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Image Search Results
Journal: bioRxiv
Article Title: Iron addicted colorectal cancers exploit Heme-Complex II axis to resist oxidative cell death
doi: 10.1101/2025.11.05.686813
Figure Lengend Snippet: A) Schematic showing the xenograft tumor model with subcutaneous implantation. B) Tumor weight from B) C) Lipid ROS are measured by C11-BODIPY581/591 in EPCAM positive cells from the tumors from B). D) Schematic showing breeding strategy to generate tamoxifen inducible intestine specific SDHC deficient mice. E) Western blot images showing cellular and mitochondrial protein expression of SDHC 5days post tamoxifen induction via 100mg/kg body weight I.P. injection in WT ( Sdhc +/+), Het ( Sdhc +/-) and KO ( Sdhc -/-) mice. F) Representative H&E images of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. G) Inflammatory histology scored for small intestine and colon of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. H) Representative immunohistochemistry images showing accumulation of 4 hydroxynonenal (4HNE) in small intestine and colon of Sdhc F/F and Sdhc ΔIE mice fed with 350 ppm or 1000ppm iron containing chow for 7 days. I) Schematic showing treatment protocol for colitis associated cancer model using AOM/DSS in Sdhc F/F (WT) and Sdhc Het IE (Het) mice. J) Tumor number measured in mice from I). K) Tumor burden measured in mice from I) L) Lipid ROS measured by C11-BODIPY581/591 in EPCAM positive epithelial cells derived from I). M) Schematic showing role of heme-SDHC-CoQ axis in mitigating iron induced cell death in CRC cells. All data are mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons test for (B) and (G) and t test for (C), (J), (K) and (L). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Article Snippet: Followed by blocking with 5% goat serum in PBS and sections were then probed with primary
Techniques: Western Blot, Expressing, Injection, Immunohistochemistry, Derivative Assay