Journal: Cell Reports Medicine

Article Title: Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma

doi: 10.1016/j.xcrm.2024.101791

Figure Lengend Snippet: Figure 1. sFRP1 was abundantly produced in nerve ECM following injury and associated with nerve degeneration (A) The isolation of sciatic nerve samples and proteomic analysis process. (B) The clustering distribution of injured and uninjured nerve samples as plotted by PCA analysis. (C) Differentially expressed proteins between injured and uninjured nerve samples are displayed in volcano plot. N = 3 mice. Proteins regulated over 1.5-fold changes (adj. p < 0.05) are highlighted in blue (downregulated) and red (upregulated). (D) GO enrichment analysis indicating the classification of differentially expressed proteins related to the biological process category. (E) Differentially expressed proteins in the GO category of ECM are displayed as a heatmap. (F) Western blotting analysis demonstrating increased production of sFRP1 in the injured nerve tissue. (G) Quantification of sFRP1 protein level in sciatic nerves isolated from uninjured and injured mice as indicated by western blot analysis. N = 3 mice. (H) Representative TEM, HE, and TB images of injured nerves isolated from mice treated with WAY-316606 and PBS at 3 weeks post injury. N = 6 mice. (I and J) Quantification of myelinated axon diameter and g-ratio as indicated in TEM images. (K) Quantification of myelinated axon density as indicated in HE-stained images. (L) Representative IHC images of human nerves stained for sFRP1 at 12 h after injury. Statistical significance was determined using two-tailed unpaired Student’s t tests; **p < 0.01; *p < 0.05; ns, no difference. Data were presented as mean ± SD.

Article Snippet: For recombinant sFRP1 protein treatment, mice were intraneurally injected with sFRP1 protein (HY-P73413, MedChemExpress, 500nM, 5mL) or PBS (5mL), immediately after nerve transection procedure.

Techniques: Produced, Isolation, Western Blot, Staining, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma

doi: 10.1016/j.xcrm.2024.101791

Figure Lengend Snippet: Figure 3. Mice with deletion of sFRP1 in SCs profoundly reduced macrophage infiltration and improved nerve regeneration (A) Sfrp1flox/flox mice were bred with PlpcreErt1 mice to generate tamoxifen-inducible SC-specific sFRP1 knockout (Sfrp1flox/floxPlpcreErt1) and littermate control (Sfrp1flox/flox) mice. (B and C) Representative SCG10 immunostaining and related quantification of sciatic nerves at 14 days post transection. N = 6 mice. The dashed line indicates the transection site. Scale bar, 500 mm. (D and E) Representative F4/80 immunostaining (red) of sciatic nerves taken from the injury site, 1,000, 2,000, and 3,000 mm distal to the injury site and related quantification of infiltrated macrophages. Scale bar, 100 mm. N = 6 mice. (F and G) Western blot analysis and related quantification of TNF-a level in injured nerves at 24 h post transection. N = 3 mice. (H and I) Triple staining of CCL2 (green), F4/80 (red), and NeuN (pink) on sciatic DRG sections from Sfrp1flox/flox and Sfrp1flox/floxPlpcreErt1 mice and related quantification of CCL expression level in DRGs. N = 6 mice. No significant difference of CCL2 expression is observed between DRGs of Sfrp1flox/flox and Sfrp1flox/floxPlpcreErt1 mice. (J–L) Representative TUBB3 immunostaining (green) of sciatic DRG neurons isolated from Sfrp1flox/flox and Sfrp1flox/floxPlpcreErt1 mice (n = 6 mice) and related quantification. DRG neurons were cultured in vitro for 4 days or 7 days. (M and N) Representative immunostaining and related quantification of ATF3 (red) and the neuronal marker NeuN (green) in sciatic DRGs at 24 h after nerve injury. N = 6 mice. Scale bar, 50 mm. Statistical significance in (C) and (E) was analyzed by two-way ANOVA followed by Sidak’s post hoc analysis. Statistical significance was determined using two- tailed unpaired Student’s t tests; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, no significance. Data were presented as mean ± SD.

Article Snippet: For recombinant sFRP1 protein treatment, mice were intraneurally injected with sFRP1 protein (HY-P73413, MedChemExpress, 500nM, 5mL) or PBS (5mL), immediately after nerve transection procedure.

Techniques: Knock-Out, Control, Immunostaining, Western Blot, Staining, Expressing, Isolation, Cell Culture, In Vitro, Marker, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma

doi: 10.1016/j.xcrm.2024.101791

Figure Lengend Snippet: Figure 4. SFRP1 induces the F4/80+ CD86+ proinflammatory macrophage phenotype and inhibits oxidative metabolism (A and B) The axon length of sciatic DRG neurons demonstrates no significant difference in response to sFRP1 treatment. N = 6 biological replicates. (C) Representative TEM images reveal that the morphology and structure of mitochondria were well preserved in sFRP1-treated neurons. (D and E) Representative TEM images and related quantification of nerve transections (N = 6 mice). The suppressing effect of sFRP1 on axon regrowth is alleviated in a macrophage-deficient condition. (F) Double staining of IL-1b (red) and TNF-a (green) on sFRP1-treated BMDMs. (G) sFRP1-induced phenotypic switch is revealed by flow cytometric quantification. FITC reflects F4/80-positive cells. PE reflects CD206-positive cells. APC reflects CD86-positive cells. (H and I) Quantification of the percentage of IL-1b and TNF-a-positive cells as reflected by Figure 4F. Biological replicates n = 3 with two technical replicates each. (J) Double staining of Arg-1 (red) and Wnt3a (green) on sFRP1 and PBS-treated BMDMs. (K) The internalizing capacity of BMDMs was measured by incubating with 100 mg/mL pHrodo BioParticles (green). BMDMs were visualized by F4/80 (red) staining.

Article Snippet: For recombinant sFRP1 protein treatment, mice were intraneurally injected with sFRP1 protein (HY-P73413, MedChemExpress, 500nM, 5mL) or PBS (5mL), immediately after nerve transection procedure.

Techniques: Double Staining, Staining

Journal: Cell Reports Medicine

Article Title: Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma

doi: 10.1016/j.xcrm.2024.101791

Figure Lengend Snippet: Figure 6. Depletion of HSP90 in macrophages attenuated neuroinflammation and nerve degenerative changes exerted by sFRP1 (A) Hsp90aaflox/+ mice were bred with Lyz2-cre mice to generate macrophage-specific HSP90-deficient (Hsp90aaflox/+Lyz2-cre) and littermate control (Hsp90aaflox/+) mice. (B and C) Representative IF images of SCG10 staining and related quantification of sciatic nerves at 2 weeks post injury. The dashed line indicates the transection site. Scale bar, 500 mm. N = 6 mice. (D and E) Representative IF images of F4/80 staining (red) of sciatic nerves and related quantification of macrophages at 2 weeks post injury. Scale bar, 100 mm. N = 6 mice. (F–I) Double staining of TNF-a (red) and IL-1b (green) on nerve longitudinal sections and related quantification. (J–L) Representative TUBB3 staining (green) and related quantification of sciatic DRG neurons isolated from Hsp90aaflox/+ and Hsp90aaflox/+Lyz2-cre mice after 4 days and 7 days of culture. Biological replicates n = 3 with two technical replicates each. Statistical significance was determined using two-way ANOVA followed by Sidak’s post hoc analysis in (C) and (E), and using two-tailed unpaired Student’s t tests in (F), (G), (K), and (L); **p < 0.01; ***p < 0.001; *p < 0.05; ns, no significance. Data were presented as mean ± SD.

Article Snippet: For recombinant sFRP1 protein treatment, mice were intraneurally injected with sFRP1 protein (HY-P73413, MedChemExpress, 500nM, 5mL) or PBS (5mL), immediately after nerve transection procedure.

Techniques: Control, Staining, Double Staining, Isolation, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma

doi: 10.1016/j.xcrm.2024.101791

Figure Lengend Snippet: Figure 7. SFRP1-neutralizing antibody treatment improved axon regeneration in vivo and in vitro (A and B) Representative SCG10 immunostaining and related quantification of murine injured nerves at 2 weeks after nerve transection. The dashed line indicates the transection site. Scale bar, 500 mm. N = 6 mice. (C) Schematic diagram of DRG neuron and macrophage microfluidic coculture chamber assay. (D) Representative optical images of macrophages in the neuron-macrophage coculture chambers. (E and F) Representative TUBB3 immunofluorescent images of neurons in the neuron-macrophage co-culture chambers and related quantification of average axon length in microfluidic channels. Biological replicates n = 3 with two technical replicates each. (G) Schematic diagram of DRG neuron and macrophage direct coculture assay. (H and I) Representative IF images stained for TUBB3 (green) on sciatic DRG neurons, and quantification of average axon length per cell in the direct coculture dishes. Biological replicates n = 3 with two technical replicates each. Statistical significance was determined using two-way ANOVA followed by Sidak’s post hoc analysis in (B) and (I) and using two-tailed unpaired Student’s t tests in (F); ***p < 0.001; **p < 0.01; *p < 0.05. Data were presented as mean ± SD.

Article Snippet: For recombinant sFRP1 protein treatment, mice were intraneurally injected with sFRP1 protein (HY-P73413, MedChemExpress, 500nM, 5mL) or PBS (5mL), immediately after nerve transection procedure.

Techniques: In Vivo, In Vitro, Immunostaining, Boyden Chamber Assay, Co-Culture Assay, Co-culture Assay, Staining, Two Tailed Test

Journal: Molecular Medicine

Article Title: Ginsenoside Rb1 affects mitochondrial Ca 2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1

doi: 10.1186/s10020-024-01009-0

Figure Lengend Snippet: Screening of the optimal ginsenoside Rb1 concentration. A . IF staining for α-SMA. B . Cell proliferation was assessed using the CCK-8 assay. C and D . The levels of the Collagen II, FN and α-SMA mRNAs and proteins were detected via qRT‒PCR and Western blot. * P < 0.05 compared with the Normal group and # P < 0.05 compared with the Control group

Article Snippet: The primary antibodies used included Collagen II (AWA01318, 1:1000, Abiowell), FN (AWA00143, 1:1000, Abiowell), α-SMA (AWA00774, 1:1000, Abiowell), SFRP1 (AWA01318, 1:1000, Abiowell), CaMKII (AWA51690, 1:1000, Abiowell), Caspase-3 (AWA45560, 1:1000, Abiowell), Bax (AWA00856, 1:1000, Abiowell), Bcl-2 (AWA00387, 1:1000, Abiowell), Pparγ (16643-1-AP, 1:1000, Proteintech), C/ebpα (29388-1-AP, 1:1000, Proteintech), Fas (ab271016, 1:1000, Abcam), Fabp4 (12802-1-AP, 1:5000, Proteintech), Apn (14553-1-AP, 1:1000, Proteintech), and β-actin (AWA80002, 1:5000, Abiowell).

Techniques: Concentration Assay, Staining, CCK-8 Assay, Western Blot, Control

Journal: Molecular Medicine

Article Title: Ginsenoside Rb1 affects mitochondrial Ca 2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1

doi: 10.1186/s10020-024-01009-0

Figure Lengend Snippet: Ginsenoside Rb1 improved RCTs in rats. A . HE staining of the injured tissue from each group. B . Masson’s trichrome staining of infiltrated tissue fibers. C . Oil red O staining of fat accumulation in the tissue. D . qRT‒PCR and Western blot were performed to detect the expression of lipid-related molecules (Pparγ, C/ebpα, Fas, Fabp4, and Apn). E . Collagen II, FN and α-SMA mRNA levels. F . Protein expression of Collagen II, FN, α-SMA and SFRP1. G . IF staining showing SFRP1 levels. * P < 0.05 compared with the Sham group and # P < 0.05 compared with the RCTs group

Article Snippet: The primary antibodies used included Collagen II (AWA01318, 1:1000, Abiowell), FN (AWA00143, 1:1000, Abiowell), α-SMA (AWA00774, 1:1000, Abiowell), SFRP1 (AWA01318, 1:1000, Abiowell), CaMKII (AWA51690, 1:1000, Abiowell), Caspase-3 (AWA45560, 1:1000, Abiowell), Bax (AWA00856, 1:1000, Abiowell), Bcl-2 (AWA00387, 1:1000, Abiowell), Pparγ (16643-1-AP, 1:1000, Proteintech), C/ebpα (29388-1-AP, 1:1000, Proteintech), Fas (ab271016, 1:1000, Abcam), Fabp4 (12802-1-AP, 1:5000, Proteintech), Apn (14553-1-AP, 1:1000, Proteintech), and β-actin (AWA80002, 1:5000, Abiowell).

Techniques: Staining, Western Blot, Expressing

Journal: Molecular Medicine

Article Title: Ginsenoside Rb1 affects mitochondrial Ca 2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1

doi: 10.1186/s10020-024-01009-0

Figure Lengend Snippet: Ginsenoside Rb1 improved RCTs by influencing SFRP1 to inhibit fat deposition and fibrosis. A . HE staining of the injured tissue from each group. B . Masson staining of infiltrated tissue fibers. C . Oil red O staining of fat accumulation in the tissue. D . qRT‒PCR and Western blot were utilized to measure the expression of lipid-related molecules (Pparγ, C/ebpα, Fas, Fabp4, and Apn). E . Collagen II, FN, α-SMA and SFRP1 mRNA and protein expression. F . IF staining showing Collagen II, FN, and α-SMA levels. * P < 0.05 compared with the Sham group, # P < 0.05 compared with the RCTs group, @ P < 0.05 compared with the Rb1 + oe-NC group, and & P < 0.05 compared with the Rb1 + si-NC group

Article Snippet: The primary antibodies used included Collagen II (AWA01318, 1:1000, Abiowell), FN (AWA00143, 1:1000, Abiowell), α-SMA (AWA00774, 1:1000, Abiowell), SFRP1 (AWA01318, 1:1000, Abiowell), CaMKII (AWA51690, 1:1000, Abiowell), Caspase-3 (AWA45560, 1:1000, Abiowell), Bax (AWA00856, 1:1000, Abiowell), Bcl-2 (AWA00387, 1:1000, Abiowell), Pparγ (16643-1-AP, 1:1000, Proteintech), C/ebpα (29388-1-AP, 1:1000, Proteintech), Fas (ab271016, 1:1000, Abcam), Fabp4 (12802-1-AP, 1:5000, Proteintech), Apn (14553-1-AP, 1:1000, Proteintech), and β-actin (AWA80002, 1:5000, Abiowell).

Techniques: Staining, Western Blot, Expressing

Journal: Molecular Medicine

Article Title: Ginsenoside Rb1 affects mitochondrial Ca 2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1

doi: 10.1186/s10020-024-01009-0

Figure Lengend Snippet: Ginsenoside Rb1 regulated the Wnt signaling pathway through SFRP1 to affect mitochondrial Ca 2+ transport and inhibit cell damage and fibrosis. A . The CCK-8 assay was performed to detect cell proliferation in each group. B . Apoptosis was determined by flow cytometry. C . Western blot analysis of Caspase-3, Bax, and Bcl-2 levels. D . Western blot analysis of Collagen II, FN and α-SMA levels in cells. E . The contents of TNF-α, IL-1β and IL-6. * P < 0.05 compared with the Control group, # P < 0.05 compared with the Rb1 + oe-NC group, and & P < 0.05 compared with the Rb1 + si-NC group

Article Snippet: The primary antibodies used included Collagen II (AWA01318, 1:1000, Abiowell), FN (AWA00143, 1:1000, Abiowell), α-SMA (AWA00774, 1:1000, Abiowell), SFRP1 (AWA01318, 1:1000, Abiowell), CaMKII (AWA51690, 1:1000, Abiowell), Caspase-3 (AWA45560, 1:1000, Abiowell), Bax (AWA00856, 1:1000, Abiowell), Bcl-2 (AWA00387, 1:1000, Abiowell), Pparγ (16643-1-AP, 1:1000, Proteintech), C/ebpα (29388-1-AP, 1:1000, Proteintech), Fas (ab271016, 1:1000, Abcam), Fabp4 (12802-1-AP, 1:5000, Proteintech), Apn (14553-1-AP, 1:1000, Proteintech), and β-actin (AWA80002, 1:5000, Abiowell).

Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Long-Term Estrogen Receptor Beta Agonist Treatment Modifies the Hippocampal Transcriptome in Middle-Aged Ovariectomized Rats

doi: 10.3389/fncel.2016.00149

Figure Lengend Snippet: PCR Results .

Article Snippet: Rn01478472_m1 , 2.777 , 0.088 , Sfrp1 , Secreted frizzled-related protein 1.

Techniques: Binding Assay