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Siemens AG
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OmicsLink shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA
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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Inhibition of 6-phosphogluconate Dehydrogenase Reverses Cisplatin Resistance in Ovarian and Lung Cancer
doi: 10.3389/fphar.2017.00421
Figure Lengend Snippet: MiR-206 and miR-613 directly inhibit the expression of 6PGD through its 3′UTR. (A,B) qRT-PCR analysis of miR-206 and miR-613 expression in OV2008 and C13 ∗ cells (A) and A549 and A549DDP cells (B) . (C,D) The effects of miRNAs on 6PGD mRNA, protein expression and enzyme activity, were determined at 48 h after transfected with 100 nM Mimic Negative control (Mimic NC) and 100 nM miR-206 and miR-613 in C13 ∗ (C) and A549DDP (D) cells, respectively. (E,F) The effects of miRNA inhibitors on 6PGD mRNA, protein expression and enzyme activity, were determined at 48 h after transfected with Inhibitor negative control (Inhibitor NC) and miR-206 inhibitor and miR-613 inhibitor in OV2008 cells (E) and A549 cells (F) , respectively. (G) 293T cells were co-transfected with Renilla luciferase plasmid and a firefly luciferase reporter plasmid containing either wild-type or mutant 6PGD 3′UTR (indicated as pGL3-6PGD-3′UTR-wt and pGL3-6PGD-3′UTR-mu) with either control or miR-206 or miR-613. Luciferase activity was conducted at 24 h after transfection. Error bars represent mean values ± SD from three replicates of each sample ( ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001).
Article Snippet: The miR-206, miR-613 and
Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Transfection, Negative Control, Luciferase, Plasmid Preparation, Mutagenesis, Control
Journal: Frontiers in Pharmacology
Article Title: Inhibition of 6-phosphogluconate Dehydrogenase Reverses Cisplatin Resistance in Ovarian and Lung Cancer
doi: 10.3389/fphar.2017.00421
Figure Lengend Snippet: miR-206 and miR-613 have rewired cellular metabolism by targeting 6PGD. (A,B) C13 ∗ (A) and A549DDP (B) cell lines were transfected with Mimic NC, miR-206 and miR-613, respectively. The effects of miRNAs on NADPH/NADP + ratio, lactate production and intracellular ATP levels were determined. (C,D) OV2008 (C) and A549 (D) cell lines were transfected with Inhibitor NC and miR-206 inhibitor and miR-613 inhibitor, respectively. The effects of miRNA inhibitors on NADPH/NADP + ratio, lactate production and intracellular ATP levels were determined. Error bars represent mean values ± SD from three replicates of each sample ( ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001).
Article Snippet: The miR-206, miR-613 and
Techniques: Transfection