Journal: International Journal of Molecular Sciences

Article Title: Maternal Immune Activation Induces Neuroinflammation and Cortical Synaptic Deficits in the Adolescent Rat Offspring

doi: 10.3390/ijms21114097

Figure Lengend Snippet: MIA reduces the level of synaptic scaffold proteins—SHANK. Effects of MIA on post-synaptic SHANK protein family members were analyzed in the cerebral cortex of PND 53–54 rats. Immunoreactivities of SHANK1 ( a ), SHANK2 ( c ), SHANK3 ( e ), and GAPDH were analyzed by SDS-PAGE and western blot with representative blots presented. The mRNA levels of Shank1-3 ( b , d , f ) were determined using real-time PCR normalized to Actb (β-actin). Data represent the mean value ± S.E.M. (a–n = 3, b–n = 5–6, c–n = 3–4, d–n = 6–5, e–n = 3–4, f–n = 4). * p < 0.05, ** p < 0.01 compared to control group using Student’s t -test.

Article Snippet: Expression levels of mRNA were measured with real-time PCR, using the TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA): Vamp1 (Rn 00565308_m1), Vamp2 (Rn 00360268_g1), Stx1a (Rn 00587278_m1), Stx1b (Rn 01510167-m1), Snap25 (Rn 00578534_m1), Syt1 (Rn 00436862_m1), Syp (Rn 01528256_m1), Shank1 (Rn 00582088_m1), Shank2 (Rn 01479040_m1), Shank3 (Rn 00572344_m1), Il6 (Rn 01410339-m1), Tnf (Rn 01525859_g1), Alox12 (Rn 01461081_m1), Ptgs2 (Rn 01483828-m1) and Actb ( Rn 00667869_m1) as the reference gene, on an ABI PRISM 7500 apparatus.

Techniques: SDS Page, Western Blot, Real-time Polymerase Chain Reaction, Control