Journal: EMBO reports

Article Title: SorLA restricts TNFα release from microglia to shape a glioma-supportive brain microenvironment.

doi: 10.1038/s44319-024-00117-6

Figure Lengend Snippet: Figure 1. SORL1 expression in human GAMs is linked to their functional properties.

Article Snippet: Reagent/resource Reference or source Identifier or catalog number Experimental models Human brain tissue samples Department of Neuropathology of the Amsterdam UMC NA HEK293 ATCC CRL-1573 BV2 Przanowski et al, 2018 NA GL261 luc+ /tdT+ Ochocka et al, 2021 NA GL261 WT Ochocka et al, 2021 NA C57BL/6J (M. musculus) Nencki Institute of Experimental Biology, PAS NA SorLA−/− (SorLA-KO/SLKO) C57BL/6J mice Andersen et al, 2005 NA Recombinant DNA pEGFPC2-BIO Swiech et al, 2011 NA GFP-TNFα Manderson et al, 2007 Addgene #28089 SorLA-ΔVPS10P This study NA SorLA-ΔEGF/β-propeller This study NA SorLA-ΔFN3 This study NA SorLA-ΔCR Mehmedbasic et al, 2015 NA SorLA-WT This study NA SorLA mini-VPS10P This study NA SorLA mini-EGF/β-propeller This study NA SorLA mini-FN3 This study NA SorLA mini-CR This study NA Antibodies Anti-Caspase-3 Cell Signaling CS9662 Anti-CD8-alpha Abcam ab217344 Reagent/resource Reference or source Identifier or catalog number Anti-Galectin-3 Biolegend M3/38 Anti-GAPDH Millipore MAB374 Anti-GFP Santa Cruz Biotechnology SC8334 Anti-GM130 BD Biosciences BD610823 Anti-GPX4 Abcam ab125066 Anti-Iba1 WAKO 019-19741 Anti-Iba1 (for iMG staining) Abcam ab5076 Anti-Lamp1 Sigma-Aldrich MABC39 Anti-MPO R&D Systems AF3667 Anti-myc-tag Cell Signaling 2278S Anti-P2RY12 Genetex GTX54796 Anti-PARP Cell Signaling CS9542 Anti-Rab7 Cell Signaling CS95746 Anti-p-RIP1 Cell Signaling CS83613 Anti-RIP1 Cell Signaling CS3493 Anti-p-RIP3 Abcam ab222320 Anti-RIP3 Abcam ab62344 Anti-p-STAT3 Cell Signaling CS9145 Anti-STAT3 Cell Signaling CS9145 Anti-Rab11 BD Biosciences BD610657 Anti-SorLA C-term, produced in rabbit Schmidt et al, 2007 NA Anti-SorLA BD Transduction Laboratories 611861 Anti-SorLA EMD Millipore MABN1793 Anti-SorLA, produced in goat Schmidt et al, 2016 NA Anti-Tmem119 Synaptic Systems 400002 Anti-TNFα Cell Signaling CS11948S Anti-TRFR Abcam ab269513 Anti-Vti1b BD Biosciences BD611404 Oligonucleotides and other sequence-based reagents Sorl1 TaqMan probe ThermoFisher Scientific Hs00983770; Mm01169526 TNFα TaqMan probe ThermoFisher Scientific Mm00443258 © The Author(s) EMBO reports 13 D ow nloaded from https://w w w .em bopress.org on A pril 24, 2024 from IP 113.173.236.9.

Techniques: Expressing, Functional Assay