Journal: mBio

Article Title: Sp1 facilitates continued HSV-1 gene expression in the absence of key viral transactivators

doi: 10.1128/mbio.03479-23

Figure Lengend Snippet: Antibodies for immunoblot experiments

Article Snippet: ICP0 , East Coast Bio , H1A027.

Techniques: Western Blot

Journal: Antiviral research

Article Title: CK2 Inhibitors Increase the Sensitivity of HSV-1 to Interferon-?

doi: 10.1016/j.antiviral.2011.06.009

Figure Lengend Snippet: The combination of CK2 inhibitor and IFN-β decreases early and late protein levels. HEL cells were treated and infected as described in Figure 1. At 12 and 24 hpi, cells were washed with PBS before being resuspended into loading buffer. Lysates were resolved by SDS-PAGE and analyzed by western blot for ICP4, UL42, VP5, or β-actin expression. Band intensities were measured by densitometry analyses using ImageJ. UL42 (ce):the UL42 24 hpi lanes contrast enhanced (ce) using Adobe Photoshop.

Article Snippet: 10% of each sample was resolved on a 4–12% Bis-tris polyacrylamide gel, transferred to nitrocellulose, blocked at room temperature for 1 h with 2% nonfat dry milk in Tris-buffered saline with 0.1% Tween 20 (TBS-T), and probed overnight at 4°C with antibodies directed against either ICP4 (H1A021, EastCoast), UL42 (mAB3678, Millipore), PML (A301-167A, Bethyl Laboratories), or β-actin ((I-19)-R, Santa Cruz Biotechnology).

Techniques: Infection, SDS Page, Western Blot, Expressing