Journal: Developmental cell

Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer

doi: 10.1016/j.devcel.2017.10.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TET-pLKO.1 puro shGFP , This paper , Addgene #83085.

Techniques: Virus, In Situ, Recombinant, Enzyme-linked Immunosorbent Assay, Mutagenesis, RNA Amplification, Expressing, Real-time Polymerase Chain Reaction, Luciferase, Software

Journal: Nature genetics

Article Title: PGBD5 promotes site-specific oncogenic mutations in human tumors

doi: 10.1038/ng.3866

Figure Lengend Snippet: (a) Tumor volume of RPE cells as a function of time in primary (light gray box) and secondary (dark gray box) transplants, with PGBD5 expression induced using doxycycline (black), as indicated. RPE cells were treated with doxycycline in vitro for 10 days prior to transplantation. Arrowhead denotes withdrawal of doxycycline from the diet (red). Inset: Western blot of PGBD5 protein, as compared to actin control in cells derived from tumors after primary transplant. (b) Representative photomicrographs of hematoxylin and eosin stained tumor sections from doxycycline-inducible PGBD5 -expressing RPE tumors after continuous (+Dox) and discontinuous (-Dox) doxycycline treatment. (c) Western blot of PGBD5 in G401 and A204 rhabdoid tumor cells upon depletion of PGBD5 using two independent shRNAs, as compared to non-transduced cells and control cells expressing shGFP. (d) Relative number of viable G401 and A204 cells upon 72 hours of PGBD5 shRNA depletion. Errors bars represent standard deviations of 3 independent experiments.

Article Snippet: Lentiviral shRNA and doxycycline-inducible WWOX expression vectors were a kind gift of Marcelo Aldaz . pLKO.1 shRNA vectors targeting PGBD5 (TRCN0000138412, TRCN0000135121) and control shGFP were obtained from the RNAi Consortium (Broad Institute, Cambridge, MA).

Techniques: Expressing, In Vitro, Transplantation Assay, Western Blot, Control, Derivative Assay, Staining, shRNA