Journal: Developmental cell

Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer

doi: 10.1016/j.devcel.2017.10.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cross) N/A Experimental Models: Organisms/Strains Mouse: SCID Beige (CB17.Cg-Prkdc scid Lyst bg-J /Crl) Charles River Strain #250, RRID: IMSR_CRL:250 Mouse: BALB/c (BALB/cAnNCrl) Charles River Strain #028, RRID: IMSR_CRL:28 Oligonucleotides Primers for quantitative PCR, see Table S6 This paper N/A Recombinant DNA TET-pLKO.1 puro shLuc This paper Addgene #83092 TET-pLKO.1 puro shGFP This paper Addgene #83085 TET-pLKO.1 puro shLacZ This paper Addgene #98632 TET-pLKO.1 puro shGDF11 #1 This paper Addgene #83083 TET-pLKO.1 puro shGDF11 #2 This paper Addgene #83084 TET-pLKO.1 puro shSMAD4 #1 This paper Addgene #83089 TET-pLKO.1 puro shSMAD4 #2 This paper Addgene #83091 TET-pLKO.1 puro shID2 #1 This paper Addgene #83086 TET-pLKO.1 puro shID2 #2 This paper Addgene #83087 TET-pLKO.1 puro shId2 #1 This paper Addgene #83088 TET-pLKO.1 puro shId2 #2 This paper Addgene #83090 pLX304 LacZ-2′V5 blast This paper Addgene #83099 pLX304 GDF11-V5 blast This paper Addgene #83098 pLX304 PCSK5-V5 blast This paper Addgene #83100 pLX304 PCSK5 (T288P)-V5 blast This paper Addgene #83101 pLX304 PCSK5 (A270T)-V5 blast This paper Addgene #83102 pLX304 PCSK5 (H516P)-V5 blast This paper Addgene #83103 pLX304 PCSK5 (R511C)-V5 blast This paper Addgene #83104 pLX304 Luciferase-V5 blast This paper Addgene #98580 pLX302 ID2-V5 puro This paper Addgene #83096 pLX302 GDF11-V5 puro This paper Addgene #83097 pLX302 PCSK5-V5 puro This paper Addgene #98389 pLX302 PCSK5 (T288P)-V5 puro This paper Addgene #98709 pLX302 LacZ-2′V5 blast This paper Addgene #98579 pEN_TT_miRc2 3xFLAG This paper Addgene #83274 pEN_TT_miRc2 3xFLAG LacZ This paper Addgene #83093 pEN_TT_miRc2 3xFLAG Luciferase This paper Addgene #98391 pEN_TT_miRc2 3XFLAG SMAD4 This paper Addgene #83094 pEN_TT_miRc2 3XFLAG ID2 This paper Addgene #98390 pSLIK_TT 3xFLAG LacZ neo This paper Addgene #83105 pSLIK_TT 3xFLAG LacZ hygro This paper Addgene #98395 pSLIK_TT 3xFLAG Luciferase neo This paper Addgene #98392 pSLIK_TT 3xFLAG SMAD4 neo This paper Addgene #83273 pSLIK_TT 3xFLAG ID2 neo This paper Addgene #98393 pSLIK_TT 3xFLAG ID2 hygro This paper Addgene #98394 pBabe TGFBR3-HA neo This paper Addgene #83095 pBabe Venus neo This paper Addgene #98396 pBabe mRFP1 neo This paper Addgene #98397 pLKO.1 shTGFBR3 puro Wang et al., 2014 .

Techniques: In Situ, Recombinant, Enzyme-linked Immunosorbent Assay, Mutagenesis, Amplification, Expressing, Real-time Polymerase Chain Reaction, Luciferase, Software

Journal: PLoS Pathogens

Article Title: The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes

doi: 10.1371/journal.ppat.1005181

Figure Lengend Snippet: (A) Northern blot of RNA isolated from CIN612 cells stably selected to express control shRNA and shSIRT1 constructs. (B) Sub-saturated X-ray films from (A) were scanned and analyzed by Licor signal intensity analysis and expressed as fold change of late transcripts E1^E4 and E5 relative to CIN612 undifferentiated control. Error bars are +/- 1 SEM. (C) HFKs and HFKs stably expressing HPV31 and either shGFP or shSIRT1 were grown in organotypic raft culture for 14 days. HE is hematoxylin/eosin stain. E1E4 is an HPV31 late gene product and is expressed in upper layers of infected epithelium. K10 is a differentiation marker.

Article Snippet: Stocks were grown and plasmid isolated as per the manufacturer instructions. shGFP plasmid was purchased from Addgene (plasmid 30323 from David Sabattini) [ ].

Techniques: Northern Blot, Isolation, Stable Transfection, Control, shRNA, Construct, Expressing, Staining, Infection, Marker

Journal: bioRxiv

Article Title: RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of α 2 GABA A receptors

doi: 10.1101/197905

Figure Lengend Snippet: Morphology of HEK-293T cells transfected with eGFP-CB2ΔPH, eGFP-CB1 SH3- or eGFP-CB2 SH3- and stained for actin using phalloidin. (D) Quantification of filopodia in HEK-293T cells expressing eGFP, eGFP-CB1 SH3+ , eGFP-CB1 SH3- , eGFP-CB2 SH3+ or eGFP-CB2 SH3- shows an increase in cells expressing CB isoforms. (E-G) HEK-293T cell morphology after transfection with eGFP, eGFP and VSVG-Cdc42 (CA) or VSVGCdc42 (DN). Arrow heads show accumulation of VSVG-Cdc42 (DN) (red) in filopodia. (H-I) HEK-293T cells co-transfected with VSVG-Cdc42 (CA) and eGFP-CB1 SH3- or eGFP-CB2 SH3- . Arrow heads show accumulation of VSVG-Cdc42 (CA) (red) in filopodia. (H’-I’) HEK-293T cell morphology after co-transfection with VSVG-Cdc42 (DN) and eGFP-CB1 SH3- or eGFP-CB2 SH3- showing enhanced filopodia formation. Arrow head show accumulation of VSVG-Cdc42 (DN) (red) in filopodia. (J-J’) HEK-293T cell morphology after co-transfection with mCherry-gephyrin and eGFP-CB1 SH3- or eGFP-CB2 SH3- . Arrow show membrane ruffling instead of filopodia. (K) Quantification of filopodia in HEK-293T cells upon co-expression of VSVG-Cdc42CA, VSVG-Cdc42DN or mCherry-gephyrin along with V5-CB isoforms.

Article Snippet: FLAG-gephyrin is described previously (Tyagarajan et al 2011). pGEX-2T-GST Cdc42WT was obtained from Addgene (#12969) and pGEXTK-Pak1 (70-117) was obtained from Addgene (#12117). pRKVSV-Cdc42 WT, pRKVSV-Cdc42 DN (N17), pRKVSV-Cdc42 CA (QL) were gifts from Prof. Kenneth Yamada (NIH).

Techniques: Transfection, Staining, Expressing, Cotransfection

Journal: bioRxiv

Article Title: RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of α 2 GABA A receptors

doi: 10.1101/197905

Figure Lengend Snippet: Adult newborn neurons expressing eGFP-Cdc42 (DN) do not show any migration defects at 14 dpi, 28 dpi and 42 dpi. (B-B”) Adult newborn neurons expressing eGFP-Cdc42 (DN) show defect in dendrite maturation at 14 dpi, 28 dpi and 42 dpi. (C-C’) Adult newborn neurons expressing eGFP-CB1 SH3- 2R mutant exhibit reduced dendritic complexity at 28 dpi, but not 42 dpi. (D) Morphology of adult newborn neuron expressing eGFP, eGFPCdc42 (DN) or eGFP-CB1 SH3- 2R mutant. (E) Quantification of total dendrite length showing reduced length in neurons expressing eGFP-CB1 SH3- 2R mutant at 28 dpi, but not 42 dpi. (F) The number of terminal dendrites is reduced in neurons expressing eGFPCB1 SH3- 2R mutant at both 28 dpi and 42 dpi. Kolmogorov-Smirnov test (A-A”), N = 38-51 cells/group. Mann-Whitney t-test AUC (B-B”, C-C’), N = 12-41 cells/group. Two-way ANOVA, Bonferroni post-hoc test (E,F), N = 12-25 cells/group.

Article Snippet: FLAG-gephyrin is described previously (Tyagarajan et al 2011). pGEX-2T-GST Cdc42WT was obtained from Addgene (#12969) and pGEXTK-Pak1 (70-117) was obtained from Addgene (#12117). pRKVSV-Cdc42 WT, pRKVSV-Cdc42 DN (N17), pRKVSV-Cdc42 CA (QL) were gifts from Prof. Kenneth Yamada (NIH).

Techniques: Expressing, Migration, Mutagenesis, MANN-WHITNEY