Journal: Clinica chimica acta; international journal of clinical chemistry

Article Title: Total testosterone quantitative measurement in serum by LC-MS/MS ☆

doi: 10.1016/j.cca.2014.06.009

Figure Lengend Snippet: Accuracy traceability to JCTLM listed materials and NIST serum reference materials.

Article Snippet: Serum-based reference material SRM 971 at 2 concentrations was obtained from NIST, and ERM-DA345 and ERM-DA346 from LGC Standards for accuracy assessment.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: HC diet inhibited testosterone synthesis by activating endoplasmic reticulum stress in testicular Leydig cells

doi: 10.1111/jcmm.14143

Figure Lengend Snippet: The high‐cholesterol (HC) diet increases serum cholesterol level and decreases testosterone level in rats. Rats were treated with a normal control diet (NC group) or a HC diet (HC group) for 16 wk. During the feeding period, the bodyweight of these rats was monitored weekly (A), and the serum total cholesterol (D) and testosterone (F) levels of the rats were assayed every 4 wk. In addition, testicular weight (B), epididymal fat weight (C), serum triglyceride (E), intratesticular testosterone (G) and LH (H) levels were detected at the 16th wk. Data points are presented as the mean ±SEM (n = 8 per group). # P < 0.05 vs the NC group

Article Snippet: Serum total testosterone, intratesticular testosterone (Uscn Life Science & Technology Co., Ltd., Wuhan, China) and serum LH (LSBio, Seattle, USA) levels were measured using an enzyme‐linked immunosorbent assay (ELISA).

Techniques: Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: HC diet inhibited testosterone synthesis by activating endoplasmic reticulum stress in testicular Leydig cells

doi: 10.1111/jcmm.14143

Figure Lengend Snippet: The improvement of Leydig cell function when blocking the ER stress. HC diet‐fed rats were treated with 4‐PBA and PBS for 8 wk. The levels of serum testosterone (A) were assayed after 4 and 8 wk of treatment. At the same time, the serum total cholesterol levels at the 8th wk (B) were determined. Representative protein expression and protein density analysis of the ratios among BiP, StAR and β‐actin were shown in the treated groups at the 8th wk (C). Representative images of immunofluorescence staining performed in the testes for StAR are shown for all treated groups (D). Data were expressed as the mean ±SEM (n = 10 per group). # P < 0.05 vs the HC group. All results were from at least three independent experiments.

Article Snippet: Serum total testosterone, intratesticular testosterone (Uscn Life Science & Technology Co., Ltd., Wuhan, China) and serum LH (LSBio, Seattle, USA) levels were measured using an enzyme‐linked immunosorbent assay (ELISA).

Techniques: Cell Function Assay, Blocking Assay, Expressing, Immunofluorescence, Staining

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice

doi: 10.1186/s12958-022-01032-y

Figure Lengend Snippet: Effect of sericin on reproductive hormones of photoperiod-induced mice: ( I ) testosterone and ( II ) aromatase. ( III - IV ) Principal component analysis (PCA), illustrating relationships between sperm quality/quantity and tissue testosterone and aromatase levels. ( V ) Pearson correlation coefficients for sperm parameters and reproductive hormones. Abbreviations: as in Fig.

Article Snippet: Testosterone (serum and testicular tissue) (Catalog number ER1462, FineTest, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China), aldosterone (Catalog number KGE016, R&D Systems, Inc., USA), corticosterone (Catalog number ARG80652, Arigo Biolaboratories Corp., Taiwan), melatonin (Catalog number LS-F25805, LifeSpan BioSciences, Inc., USA), Dehydroepiandrosterone (DHEA) (Catalog number EU2945, FineTest, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China), and testicular aromatase (Catalog number RK03515, ABclonal, Woburn, MA, USA) levels were measured by specific immunoenzymatic assays.

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice

doi: 10.1186/s12958-022-01032-y

Figure Lengend Snippet: Effect of sericin treatment of short and long photoperiod on the serum level of ( I ) testosterone, ( II ) dehydroepiandrosterone DHEA, ( III ) Corticosterone, ( IV ) Aldosterone, ( V ) Cholesterol, and ( VI ) Melatonin. Abbreviations: as in Fig. . Concerning Melatonin (VI) - the same letters denote homogenous groups when comparing experimental groups in a given week. A horizontal line above the bars means a homogenous group when comparing melatonin concentration obtained in subsequent weeks in a given experimental group (ANOVA, LSD test, p < 0.05)

Article Snippet: Testosterone (serum and testicular tissue) (Catalog number ER1462, FineTest, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China), aldosterone (Catalog number KGE016, R&D Systems, Inc., USA), corticosterone (Catalog number ARG80652, Arigo Biolaboratories Corp., Taiwan), melatonin (Catalog number LS-F25805, LifeSpan BioSciences, Inc., USA), Dehydroepiandrosterone (DHEA) (Catalog number EU2945, FineTest, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China), and testicular aromatase (Catalog number RK03515, ABclonal, Woburn, MA, USA) levels were measured by specific immunoenzymatic assays.

Techniques: Concentration Assay