Journal: bioRxiv

Article Title: Leptin activation of dorsal raphe neurons inhibits feeding behavior

doi: 10.1101/2023.04.24.538086

Figure Lengend Snippet: Serotonergic neurons sensitive to leptin project from the DRN to the ARC. (A) Fluorescently labeled CTb-555 was injected into the ARC and left for 1 month, then leptin was injected 1 hour before sacrifice to induce phosphorylation of STAT3 in the DRN. Confocal image taken with a 20× objective of the DRN, with leptin sensitive neurons (pSTAT3, green), neurons that project to the ARC (fCTb, red), and serotonergic neurons (TPH, blue). White arrow notes triple labeled neurons. (B) Image of the ARC taken with a 10× objective, where there are projections from leptin expressing neurons in the DRN (mCherry, red), and serotonergic reuptake transporter (SERT, green). (C) Image of ARC taken with a 40× objective showing colocalization of projections from leptin receptor expressing DRN neurons and SERT. Scalebars = 50µm across all images.

Article Snippet: Sections were then incubated with primary antibody (anti-Phosphorylated STAT3, Cell Signaling, Danvers, MA, #9145, 1:200; anti-Tryptophan Hydroxylase (TPH), Millipore Darmstadt, Germany, AB1541, 1:750; anti-SERT, Neuromics, Edina, MN RA24330, 1:500; anti-5-HT, Neuromics, GT20079, 1:1,000) and incubated overnight at 4°C.

Techniques: Labeling, Injection, Expressing