Journal: Journal of Microbiology and Biotechnology

Article Title: FTO-Mediated m6A Demethylation of SERPINF1 Attenuates Multiple Myeloma Progression via the Wnt/β-Catenin Pathway

doi: 10.4014/jmb.2510.10039

Figure Lengend Snippet: ( A ) Correlation between SERPINF1 expression and overall survival in MM analyzed using the public Kaplan–Meier plotter database. ( B ) SERPINF1 mRNA expression in clinical peripheral blood samples from 18 MM patients and 18 healthy donors analyzed via real-time quantitative polymerase chain reaction (RT-qPCR). Student’s t -test. ( C ) SERPINF1 mRNA expression measured in MM cell lines (U266B1, AMO1, and LP-1) and normal HNPC cells via RT-qPCR. Analysis of variance followed by Dunnett’s post hoc test. All data are presented as mean ± standard deviation from three independent experiments, each performed with three biological replicates ( n = 9).

Article Snippet: To investigate the role of SERPINF1 in vivo , nude mice (BALB/c; age, 4–6 weeks, male) purchased from Cyagen (China) were subcutaneously injected with U266B1 cells stably overexpressing SERPINF1 or the control vector.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Standard Deviation

Journal: Journal of Microbiology and Biotechnology

Article Title: FTO-Mediated m6A Demethylation of SERPINF1 Attenuates Multiple Myeloma Progression via the Wnt/β-Catenin Pathway

doi: 10.4014/jmb.2510.10039

Figure Lengend Snippet: ( A ) Representative tumor images from different groups 30 days after injecting U266B1 cells with stable SERPINF1 overexpression or control. ( B ) Graph illustrating the growth of xenograft tumor volumes over time in the SERPINF1-overexpressing and control groups. ( C ) Tumor weights measured 30 days after injecting U266B1 cells with stable SERPINF1 overexpression or control. ( D ) β-Catenin, c-Myc, and cyclin D1 protein expression levels analyzed and quantified using Western blotting. Student’s t -test. Data are presented as mean ± standard deviation (SD) from three independent experiments, each performed with three biological replicates ( n = 9).

Article Snippet: To investigate the role of SERPINF1 in vivo , nude mice (BALB/c; age, 4–6 weeks, male) purchased from Cyagen (China) were subcutaneously injected with U266B1 cells stably overexpressing SERPINF1 or the control vector.

Techniques: Over Expression, Control, Expressing, Western Blot, Standard Deviation

Journal: The ocular surface

Article Title: Pigment Epithelium-derived Factor Secreted by Corneal Epithelial Cells Regulates Dendritic Cell Maturation in Dry Eye Disease

doi: 10.1016/j.jtos.2020.05.002

Figure Lengend Snippet: Corneas were harvested on days 7 and 14 after DED induction. Age and sex-matched normal mice, placed in the standard vivarium, served as the controls. (A) Serpinf1 mRNA expression by the corneal epithelial cells was quantified using real-time PCR. (B) Protein expression of PEDF in the lysate of corneal epithelial cells was assessed in DED mice on days 7 and 14 after exposure to desiccating stress using ELISA. Data from the experiments are presented as mean ± SEM of three independent experiments, each consisting of four mice per group. (*p<0.05, **p<0.01, ***p<0.001,)

Article Snippet: The first-strand of cDNA was synthesized by random hexamers using reverse transcriptase (SuperScript III; Invitrogen Inc., Carlsbad, CA), and subsequently predesigned primers (Taqman PCR Mastermix and FAM dye-labeled primers; Applied Biosystems Inc, Foster City, CA) were used to perform RT-PCR for Serpinf1 (Mm00441267_g1), IL-6 (Mm00446190_m1), IL-1β (Mm00434228_m1), IL-17A (Mm00439619_m1), IL-23 (Mm00518984_m1).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Cancers

Article Title: Decreased Gene Expression of Antiangiogenic Factors in Endometrial Cancer: qPCR Analysis and Machine Learning Modelling

doi: 10.3390/cancers15143661

Figure Lengend Snippet: Details for the TaqMan “Assays on Demand” used for the 15 investigated genes and 2 reference genes.

Article Snippet: SERPINF1 , serpin family F member 1 , Hs00171467_m1.

Techniques: Derivative Assay

Journal: Cancers

Article Title: Decreased Gene Expression of Antiangiogenic Factors in Endometrial Cancer: qPCR Analysis and Machine Learning Modelling

doi: 10.3390/cancers15143661

Figure Lengend Snippet: Comparison of fold regulation (FR) in gene expression between tumour and tumour-adjacent control tissue.

Article Snippet: SERPINF1 , serpin family F member 1 , Hs00171467_m1.

Techniques: Comparison, Gene Expression, Control

Journal: Cancers

Article Title: Decreased Gene Expression of Antiangiogenic Factors in Endometrial Cancer: qPCR Analysis and Machine Learning Modelling

doi: 10.3390/cancers15143661

Figure Lengend Snippet: Strong correlations between mRNA expressions within the tumour and tumour-adjacent tissue and between both tissues, considering EC patients stratified according to LVI status.

Article Snippet: SERPINF1 , serpin family F member 1 , Hs00171467_m1.

Techniques: