Journal: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences

Article Title: Purple pigments, catechinopyranocyanidins A and B, in the seed coats of red adzuki beans elute during processing to yield purple-colored an-paste

doi: 10.2183/pjab.101.037

Figure Lengend Snippet: HPLC chromatograms of extracts of dried adzuki bean (cv. Shumari) and lyophilized sarashi-an obtained by beans cv. Shumari. Black lines: absorbance at 280 nm; purple lines: absorbance at 570 nm. * indicates purple pigment of different structures from 1 and 2 . (A) Dried adzuki bean extract. (B) Lyophilized sarashi-an with general processing. (C) Lyophilized sarashi-an with pre-vacuum processing.

Article Snippet: Analytical HPLC was performed on a JASCO HPLC system (Jasco, Hachioji, Japan) comprising two pumps, along with a mixer, a degasser, a PDA detector, a column oven, and an autosampler, all controlled using ChromNAV 2.0 HPLC software (Jasco, Hachioji, Japan) on a personal computer, as reported previously. )

Techniques:

Journal: Cell

Article Title: Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition

doi: 10.1016/j.cell.2020.06.026

Figure Lengend Snippet:

Article Snippet: Anti-Helios-PE-Vio615, human and mouse (REA829) , Miltenyi Biotec , Cat#130-112-636.

Techniques: Labeling, Plasmid Preparation, Purification, Recombinant, cDNA Synthesis, Suspension, Binding Assay, Control, Cell Isolation, Conjugation Assay, Sequencing, Transgenic Assay, Knock-Out, Software

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet: ( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as α7integrin+/Sca1− cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Produced, Reverse Transcription Polymerase Chain Reaction, Control, Isolation, Quantitative RT-PCR, Derivative Assay, Fractionation, Negative Control

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet:

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Control, Clone Assay, SYBR Green Assay, Magnetic Beads, Recombinant, Software