Journal: Scientific Reports

Article Title: PTH/SDF-1α cotherapy induces CD90+CD34− stromal cells migration and promotes tissue regeneration in a rat periodontal defect model

doi: 10.1038/srep30403

Figure Lengend Snippet: ( A ) H&E staining of the periodontal defects at 1, 2, 4 and 8 weeks post-surgery. The visual fields framed by the black line were magnified in the images below. The black arrows represented the border of the defect. B indicated new bone. D displayed the dentine of the native root. F represented newly formed fibers. ( B ) Quantitative analysis of newly formed bone areas in the four groups at four time points. * P < 0.05 compared with control group; ▲ P < 0.05 PTH group compared with SDF-1α group; § P < 0.05 PTH + SDF-1α group compared with other experimental groups.

Article Snippet: Left-side defects were implanted with collagen membranes loaded with SDF-1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA), while the right-side defects were implanted with collagen membranes loaded with PBS.

Techniques: Staining

Journal: Scientific Reports

Article Title: PTH/SDF-1α cotherapy induces CD90+CD34− stromal cells migration and promotes tissue regeneration in a rat periodontal defect model

doi: 10.1038/srep30403

Figure Lengend Snippet: ( A ) Immunofluorescence staining of CXCR4+ cells in four groups at four time points. ( B ) Quantitative analysis of the number of CXCR4+ cells showed that PTH + SDF-1α promoted the recruitment of CXCR4+ cells than that in the other three groups at the early stage of healing process (day 3, week 1 and week 2). The number of CXCR4+ cells in SDF-1α group was larger than that in PTH group at day 3 and week 1, and there was no difference at week 2. At week 4, CXCR4+ cells were hardly detected. * P < 0.05 compared with control group; ▲ P < 0.05 PTH group compared with SDF-1α group; § P < 0.05 PTH + SDF-1α group compared with other experimental groups.

Article Snippet: Left-side defects were implanted with collagen membranes loaded with SDF-1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA), while the right-side defects were implanted with collagen membranes loaded with PBS.

Techniques: Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: PTH/SDF-1α cotherapy induces CD90+CD34− stromal cells migration and promotes tissue regeneration in a rat periodontal defect model

doi: 10.1038/srep30403

Figure Lengend Snippet: ( A ) Immunofluorescence double staining of the four groups at four time points. ( B ) Quantitative analysis of the number of CD90+CD34− stromal cells showed that SDF-1α combined with PTH increased the recruitment of CD90+CD34− stromal cells than the other three groups at day 3, week 1 and 2. The number of CD90+CD34− stromal cells peaked at week 1 and reduced at week 2. At week 4, MSCs were hardly detected. White arrow indicated CD90+CD34− stromal cells. * P < 0.05 compared with control group; ▲ P < 0.05 PTH group compared with SDF-1α group; § P < 0.05 PTH + SDF-1α group compared with other experimental groups.

Article Snippet: Left-side defects were implanted with collagen membranes loaded with SDF-1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA), while the right-side defects were implanted with collagen membranes loaded with PBS.

Techniques: Immunofluorescence, Double Staining

Journal: Scientific Reports

Article Title: PTH/SDF-1α cotherapy induces CD90+CD34− stromal cells migration and promotes tissue regeneration in a rat periodontal defect model

doi: 10.1038/srep30403

Figure Lengend Snippet: ( A ) TRAP (red) staining at day 3, week 1, 2 and 4. ( B ) TRAP+ cells in four groups. * P < 0.05 compared with control group; ▲ P < 0.05 PTH group compared with SDF-1α group; § P < 0.05 PTH + SDF-1α compared with other experimental groups.

Article Snippet: Left-side defects were implanted with collagen membranes loaded with SDF-1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA), while the right-side defects were implanted with collagen membranes loaded with PBS.

Techniques: Staining

Journal: Scientific Reports

Article Title: PTH/SDF-1α cotherapy induces CD90+CD34− stromal cells migration and promotes tissue regeneration in a rat periodontal defect model

doi: 10.1038/srep30403

Figure Lengend Snippet: ( A,B ) Immunohistochemical staining of Runx2 (brown) and ALP (brown) at week 1, 2 and 4. ( C ) Immunohistochemical staining of Col I (brown) at week 2, 4 and 8. ( D–F ) Quantitative analyses of Runx2+ cells, ALP expression and Col I expression in four groups. * P < 0.05 compared with control group; ▲ P < 0.05 PTH group compared with SDF-1α group; § P < 0.05 PTH + SDF-1α compared with other experimental groups.

Article Snippet: Left-side defects were implanted with collagen membranes loaded with SDF-1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA), while the right-side defects were implanted with collagen membranes loaded with PBS.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Breast Cancer Research : BCR

Article Title: Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis

doi: 10.1186/bcr3053

Figure Lengend Snippet: Genes differentially regulated by IRF5 in MDA-MB-231 cells.

Article Snippet: Briefly, 100 ng/ml human recombinant CXCL12/SDF-1 (R&D Systems, Minneapolis, MN, USA) was added to 600 μl of phenol red-free DMEM medium supplemented with 10% FBS in the lower chamber.

Techniques: Expressing

Journal: Breast Cancer Research : BCR

Article Title: Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis

doi: 10.1186/bcr3053

Figure Lengend Snippet: IRF5 reduces CXCR4 cell surface expression and SDF-1/CXCL12-dependent chemotaxis of MDA-MB-231 cells . A . CXCR4 expression (grey line) in unstimulated cells, shown superimposed on the isotype control (grey shaded area), and CXCR4 expression (black line) after stimulation, was measured by flow cytometry. MDA-MB-231 cells (pBabe and pBIRF5) were treated with the CXCR4 ligand SDF-1 for six hours and CXCR4 expression measured. IRF5 expressing cells show no significant expression of CXCR4. M1, Marker 1. Representative histogram plots from three independent experiments performed in duplicate are shown. B . Cells overexpressing IRF5 are incapable of SDF-1-induced migration when compared to empty vector (EV pBabe) control cells. Data are expressed as mean ± SD of three independent experiments performed in duplicate. Statistical significance was determined by comparing the difference in number of cells migrated between pBabe and pBIRF5 cells; * denotes P < 0.02, ** P < 0.005. C . CXCR4 promoter reporter activity was analyzed by Dual Luciferase assay. MDA-231-pBabe and MDA-231-pBIRF5 were transfected with pGL3 empty vector or pGL3 CXCR4 5'Δ3 promoter and mock-treated with PBS or 100 ng/ml CXCL12. Data are expressed as the mean relative stimulation ± SD from three independent experiments performed in triplicate. Statistical significance was determined by comparing the difference in promoter activity between pBabe and pBIRF5 expressing cells; * denotes P < 0.05.

Article Snippet: Briefly, 100 ng/ml human recombinant CXCL12/SDF-1 (R&D Systems, Minneapolis, MN, USA) was added to 600 μl of phenol red-free DMEM medium supplemented with 10% FBS in the lower chamber.

Techniques: Expressing, Chemotaxis Assay, Control, Flow Cytometry, Marker, Migration, Plasmid Preparation, Activity Assay, Luciferase, Transfection