Journal:

Article Title: Calmodulin protects cells from death under normal growth conditions and mitogenic starvation but plays a mediating role in cell death upon B-cell receptor stimulation

doi: 10.1046/j.1365-2567.2001.01259.x

Figure Lengend Snippet: Analysis of BCR induced cell cycle arrest (a) and cell death (b). Cells (1 × 106/ml) were stimulated with 4 µg/ml anti-sIgM antibody for 24 hr. (a) For cell cycle analysis cells were fixed in 70% ethanol/PBS and stained with propidium iodide prior to FACS analysis. The columns represent the mean (± SD, n = 3) percentage of cells in a particular cell cycle compartment (P < 0·005 wt, 8-3 and 8-7 M4 stimulated/non-stimulated in S and G2/M phases; P < 0·05 8-3/wt; 8-7/wt in G2 and M phases after M4 stimulation, all other differences between wt and CaMII ko cells after M4 stimulation were not significant (P > 0·05). (b) Cell death was analysed by staining the cells with merocyanine before FACS analysis. Death induction was calculated by subtracting the percentages of dead cells in the control from the stimulated populations. The columns represent the mean (± SD, n = 5) percentage of induced cell death * P < 0·005 (8-3/wt; 8-7/wt) by Student's t-test.

Article Snippet: His/neo resistant CaMII −/− clones were then checked for cell surface expression of the BCR immunoglobulin M (sIgM) by flow cytometry using a monoclonal anti-chicken sIgM antibody (Clone M4, CliniSciences, France). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Homologous recombination at the CaMII locus.

Techniques: Cell Cycle Assay, Staining