Journal: Scientific Reports

Article Title: Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma

doi: 10.1038/s41598-017-09539-w

Figure Lengend Snippet: Cell function assays. Knockdown TMSB4X in SAS cells may inhibit their cell proliferation and invasion ability in vitro , as well as suppress cervical LN metastasis in vivo . ( A ) The panel of TMSB4X expression level in 6 HNSCC cell lines by RT-PCR. The relative aggressive SAS cells have abundant TMSB4X. ( B ) In TMSB4X knockdown clones of SAS, the expression was revealed by RT-PCR. ( C ) The growth curves of TMSB4X knockdown clones of SAS. ( D ) The invasion ability of TMSB4X knockdown clones of SAS was performed by Boyden chamber assay. Cell photography and number were taken and counted at 200x magnification (high power field, HPF). ( E ) Bioluminescence images were taken from the buccal orthotopic xenograft mouse model. The total flux of bioluminescence was measured separately at mouse buccal (Tumor) and neck lymph node (LN metastasis). ( F ) The box-plot of LN metastasis was drawn according to the total flux value in region of interest (ROI). Asteroid *denotes P < 0.05. Statistics was calculated by student t-test. (shTb4-1: clone1 with shRNA-TMSB4X).

Article Snippet: Knockdown TMSB4X in SAS cells may inhibit their cell proliferation and invasion ability in vitro , as well as suppress cervical LN metastasis in vivo . ( A ) The panel of TMSB4X expression level in 6 HNSCC cell lines by RT-PCR.

Techniques: Cell Function Assay, Knockdown, In Vitro, In Vivo, Expressing, Reverse Transcription Polymerase Chain Reaction, Clone Assay, Boyden Chamber Assay, shRNA