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laser speckle assisted perfusion images  (Perimed Inc)
86
Perimed Inc laser speckle assisted perfusion images
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Laser Speckle Assisted Perfusion Images, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/imaging+laser+speckle/pmc11897775-141-56-55
Average 86 stars, based on 1 article reviews
laser speckle assisted perfusion images - by Bioz Stars, 2026-09
86/100 stars
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lsm510 laserscanning microscope  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH lsm510 laserscanning microscope
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Lsm510 Laserscanning Microscope, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/lsm510+laserscanning+microscope/pmc05814268__oncotarget___09___7902___s001-29-6-7
Average 90 stars, based on 1 article reviews
lsm510 laserscanning microscope - by Bioz Stars, 2026-09
90/100 stars
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inkjet printed water based ag ink electrodes  (Verlag GmbH)
90
Verlag GmbH inkjet printed water based ag ink electrodes
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Inkjet Printed Water Based Ag Ink Electrodes, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/inkjet+printed+water+based+ag+ink+electrodes/10__1016_slash_j__mattod__2019__08__005-173-74-103
Average 90 stars, based on 1 article reviews
inkjet printed water based ag ink electrodes - by Bioz Stars, 2026-09
90/100 stars
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minimal synthetic media  (Difco)
86
Difco minimal synthetic media
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Minimal Synthetic Media, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/cette+contenus+dans+davis+des+dextrose+exp%C3%A9rience+influence+le+le+milieu+minimal+min%C3%A9ral+min%C3%A9raux+o+pour+sels+suppl%C3%A9ment+w/pm28607491-266-0-3
Average 86 stars, based on 1 article reviews
minimal synthetic media - by Bioz Stars, 2026-09
86/100 stars
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tetrahedral patchy particles  (Verlag GmbH)
90
Verlag GmbH tetrahedral patchy particles
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Tetrahedral Patchy Particles, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/tetrahedral+patchy+particles/pm37522252-259-17-3
Average 90 stars, based on 1 article reviews
tetrahedral patchy particles - by Bioz Stars, 2026-09
90/100 stars
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1024 confocal microscope  (Bio-Rad)
93
Bio-Rad 1024 confocal microscope
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
1024 Confocal Microscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/Liquichek+Immunoassay+Plus+Control/pm17091480-127-7-6
Average 93 stars, based on 1 article reviews
1024 confocal microscope - by Bioz Stars, 2026-09
93/100 stars
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vgcf pr-19-ps  (Applied Sciences Inc)
90
Applied Sciences Inc vgcf pr-19-ps
Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed <t>laser</t> <t>speckle-assisted</t> perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).
Vgcf Pr 19 Ps, supplied by Applied Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scale+bar+5/vgcf/pm16245518-99-6-11
Average 90 stars, based on 1 article reviews
vgcf pr-19-ps - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed laser speckle-assisted perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).

Journal: Molecular Therapy

Article Title: Tissue nanotransfection-based endothelial PLCγ2-targeted epigenetic gene editing rescues perfusion and diabetic ischemic wound healing

doi: 10.1016/j.ymthe.2025.01.034

Figure Lengend Snippet: Endothelial-targeted demethylation of Plcγ2 improves VEGF therapy on diabetic wound-edge vascularization (A) Vector components used for targeted demethylation of Plcγ2 promoter in STZ-induced diabetic mice using CRISPR-dCas9 approach. Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed laser speckle-assisted perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units. TNT procedure was done with scramble-gRNA, VEGF only, demethylation cocktail only, or VEGF plus demethylation cocktail CDH5 promoter-driven (endothelial) Plcγ2 gRNA in presence of dCas9-TET1CD cocktail. Perfusion was calculated based on the ratio of the wound area vs. distant normal skin. n = 6–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 + VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 + VEGF vs. VEGF (two-way ANOVA, followed by Tukey’s HSD post hoc test). (F) Blood flow and pulse pressure at the wound edge were measured using the pulse wave Doppler feature of Vevo 2100. The pulse wave profiles at d10 are shown (left). The increases in blood flow and pulsation as indicated by increase in peaks heights are shown for the respective groups compared to the control. The percentage of VTI and the calculated pulse pressure are shown as bar graphs (right). n = 6, ∗ p < 0.05 (one-way ANOVA, followed by Tukey’s HSD post hoc test). VTI, velocity time integral; scr gRNA, scramble-gRNA; VEGF, VEGF ORFs; gRNA PLCγ2 , gRNAs for Plcγ2 promoter; STZ, streptozotocin. (G) Wound closure was monitored at different days after wounding in abovementioned murine bipedicle ischemic wounds (D) subjected to TNT by digital planimetry (scale bar, 5mm) (H). n = 5–11, ∗ p < 0.05 gRNA PLCγ2 vs. scramble-gRNA; § p < 0.05 gRNA PLCγ2 +VEGF vs. scramble-gRNA; # p < 0.05 gRNA PLCγ2 +VEGF vs. VEGF (two-way ANOVA, followed by Tukey HSD post hoc test).

Article Snippet: Endothelial cells targeting was achieved by CDH5 promoter-driven gRNAs. (B) Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial demethylation cocktail in bipedicle ischemic wound model of STZ-induced diabetic mice. (C) Blood glucose level in STZ-induced diabetic mice used in this study. n = 17, ∗ p < 0.05 (Student’s t test). (D) Respective PeriMed laser speckle-assisted perfusion images (scale bar, 5 mm) and (E) their analysis data presented as arbitrary perfusion units.

Techniques: Plasmid Preparation, CRISPR, Control