Journal: EMBO Reports

Article Title: Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation

doi: 10.1038/s44319-025-00599-y

Figure Lengend Snippet: ( A ) Schematic diagram depicting the Tudor affinity resin. SMN Tudor domain shown in surface representation (light gray) is immobilized onto the Halo-link agarose resin via an N-terminal Halo-tag. The box on the right illustrates the recognition of sDMA by the aromatic cage of SMN Tudor domain. ( B ) Workflow for the molecular affinity strategy for arginine methylation profiling. ( C ) The enrichment efficiencies of molecular affinity strategy in three biological replicate experiments. ( D ) The number of methylation peptides and different methylarginine sites (monomethyl or dimethyl) identified from three biological replicates. ( E ) The reproducibility of molecular affinity strategy by comparing identified methylarginine sites in three biological replicates.

Article Snippet: Symmetric dimethylarginine (SDMA) , MedChemExpress , #HY-101410.

Techniques: Methylation

Journal: EMBO Reports

Article Title: Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation

doi: 10.1038/s44319-025-00599-y

Figure Lengend Snippet: The lines represent data fitting curves using 1:1 binding model at a fixed stoichiometry (N) of 1. The peptides used were SmD1 (AGRGRGR), SmD1-sDMA (AGR me2s GRGR), SmD1 aDMA (AGR me2a GRGR).

Article Snippet: Symmetric dimethylarginine (SDMA) , MedChemExpress , #HY-101410.

Techniques: Binding Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: Flow diagram of the literature search for studies on biomarkers on equine kidney disease. SDMA—symmetric dimethylarginine; NGAL—neutrophil gelatinase-associated lipocalin. Searches were always in combination with the search word horse or equine.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: List of studies on symmetric dimethylarginine (SDMA) concentrations in blood in horses and its analytical and clinical characteristics.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques: Comparison, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, High Throughput Screening Assay, Concentration Assay, Targeted Proteomics, Mass Spectrometry, Chromatography, Spectroscopy

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: Overview of molecular and physiological information on the identified kidney biomarkers.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques: Biomarker Discovery, Functional Assay, Methylation, Generated, Analogues, Transduction, Concentration Assay, Produced, Protease Inhibitor, Modification, Bacteria, Infection, Activation Assay, Filtration, Clinical Proteomics

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: (A) The IDEXX SDMA Test. (B) The DLD SDMA ELISA method. Solid line represents best fit linear model; shading represents 95% confidence interval around the line. Dashed line is the line of equality. Outliers are represented by *.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: Slopes and intercepts with 95% confidence intervals for the best fit linear models determined for all samples and for canine and feline samples individually.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: (A) The IDEXX SDMA Test. (B) The DLD SDMA ELISA method. Solid line represents best fit linear model; shading represents 95% confidence interval around the line. Dashed line is the line of equality. Outliers are represented by *.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: Systematic bias calculated from fit lines of IDEXX SDMA Test and DLD ELISA SDMA compared to LC-MS over a clinically relevant range of SDMA concentrations.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: (A) The IDEXX SDMA Test–Feline sample, 31.6 μg/dL (LC-MS). (B) The DLD SDMA ELISA method–Feline sample, 31.6 μg/dL (LC-MS). (C) The IDEXX SDMA Test–Canine sample, 11.8 μg/dL (LC-MS). (D) The DLD SDMA ELISA method–Canine sample, 11.8 μg/dL (LC-MS). Outliers are represented by *.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Comparative performance of IDEXX SDMA Test and the DLD SDMA ELISA for the measurement of SDMA in canine and feline serum

doi: 10.1371/journal.pone.0205030

Figure Lengend Snippet: Variation by assay for each pooled serum sample.

Article Snippet: IDEXX SDMA Test High , Feline , 34.7 , 2.3% , 0.8 (0.5, 1.5) , 0.8 (0.4, 1.8) , 0.1 (0, 0.5).

Techniques: Standard Deviation, Enzyme-linked Immunosorbent Assay