Journal: International Journal of Molecular Sciences

Article Title: Serum and Tissue Light-Chains as Disease Biomarkers in AL Amyloidosis

doi: 10.3390/ijms26199511

Figure Lengend Snippet: Mechanisms of damage for free light chains. On the left, the direct cytotoxic effect of light chains on cells; on the right, the mass effect of amyloid deposition on tissue. In the myocardial cell on the left box, there is a brief representation of the molecular cascade of the direct damage .

Article Snippet: Sebia FLC ELISA kappa/lambda , Polyclonal , ELISA sandwich , AP22 Elite , - Larger measurement interval - Reduced repetitions needed for FLC quantification , - Proportional positive bias at high concentrations , - Quantitative with improved linearity across broad ranges.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Serum and Tissue Light-Chains as Disease Biomarkers in AL Amyloidosis

doi: 10.3390/ijms26199511

Figure Lengend Snippet: Normal range of kappa/lambda FLC ratio Freelite ® assay. The serum quantification of free light chains (FLCs) and the kappa/lambda ratio vary according to renal function. ( a ) A normal kappa/lambda ratio in case of normal renal function (0.26–1.65) has a 100% negative predictive value for light-chain cardiac amyloidosis (AL-CA). If a kidney disease is present, the normal range is 0.37–3.10. ( b ) Comparison of two widely used serum free light chain (sFLC) assays. Freelite ® (The Binding Site, Birmingham, UK) uses polyclonal antibodies and is available on multiple clinical chemistry analyzers. N Latex FLC (Siemens Healthineers, Erlangen, Germany) employs a monoclonal antibody mixture and is available exclusively on Siemens nephelometric platforms.

Article Snippet: Sebia FLC ELISA kappa/lambda , Polyclonal , ELISA sandwich , AP22 Elite , - Larger measurement interval - Reduced repetitions needed for FLC quantification , - Proportional positive bias at high concentrations , - Quantitative with improved linearity across broad ranges.

Techniques: Comparison, Binding Assay

Journal: European Journal of Medical Research

Article Title: Performance and comparison of FLCCheck: a novel serum free light chain assay for the Auxiliary Diagnosis of Multiple Myeloma

doi: 10.1186/s40001-025-02984-8

Figure Lengend Snippet: Comparison of N Latex FLC with Freelite in the determination of FLC. Passing-Bablok ( A ) and Bland–Altman ( B ) analyses were conducted using 115 serum samples obtained from patients diagnosed with newly identified or relapsed multiple myeloma, Waldenström’s macroglobulinemia, plasma cell leukemia, elevated immunoglobulin levels, leukemia, and lymphoplasmacytic lymphoma. The results for the determination of κ and λ FLC and the κ/λ ratio, as measured by N Latex FLC and Freelite assays, are presented. Bland–Altman plots demonstrate a concordance between N Latex FLC and Freelite measurements. A positive bias indicates higher values for the determination of FLC by Freelite compared with N Latex FLC

Article Snippet: Recent studies comparing Freelite with N Latex FLC or Sebia FLC indicate potential new iFLC/uFLC thresholds (≥ 100) for these assays [ , ].

Techniques: Comparison, Clinical Proteomics

Journal: European Journal of Medical Research

Article Title: Performance and comparison of FLCCheck: a novel serum free light chain assay for the Auxiliary Diagnosis of Multiple Myeloma

doi: 10.1186/s40001-025-02984-8

Figure Lengend Snippet: Comparison of Freelite with FLCCheck in the determination of FLC. The results of the Passing–Bablok analysis ( A ) and the Bland–Altman analysis ( B ) are presented. Bland–Altman plots demonstrate a concordance between Freelite and FLCCheck measurements. A positive bias indicates higher values for the determination of FLC by FLCCheck compared with Freelite

Article Snippet: Recent studies comparing Freelite with N Latex FLC or Sebia FLC indicate potential new iFLC/uFLC thresholds (≥ 100) for these assays [ , ].

Techniques: Comparison

Journal: European Journal of Medical Research

Article Title: Performance and comparison of FLCCheck: a novel serum free light chain assay for the Auxiliary Diagnosis of Multiple Myeloma

doi: 10.1186/s40001-025-02984-8

Figure Lengend Snippet: Discrepancies of κ/λ ratio values in the clinical course of treatment. A and B refer to Patient 1 and Patient 2. The κ/λ ratios were measured using Freelite, N Latex FLC, and FLCCheck during the clinical course of patients at the indicated time points (tp)

Article Snippet: Recent studies comparing Freelite with N Latex FLC or Sebia FLC indicate potential new iFLC/uFLC thresholds (≥ 100) for these assays [ , ].

Techniques: