Journal: The Journal of allergy and clinical immunology

Article Title: Estrogen increases the severity of anaphylaxis in female mice through enhanced eNOS expression and NO production

doi: 10.1016/j.jaci.2014.11.003

Figure Lengend Snippet: (A) Temperature changes in female and male C57Bl/6 mice during anaphylaxis. Mice were sensitized with DNP-specific IgE and challenged 24 h later with DNP-HSA. Data are from 3 independent experiments of n=6/group. (B–D) Temperature changes during anaphylaxis in ovariectomized (OVX) or sham-operated female mice (B), after implantation in OVX mice of 17β-estradiol-releasing (OVX+E2) or placebo pellets (OVX) (C), and in females after treatment with the estrogen receptor antagonist ICI182,780 (ER-ant) (D). In B–D, n=6 mice/group. *** P<0.001.

Article Snippet: Mice Wild type (WT) C57Bl/6, Balb/c and NOS3 −/− mice, as well as ovariectomized (OVX) and sham-operated mice were obtained from Jackson Laboratory (Bar Harbor, ME).

Techniques:

Journal: The Journal of allergy and clinical immunology

Article Title: Estrogen increases the severity of anaphylaxis in female mice through enhanced eNOS expression and NO production

doi: 10.1016/j.jaci.2014.11.003

Figure Lengend Snippet: (A, B) Expression levels of eNOS and Ser1177-phosphorylated eNOS (p-eNOS) in female (F), male (M) and OVX female lung lysates at baseline and after IgE/Ag-induced PSA. In A, representative Western blots of lung lysates are shown. Numbers below indicate the fold increases in eNOS or p-eNOS band intensities (corrected to their respective β-actin loading control) as compared to males. In B the band intensitites in lung eNOS and p-eNOS (n=5/group) are shown. *P<0.05, **P<0.01,*** P<0.001. No statistical differences were found in the levels of eNOS or p-eNOS between males and OVX females.

Article Snippet: Mice Wild type (WT) C57Bl/6, Balb/c and NOS3 −/− mice, as well as ovariectomized (OVX) and sham-operated mice were obtained from Jackson Laboratory (Bar Harbor, ME).

Techniques: Expressing, Western Blot, Control