anti murine brca1 antibody (Santa Cruz Biotechnology)

Santa Cruz Biotechnology anti murine brca1 antibody

Figure 1. <t>Brca1+/+</t> and Brca1-/- MMECs. (A) Schema for the generation of Brca1+/+ and Brca1-/- MMECs. Mammary glands were isolated from transgenic Brca1fl/fl mice and digested with collagenase and hyalouronidase to harvest cells. These cells were infected in vitro with HPV-16E6 retrovirus to generate Brca1+/+ MMECs. Brca1-/- MMECs were generated from these cells by transduction with the pBabe-Cre retrovirus to delete exon 11 sequences from both alleles of Brca1 gene. (B) Schema for the detection of Cre-mediated recombination for the deletion of the Brca1 exon 11. (C) PCR amplification using primers (1130, 1132) that flank the loxP sites around exon 11 of Brca1 gene resulted in a 600 bp product in Brca1-/- MMECs but not in Brca1+/+ MMECs. Deletions at the loxP sites generated a specific 600 bp PCR product. (D) PCR analysis using primers within exon 11 of Brca1 detects a 592 bp fragment in Brca1+/+ MMECs but not in the Brca1-/-

Anti Murine Brca1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti murine brca1 antibody - by Bioz Stars, 2026-09

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Figure 1. Brca1+/+ and Brca1-/- MMECs. (A) Schema for the generation of Brca1+/+ and Brca1-/- MMECs. Mammary glands were isolated from transgenic Brca1fl/fl mice and digested with collagenase and hyalouronidase to harvest cells. These cells were infected in vitro with HPV-16E6 retrovirus to generate Brca1+/+ MMECs. Brca1-/- MMECs were generated from these cells by transduction with the pBabe-Cre retrovirus to delete exon 11 sequences from both alleles of Brca1 gene. (B) Schema for the detection of Cre-mediated recombination for the deletion of the Brca1 exon 11. (C) PCR amplification using primers (1130, 1132) that flank the loxP sites around exon 11 of Brca1 gene resulted in a 600 bp product in Brca1-/- MMECs but not in Brca1+/+ MMECs. Deletions at the loxP sites generated a specific 600 bp PCR product. (D) PCR analysis using primers within exon 11 of Brca1 detects a 592 bp fragment in Brca1+/+ MMECs but not in the Brca1-/-

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMS.

doi: 10.4161/cc.3.11.1211

Figure Lengend Snippet: Figure 1. Brca1+/+ and Brca1-/- MMECs. (A) Schema for the generation of Brca1+/+ and Brca1-/- MMECs. Mammary glands were isolated from transgenic Brca1fl/fl mice and digested with collagenase and hyalouronidase to harvest cells. These cells were infected in vitro with HPV-16E6 retrovirus to generate Brca1+/+ MMECs. Brca1-/- MMECs were generated from these cells by transduction with the pBabe-Cre retrovirus to delete exon 11 sequences from both alleles of Brca1 gene. (B) Schema for the detection of Cre-mediated recombination for the deletion of the Brca1 exon 11. (C) PCR amplification using primers (1130, 1132) that flank the loxP sites around exon 11 of Brca1 gene resulted in a 600 bp product in Brca1-/- MMECs but not in Brca1+/+ MMECs. Deletions at the loxP sites generated a specific 600 bp PCR product. (D) PCR analysis using primers within exon 11 of Brca1 detects a 592 bp fragment in Brca1+/+ MMECs but not in the Brca1-/-

Article Snippet: Brca1 and p53 proteins were examined in cell lysates by western blot analyses with an anti-murine Brca1 antibody (mAb1)32 and a rabbit polyclonal anti-p53 antibody (FL-393) (Santa Cruz).

Techniques: Isolation, Transgenic Assay, Infection, In Vitro, Generated, Transduction, Amplification

Figure 2 (Above). Growth Kinetics of Brca1+/+ and Brca-/- MMECs. Brca1+/+

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMS.

doi: 10.4161/cc.3.11.1211

Figure Lengend Snippet: Figure 2 (Above). Growth Kinetics of Brca1+/+ and Brca-/- MMECs. Brca1+/+

Techniques:

$Figure 3 (Above). Sensitivity of Brca-/- MMECs to CDDP. Brca1+/+ and Brca1-/- MMECs were exposed to increasing concentrations of CDDP as described in materials and methods. After incubation at 37˚C for 7 days, MTT assay was performed. Cell survival was expressed as the fraction of CDDP-treated surviving cells to the untreated cells. The results are shown as the mean ± S.D. of seven independent experiments each done in quadru- plicate samples.$

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMS.

doi: 10.4161/cc.3.11.1211

Figure Lengend Snippet: Figure 3 (Above). Sensitivity of Brca-/- MMECs to CDDP. Brca1+/+ and Brca1-/- MMECs were exposed to increasing concentrations of CDDP as described in materials and methods. After incubation at 37˚C for 7 days, MTT assay was performed. Cell survival was expressed as the fraction of CDDP-treated surviving cells to the untreated cells. The results are shown as the mean ± S.D. of seven independent experiments each done in quadru- plicate samples.

Techniques: Incubation, MTT Assay

Figure 4. CDDP treatment induces apoptosis in Brca1-deficient MMECs. (A) Brca1+/+ MMECs and (B) Brca1-/- MMECs were either untreated or treated with increasing doses of CDDP for 72 hours. Seventy two hours after CDDP treatment, FACS analysis was performed as described in Materials and Methods. (C) Brca1+/+ and Brca1-/- MMECs were either untreated or treated with 40 µM of CDDP for 48 hours. Cells were identified for apoptosis by DAPI staining and fluorescence microscopy as described in materials and methods. (D) Brca1+/+ and Brca1-/- MMECs were either untreated or treated with 40 µM of CDDP for 0–24 hours or, (E) 0–40 µM of CDDP for 12 hours. Cells were stained with DAPI and the percentage of DAPI positive cells is shown as the mean ± S.D. of quadruplicate samples.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMS.

doi: 10.4161/cc.3.11.1211

Figure Lengend Snippet: Figure 4. CDDP treatment induces apoptosis in Brca1-deficient MMECs. (A) Brca1+/+ MMECs and (B) Brca1-/- MMECs were either untreated or treated with increasing doses of CDDP for 72 hours. Seventy two hours after CDDP treatment, FACS analysis was performed as described in Materials and Methods. (C) Brca1+/+ and Brca1-/- MMECs were either untreated or treated with 40 µM of CDDP for 48 hours. Cells were identified for apoptosis by DAPI staining and fluorescence microscopy as described in materials and methods. (D) Brca1+/+ and Brca1-/- MMECs were either untreated or treated with 40 µM of CDDP for 0–24 hours or, (E) 0–40 µM of CDDP for 12 hours. Cells were stained with DAPI and the percentage of DAPI positive cells is shown as the mean ± S.D. of quadruplicate samples.

Techniques: Staining, Fluorescence, Microscopy

Figure 5. Sensitivity of Brca-/- MMECs to MMS. Brca1+/+ and Brca1-/-

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMS.

doi: 10.4161/cc.3.11.1211

Figure Lengend Snippet: Figure 5. Sensitivity of Brca-/- MMECs to MMS. Brca1+/+ and Brca1-/-

Techniques:

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