sapi Search Results


sapi  (New England Biolabs)
98
New England Biolabs sapi
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Sapi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/SapI/pmc13676805-59-13-14
Average 98 stars, based on 1 article reviews
sapi - by Bioz Stars, 2026-11
98/100 stars
  Buy from Supplier
pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w  (Addgene inc)
93
Addgene inc pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Pklv2 2 Mu6grna5 Sapi Hu6grna5 Bbsi Pgkpurobfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/pKLV2%2E2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W+(Plasmid+%2372667)/pmc11999688-231-10-11
Average 93 stars, based on 1 article reviews
pklv2 2 mu6grna5 sapi hu6grna5 bbsi pgkpurobfp w - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
pklv2 2 h7skgrna5 sapi hu6grna5 bbsi pgkpurobfp w  (Addgene inc)
93
Addgene inc pklv2 2 h7skgrna5 sapi hu6grna5 bbsi pgkpurobfp w
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Pklv2 2 H7skgrna5 Sapi Hu6grna5 Bbsi Pgkpurobfp W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/pKLV2%2E2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W+(Plasmid+%2372666)/pmc11020096-65-13-14
Average 93 stars, based on 1 article reviews
pklv2 2 h7skgrna5 sapi hu6grna5 bbsi pgkpurobfp w - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
pjep317 paav u6sacas9gnra sapi efs gfp kash pa  (Addgene inc)
93
Addgene inc pjep317 paav u6sacas9gnra sapi efs gfp kash pa
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Pjep317 Paav U6sacas9gnra Sapi Efs Gfp Kash Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP-+KASH-pA+(Plasmid+%23113694)/pmc11699774-73-0-7
Average 93 stars, based on 1 article reviews
pjep317 paav u6sacas9gnra sapi efs gfp kash pa - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
paav u6 sacas9grna sapi cmv sacas9 dio pa  (Addgene inc)
93
Addgene inc paav u6 sacas9grna sapi cmv sacas9 dio pa
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Paav U6 Sacas9grna Sapi Cmv Sacas9 Dio Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA+(Plasmid+%23113691)/pmc09107327-831-7-8
Average 93 stars, based on 1 article reviews
paav u6 sacas9grna sapi cmv sacas9 dio pa - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
sapi  (Ceradyne Inc)
90
Ceradyne Inc sapi
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Sapi, supplied by Ceradyne Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/sapi/us08176830-300-4-7
Average 90 stars, based on 1 article reviews
sapi - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
sapi/ecori fragment of genscript-fvii-037  (GenScript corporation)
90
GenScript corporation sapi/ecori fragment of genscript-fvii-037
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Sapi/Ecori Fragment Of Genscript Fvii 037, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/sapi+ecori+fragment+of+genscript+fvii+037/us10968442-1397-1-4
Average 90 stars, based on 1 article reviews
sapi/ecori fragment of genscript-fvii-037 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
plasmid ppoli-sapi-ribozyme  (Promega)
90
Promega plasmid ppoli-sapi-ribozyme
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Plasmid Ppoli Sapi Ribozyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/plasmid+ppoli+sapi+ribozyme/pmc01212590-45-17-29
Average 90 stars, based on 1 article reviews
plasmid ppoli-sapi-ribozyme - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
goldengate-mediated directional cloning with sapi  (GoldenGate Software Inc)
90
GoldenGate Software Inc goldengate-mediated directional cloning with sapi
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Goldengate Mediated Directional Cloning With Sapi, supplied by GoldenGate Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/goldengate+mediated+directional+cloning+with+sapi/pm37822043-307-12-11
Average 90 stars, based on 1 article reviews
goldengate-mediated directional cloning with sapi - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
peraturan menteri pertanian nomor :19/permentan / ot.140/2/2010 tentang pedoman umum program swasembada daging sapi 2014  (Nomor Research GmbH)
90
Nomor Research GmbH peraturan menteri pertanian nomor :19/permentan / ot.140/2/2010 tentang pedoman umum program swasembada daging sapi 2014
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Peraturan Menteri Pertanian Nomor :19/Permentan / Ot.140/2/2010 Tentang Pedoman Umum Program Swasembada Daging Sapi 2014, supplied by Nomor Research GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/peraturan+menteri+pertanian+nomor++19+permentan+++ot+140+2+2010+tentang+pedoman+umum+program+swasembada+daging+sapi+2014/10__5398_slash_tasj__2022__45__1__9-210-3-6
Average 90 stars, based on 1 article reviews
peraturan menteri pertanian nomor :19/permentan / ot.140/2/2010 tentang pedoman umum program swasembada daging sapi 2014 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
customized sapi-free puc plasmid  (GenScript corporation)
90
GenScript corporation customized sapi-free puc plasmid
Miniaturization of plasmid vectors. (a) Validation of 9 <t>kB</t> <t>dCas9-VP64-EGFP-2XEsp3I</t> plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( <t>SapI</t> + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.
Customized Sapi Free Puc Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sapi/customized+sapi+free+puc+plasmid/pmc07755240-216-9-18
Average 90 stars, based on 1 article reviews
customized sapi-free puc plasmid - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Miniaturization of plasmid vectors. (a) Validation of 9 kB dCas9-VP64-EGFP-2XEsp3I plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( SapI + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.

Journal: Molecular biology reports

Article Title: Miniaturization of CRISPRa plasmids for efficient delivery into renal epithelial cells and Pkd1 transactivation

doi: 10.1007/s11033-026-11872-1

Figure Lengend Snippet: Miniaturization of plasmid vectors. (a) Validation of 9 kB dCas9-VP64-EGFP-2XEsp3I plasmid miniaturization to 6 kB mdCas9-VP64-EGFP vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 displays the products of the simultaneous digestion-ligation reaction ( Esp3I + T4 Ligase). Band 1 represents the re-circularized region of interest (ROI) i.e mini plasmid and band 2 the re-circularized vector backbone. The higher molecular weight bands likely represent concatemers. Lane 2 shows Esp3I digestion alone, showing the position of bands for the linearized ROI (band 1) and linearized vector backbone (band 2). Lane 3 shows the undigested full-length plasmid control. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final products following Esp3I and T5 exonuclease treatment: the circular mdCas9-VP64-EGFP mini plasmid (band 1) and the circular vector backbone (band 2). Lanes 6 and 7 serve as controls, demonstrating T5 exonuclease activity on linearized and undigested templates, respectively. ( b) Plasmid map of circular 5.9 kB mdCas9-VP64-EGFP mini plasmid. (c) Validation of 11.3 kB dCas9-VPR-2XSapI plasmid miniaturization to 6.7 kB mdCas9-VPR vector by agarose gel electrophoresis. The bands of interest are indicated by numbers to the right of the lane. Lane 1 shows the products of the simultaneous digestion-ligation reaction ( SapI + T4 Ligase); band 1 represents higher-molecular weight concatemers, band 2 represents the re-circularized region of interest (ROI) i.e mdCas9-VPR, and bands 3, 4, and 5 represent linear vector backbone fragments. Lane 2 shows SapI -only digestion, generating the linear ROI and multiple backbone fragments. Lane 3 shows the undigested parent plasmid. Lane 4 contains the DNA molecular weight marker. Lane 5 shows the final reaction products post- SapI and T5 exonuclease treatment; Band 2 is the T5-resistant circular mdCas9-VPR plasmid. As the backbone fragments lacked complementary overhangs, they remained linear and were degraded by T5, while higher molecular weight circular concatemers remain visible (Lane 5, above band 1). Lanes 6 and 7 serve as controls for T5 exonuclease activity. (d) Plasmid map of circular 6.7 kB mdCas9-VPR vector.

Article Snippet: The resulting ~11.3 kB dCas9-VPR-2XSapI ( Supplementary Fig. 2 ) was miniaturized using SapI (NEB #R0569S) to produce mdCas9-VPR, with all other reagents, volumes and concentrations identical to those described above ( Supplementary Table 1 ).

Techniques: Plasmid Preparation, Biomarker Discovery, Agarose Gel Electrophoresis, Ligation, Molecular Weight, Control, Marker, Activity Assay