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segthresh  (MathWorks Inc)
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A) Z-projections of <t>SegThresh-segmented</t> Brainbow-labeled MG in IPL and OPL. Yellow, cell borders; gray, cell territories. B) The regions of A where two or more cells overlap are indicated in orange. C) Same fields of view as A, except that each cell has been flipped about its horizontal axis. D) Regions of overlap in flipped image. When cells are flipped, retinal coverage is decreased (more of the field of view is white in C than A), and overlap is increased (more orange in D than B), suggesting that the specific shapes of arbors relative to their neighbors is not random. E) Quantification of arbor overlap for interacting pairs of cells in the IPL and OPL. F,G) Pairwise comparison of arbor overlap for real and flipped neighboring cells (example pairs shown in F). Flipped territories demonstrate significantly more overlap than real territories (G). Colored squares, individual cell pairs; black circle, group mean. *IPL p=0.023; *OPL p=0.008 (see Results for statistical details). Scale bar: 10 µm (A–D).
Segthresh, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Z-projections of SegThresh-segmented Brainbow-labeled MG in IPL and OPL. Yellow, cell borders; gray, cell territories. B) The regions of A where two or more cells overlap are indicated in orange. C) Same fields of view as A, except that each cell has been flipped about its horizontal axis. D) Regions of overlap in flipped image. When cells are flipped, retinal coverage is decreased (more of the field of view is white in C than A), and overlap is increased (more orange in D than B), suggesting that the specific shapes of arbors relative to their neighbors is not random. E) Quantification of arbor overlap for interacting pairs of cells in the IPL and OPL. F,G) Pairwise comparison of arbor overlap for real and flipped neighboring cells (example pairs shown in F). Flipped territories demonstrate significantly more overlap than real territories (G). Colored squares, individual cell pairs; black circle, group mean. *IPL p=0.023; *OPL p=0.008 (see Results for statistical details). Scale bar: 10 µm (A–D).

Journal: The Journal of comparative neurology

Article Title: Anatomy and spatial organization of Müller glia in mouse retina

doi: 10.1002/cne.24153

Figure Lengend Snippet: A) Z-projections of SegThresh-segmented Brainbow-labeled MG in IPL and OPL. Yellow, cell borders; gray, cell territories. B) The regions of A where two or more cells overlap are indicated in orange. C) Same fields of view as A, except that each cell has been flipped about its horizontal axis. D) Regions of overlap in flipped image. When cells are flipped, retinal coverage is decreased (more of the field of view is white in C than A), and overlap is increased (more orange in D than B), suggesting that the specific shapes of arbors relative to their neighbors is not random. E) Quantification of arbor overlap for interacting pairs of cells in the IPL and OPL. F,G) Pairwise comparison of arbor overlap for real and flipped neighboring cells (example pairs shown in F). Flipped territories demonstrate significantly more overlap than real territories (G). Colored squares, individual cell pairs; black circle, group mean. *IPL p=0.023; *OPL p=0.008 (see Results for statistical details). Scale bar: 10 µm (A–D).

Article Snippet: The above algorithm was incorporated in an open-source software named “SegThresh” with an intuitive graphical user interface using MATLAB 2015a.

Techniques: Labeling, Comparison