Journal: Scientific Reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Secretion of growth factors by adipose-derived stem cells (ASCs) and the formation of networks by co-culture with endothelial cells (ECs) or lymphatic endothelial cells (LECs). ( a ) The levels of vascular endothelial growth factor-C (VEGF-C), VEGF-A, hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in the medium bathing cultured ASCs were measured using enzyme-linked immunosorbent assays. HGF and VEGF-A were both secreted by ASCs, whereas the levels of secreted VEGF-C and b-FGF were much lower. ( b ) Green fluorescent protein (GFP)-expressing ECs and ASCs co-cultured at a ratio of 1:4. Networks of vascular ECs were observed, and the edges of the vascular EC network were sharp (arrows). ( c ) GFP-LECs and ASCs co-cultured at a ratio of 1:4. The LECs formed networks, but in contrast to vascular ECs, rounded/bulging structures were observed at the edges of the LEC network (arrowheads). ( d ) Merge image of ( c ) and phase-contrast image. ASCs shows random pattern with no specific character which do not form own network or support LEC network directly.

Article Snippet: The concentrations of VEGF-C, VEGF-A, HGF, and bFGF were measured by enzyme-linked immunosorbent assay (ELISA) using a Human VEGF-C ELISA Kit (P49767, RayBiotech, USA), LBIS Human VEGF ELISA Kit (631-40831, Fujifilm Wako, Japan), AuthentiKine Human HGF ELISA Kit (KE00168, Proteintech, USA) and Human bFGF ELISA Kit (P09038, RayBiotech, USA) in accordance with the manufacturers’ instructions.

Techniques: Derivative Assay, Co-Culture Assay, Cell Culture, Expressing

Journal: Molecular Medicine Reports

Article Title: Oleic acid induces A7r5 cell proliferation and migration associated with increased expression of HGF and p-p38

doi: 10.3892/mmr.2021.12123

Figure Lengend Snippet: Effects of OA on the expression of HGF in A7r5 cells. (A) ELISA results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P<0.01 and ***P<0.001 vs. 0 µM OA group; ## P<0.01 and ### P<0.001 vs. 50 µmol/l OA group. OA, oleic acid; HGF, hepatocyte growth factor.

Article Snippet: Commercial HGF ELISA kit (cat. no. EK1301) was purchased from Boster Biological Technology.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction