Journal: Pediatrics and neonatology

Article Title: Influence of prolonged storage process, pasteurization, and heat treatment on biologically-active human milk proteins.

doi: 10.1016/j.pedneo.2013.03.018

Figure Lengend Snippet: Figure 2 Concentrations of bioactive proteins in different forms of human milk. The concentrations of lactoferrin, lysozyme, secretory immunoglobulin A (sIgA), and leptin, in whey fractions isolated from freshly expressed human milk (fresh, n Z 16), prolonged frozen human milk (frozen, n Z 16) or pasteurized human milk (pasteurized, n Z 15) were analyzed as described in the Materials and methods section. *p < 0.0001 for fresh or frozen versus pasteurized milk; **p < 0.0001 for fresh versus frozen milk; ***p < 0.05 for fresh or frozen versus pasteurized milk.

Article Snippet: After appropriate preparation, enzyme-linked immunosorbent assay (ELISA) or the following enzyme immunoassay olonged Storage Process, Pasteurization, and Heat Treatment on 2013), http://dx.doi.org/10.1016/j.pedneo.2013.03.018 Figure 1 Description of the study design. kits were used to quantify the levels of sIgA, lysozyme, lactoferrin, and leptin of each milk sample: (1) sIgA ELISA kit (K8870; Immundiagnostik AG, Bensheim, Germany) with a sensitivity of 13.4 ng/mL, intra- and interassay coefficients of variation of 5e9% and 7.4e8.0%, respectively; (2) lysozyme enzyme immunoassay kit (Biomedical Technologies Inc., Stoughton, MA, USA) with a sensitivity of 0.78 ng/mL, intra- and interassay coefficients of variation of 5.3% and 7%, respectively; (3) lactoferrin enzyme immunoassay kit (Oxis International, Burlingame, CA, USA) with a sensitivity of 1.0 ng/mL, intra- and interassay coefficients of variation of 5.0% and 9.6%, respectively; (4) leptin ELISA kit (Millipore Corporation, Billerica, MA, USA) with a sensitivity of 0.5 ng/mL, intra- and interassay coefficients of variation of 2.6e4.6% and 2.6e6.2%, respectively.

Techniques: Isolation

Journal: Pediatrics and neonatology

Article Title: Influence of prolonged storage process, pasteurization, and heat treatment on biologically-active human milk proteins.

doi: 10.1016/j.pedneo.2013.03.018

Figure Lengend Snippet: Figure 3 Change in bioactive proteins after heat treatment. The concentrations of lactoferrin, lysozyme, secretory immuno- globulin A (sIgA), and leptin in whey fraction isolated from prolonged frozen human milk at 4C, 40C or 60C were assessed as described in the Materials and methods section. *p < 0.05 for 4C versus 40C or 60C; **p < 0.05 for 40C versus 60C.

Article Snippet: After appropriate preparation, enzyme-linked immunosorbent assay (ELISA) or the following enzyme immunoassay olonged Storage Process, Pasteurization, and Heat Treatment on 2013), http://dx.doi.org/10.1016/j.pedneo.2013.03.018 Figure 1 Description of the study design. kits were used to quantify the levels of sIgA, lysozyme, lactoferrin, and leptin of each milk sample: (1) sIgA ELISA kit (K8870; Immundiagnostik AG, Bensheim, Germany) with a sensitivity of 13.4 ng/mL, intra- and interassay coefficients of variation of 5e9% and 7.4e8.0%, respectively; (2) lysozyme enzyme immunoassay kit (Biomedical Technologies Inc., Stoughton, MA, USA) with a sensitivity of 0.78 ng/mL, intra- and interassay coefficients of variation of 5.3% and 7%, respectively; (3) lactoferrin enzyme immunoassay kit (Oxis International, Burlingame, CA, USA) with a sensitivity of 1.0 ng/mL, intra- and interassay coefficients of variation of 5.0% and 9.6%, respectively; (4) leptin ELISA kit (Millipore Corporation, Billerica, MA, USA) with a sensitivity of 0.5 ng/mL, intra- and interassay coefficients of variation of 2.6e4.6% and 2.6e6.2%, respectively.

Techniques: Isolation

Journal: Microorganisms

Article Title: Bacteriome Signature in SARS-CoV-2-Infected Patients Correlates with Increased Gut Permeability and Systemic Inflammatory Cytokines

doi: 10.3390/microorganisms13061407

Figure Lengend Snippet: Zonulin plasma concentrations ( A ), and fecal secretory IgA ( B ) in control subjects (CTL), acute COVID-19 patients, and post-COVID-19 condition (PCC) patients.

Article Snippet: Fecal secretory immunoglobulin A (sIgA) was quantified by the commercial Human IgA ELISA kit (Elabscience, Bethesda, MD, USA), according to the manufacturer’s protocol.

Techniques: Clinical Proteomics, Control

Journal: Microorganisms

Article Title: Bacteriome Signature in SARS-CoV-2-Infected Patients Correlates with Increased Gut Permeability and Systemic Inflammatory Cytokines

doi: 10.3390/microorganisms13061407

Figure Lengend Snippet: Correlations between taxonomic counts of the gut bacteriome and clinical and laboratory data in COVID-19 patients. ( A ) C-reactive protein with differentially increased genera. ( B , C ) Plasma zonulin levels with differentially increased families and genera. ( D , E ) Fecal sIgA levels with differentially increased Bacillota, Prevotellacea, and some genera. ( F – H ) Plasma concentrations of IL-6, IL-2 and IFN-γ with differentially increased taxa.

Article Snippet: Fecal secretory immunoglobulin A (sIgA) was quantified by the commercial Human IgA ELISA kit (Elabscience, Bethesda, MD, USA), according to the manufacturer’s protocol.

Techniques: Clinical Proteomics

Journal: Poultry science

Article Title: Effects of Paenibacillus xylanexedens on growth performance, intestinal histomorphology, intestinal microflora, and immune response in broiler chickens challenged with Escherichia coli K88.

doi: 10.3382/ps/pez460

Figure Lengend Snippet: Figure 4. Effects of Paenibacillus xylanexedens ysm1 on concentra- tion of sIgA in the jejunum of broilers. Each bar represents mean ± SE values of 8 replicates per treatment. Within the same day, bars with different letters (a-d) differ significantly (P < 0.001)

Article Snippet: Secretory IgA (sIgA) ELISA Kit (Elabscience Biotechnology Co., Wuhan, PRC) was used in accordance with the manufacturer’s instructions for sIgA concentration on days 14 and 28.

Techniques: