Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Induction of potent TRAIL-mediated tumoricidal activity by hFLEX/Furin/TRAIL recombinant DNA construct.

doi: 10.1016/j.ymthe.2004.02.021

Figure Lengend Snippet: FIG. 2. Western blot analysis. (A) Expression of pFETZ-, pFFT-, and pFFZT-encoded proteins in COS7 cells following transfection. The presence of fusion proteins and cleaved protein products was detected using anti-TRAIL Ab. (B) Expression of pFFT, pFFZT, and pFETZ in vivo. pFFT-, pFFZT-, and pFETZ-encoded proteins could be detected in sera collected from mice injected with pFFT, pFFZT, or pFETZ with (i) anti-TRAIL Ab and (ii) anti-Flt3L Ab. (C) pFFT-, pFFZT-, or pFETZ- encoded proteins were consistently detected in sera collected from groups of mice injected with pFFT, pFFZT, or pFETZ, respectively. (D) Analysis of FFT, FFZT, and FETZ protein by 6% native PAGE. Cleaved form of TRAIL was assembled in stable trimers upon addition of isoleucine zipper sequence (arrow) compared to the monomeric cleaved TRAIL (dotted-line arrow).

Article Snippet: The membrane was then incubated with 5 Ag/ml rabbit anti-TRAIL polyclonal antibody or goat anti-Flt3L polyclonal antibody (Santa Cruz Biotechnology) for 1.5 h at room temperature.

Techniques: Western Blot, Expressing, Transfection, In Vivo, Injection, Clear Native PAGE, Sequencing