF-α (10 ng/ml for 12 h)-treated HUVECs pretreated with 100 µM adenosine for 30 min ( n = 5). c ChIP assay showing H3K4 methylation on promoters of adhesion molecules in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs ( n = 3). d Western blot detection of H3K4me2 in HUVEC whole-protein lysate supplemented with 1 mg/ml SAM or SAM together with 10 µM adenosine for 60 min ( n = 4). e Western blot detection of adhesion molecules and H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or WDR5 siRNA ( n = 3). f Immunofluorescent ( IF ) staining of H3K4me2 on aortic endothelium (areas indicated with CD31 staining, red ) from TNF-α-treated ADK WT and ADK VEC-KO mice. L indicates luminal area of aorta ( scale bar , 100 µm). g Quantification of H3K4me2 on aortic endothelium from TNF-α-treated ADK WT and ADK VEC-KO mice ( n = 5 mice per group). h Western blot detection and densitometric quantification of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated MAECs isolated from ADK WT and ADK VEC-KO mice ( n = 4). i Western blot detection of H3K4 methylation in HUVECs. HUVECs, pretreated for 30 min with 10 µM NBMPR, were incubated with 100 µM adenosine for 30 min and then stimulated with TNF-α at 10 ng/ml for 4 h ( n = 3). j Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated A 2A R KD or Ctrl HUVECs pretreated with 100 µM adenosine for 30 min ( n = 4). k Western blot detection of H3K4 methylation in TNF-α (10 ng/ml for 4 h)-treated ADK KD or Ctrl HUVECs transiently transfected with control or A 2A R siRNA ( n = 4). For all bar graphs , data are the mean ± SEM, * P < 0.05 and ** P < 0.01 (unpaired, two-tailed Student’s t -test)

Article Snippet: The A 2A R (sc-39850), A 2B R (sc-29642), WDR5 (sc-61798) and the control (sc-37007) siRNAs were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA).

Techniques: Methylation, Western Blot, Transfection, Control, Staining, Isolation, Incubation, Two Tailed Test