scale bars across micrographs Search Results


fluorescence micrographs carl zeiss lsm 780  (Carl Zeiss)
90
Carl Zeiss fluorescence micrographs carl zeiss lsm 780
a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell <t>fluorescence</t> maximum intensity projection <t>micrographs</t> (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control
Fluorescence Micrographs Carl Zeiss Lsm 780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scale bars across micrographs  (Biologica Environmental Services)
86
Biologica Environmental Services scale bars across micrographs
a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell <t>fluorescence</t> maximum intensity projection <t>micrographs</t> (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control
Scale Bars Across Micrographs, supplied by Biologica Environmental Services, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micrograph scale bars  (Gatan Inc)
97
Gatan Inc micrograph scale bars
a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell <t>fluorescence</t> maximum intensity projection <t>micrographs</t> (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control
Micrograph Scale Bars, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micrograph  (Oxford Instruments)
99
Oxford Instruments micrograph
a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell <t>fluorescence</t> maximum intensity projection <t>micrographs</t> (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control
Micrograph, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 200 scale bar  (Carl Zeiss)
90
Carl Zeiss axiovert 200 scale bar
a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell <t>fluorescence</t> maximum intensity projection <t>micrographs</t> (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control
Axiovert 200 Scale Bar, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control

Journal: Cell Death Discovery

Article Title: The palladacycle complex AJ-5 induces apoptotic cell death while reducing autophagic flux in rhabdomyosarcoma cells

doi: 10.1038/s41420-019-0139-9

Figure Lengend Snippet: a Western blotting of p62/SQSTM1 protein levels in RH30 and RD cells treated with vehicle (V), 0.1 µM or IC 50 AJ-5 for 24 and 48 h. b Western blotting showing LC3I and LC3II protein levels in RH30 and RD cells treated with vehicle (V) or IC 50 AJ-5 for 24 h followed by 2 h of treatment with 200 nM bafilomycin A1. For western blots, p38 was used as a loading control and densitometry readings were obtained using ImageJ. Protein expression levels are represented as a ratio of protein of interest/p38 normalized to vehicle control sample. Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 µM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ). Autophagosome flux J was calcuclated. Data were analysed using GraphPad Prism 6.0 and a parametric unpaired t -test was performed * p < 0.05, ** p < 0.01, *** p < 0.001. # compared to untreated control, * compared to vehicle control

Article Snippet: Blots are representative of at least two independent repeats. c Representative single-cell fluorescence maximum intensity projection micrographs (×630; Carl Zeiss LSM 780; scale bar is 20 μM) and pool size quantification of autophagy pathway intermediates: autophagosomes (GFP-LC3, nA ) (indicated with white arrows in the merged image), autolysosomes (LysoTracker Red, nAL ) and lysosomes (merged, nL ).

Techniques: Western Blot, Control, Expressing, Fluorescence