salt buffer Search Results


ficoll 400  (Biotium)
90
Biotium ficoll 400
Ficoll 400, supplied by Biotium, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/5-Aminoallyl-dUTP%2C+Sodium+Salt%2C+10+mM+in+TE+Buffer/10__1038_slash_nprot__2011__417-126-165-161
Average 90 stars, based on 1 article reviews
ficoll 400 - by Bioz Stars, 2026-09
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high salt chip buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology high salt chip buffer
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
High Salt Chip Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/ChIP+Lysis+Buffer+High+Salt/pmc03976374-53-11-56
Average 93 stars, based on 1 article reviews
high salt chip buffer - by Bioz Stars, 2026-09
93/100 stars
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sodium dodecyl sulfate  (Valiant Co Ltd)
96
Valiant Co Ltd sodium dodecyl sulfate
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Sodium Dodecyl Sulfate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/Sodium+dodecyl+sulfate/pm41875353-168-0-4
Average 96 stars, based on 1 article reviews
sodium dodecyl sulfate - by Bioz Stars, 2026-09
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buffer components  (Chem Impex International)
95
Chem Impex International buffer components
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Buffer Components, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/Isopropyl+alcohol/pm41753796-55-0-5
Average 95 stars, based on 1 article reviews
buffer components - by Bioz Stars, 2026-09
95/100 stars
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anhydrous sodium carbonate  (Chem Impex International)
95
Chem Impex International anhydrous sodium carbonate
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Anhydrous Sodium Carbonate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/Sodium+carbonate/10__3390_slash_pr10102093-43-0-19
Average 95 stars, based on 1 article reviews
anhydrous sodium carbonate - by Bioz Stars, 2026-09
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disodium hydrogen phosphate  (Chem Impex International)
95
Chem Impex International disodium hydrogen phosphate
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Disodium Hydrogen Phosphate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/Sodium+phosphate+dibasic+dihydrate/pm34467346-104-103-129
Average 95 stars, based on 1 article reviews
disodium hydrogen phosphate - by Bioz Stars, 2026-09
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tris  (Chem Impex International)
95
Chem Impex International tris
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Tris, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/Tris(hydroxymethyl)aminomethane/pm36643556-41-0-7
Average 95 stars, based on 1 article reviews
tris - by Bioz Stars, 2026-09
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sodium cacodyl buffer  (Carl Roth GmbH)
90
Carl Roth GmbH sodium cacodyl buffer
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Sodium Cacodyl Buffer, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/cacodylic+acid+sodium+salt+trihydrate+buffer/pmc05849139-110-8-15
Average 90 stars, based on 1 article reviews
sodium cacodyl buffer - by Bioz Stars, 2026-09
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calcium- and magnesium-free hanks’ balanced salt solution hbss  (EuroClone)
90
EuroClone calcium- and magnesium-free hanks’ balanced salt solution hbss
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Calcium And Magnesium Free Hanks’ Balanced Salt Solution Hbss, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/hanks%E2%80%99+buffered+salt+solution/pm32100016-61-6-14
Average 90 stars, based on 1 article reviews
calcium- and magnesium-free hanks’ balanced salt solution hbss - by Bioz Stars, 2026-09
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hank's balanced saline solution (hbss)  (Welgene inc)
90
Welgene inc hank's balanced saline solution (hbss)
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Hank's Balanced Saline Solution (Hbss), supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/hank%E2%80%99s+buffer+salt+solution/pmc02386914-40-17-22
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hank's balanced saline solution (hbss) - by Bioz Stars, 2026-09
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alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt  (Promega)
90
Promega alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt
(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), <t>ChIP</t> experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments <t>cross-linked</t> <t>chromatin</t> was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
Alkaline Phosphatase Substrate Buffer Containing Nitro Blue Tetrazolium Chloride/5 Bromo 4 Chloro 3’ Indolyphosphate P Toluidine Salt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/alkaline+phosphatase+substrate+buffer+containing+nitro+blue+tetrazolium+chloride+5+bromo+4+chloro+3%E2%80%99+indolyphosphate+p+toluidine+salt/pmc03709059-62-81-88
Average 90 stars, based on 1 article reviews
alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt - by Bioz Stars, 2026-09
90/100 stars
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sumo buffer (salt-free)  (MCLAB Inc)
90
MCLAB Inc sumo buffer (salt-free)

Sumo Buffer (Salt Free), supplied by MCLAB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/sumo+buffer++salt+free+/pmc09762709-323-17-12
Average 90 stars, based on 1 article reviews
sumo buffer (salt-free) - by Bioz Stars, 2026-09
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(A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), ChIP experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments cross-linked chromatin was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).

Journal: PLoS ONE

Article Title: Nucleosomes Are Stably Evicted from Enhancers but Not Promoters upon Induction of Certain Pro-Inflammatory Genes in Mouse Macrophages

doi: 10.1371/journal.pone.0093971

Figure Lengend Snippet: (A and B), Nucleosome occupancy at a putative enhancer 10 kb upstream of the TSS of IL1A was determined in BMDMs prior to (blue bars and lines) and upon 1.5 h (yellow) or 3 h (red) induction with 1 μg/ml LPS as described in the legend of . ConSite predicted consensus sites for PU.1, C/EBP, IRF, AP1 and NFκB are indicated. (C and D), Nucleosome occupancy at the promoter of IL1A was determined as described in panel (A) in a region surrounding the TSS of IL1A. The TSS (black bar) and a putative TATA-box (blue bar) is indicated in panel (C). (E), Kinetics of nucleosome removal at IL1A and IL12B. BMDMs were induced with LPS for 15′, 30′, 60′ and 90′, and nucleosome occupancy was analyzed as described in (A). Nucleosome removal at three locations in the distal enhancer of IL12B (red lines) and in the enhancer of IL1A (blue lines) is shown. The data is displayed as the fold change in nucleosome occupancy over the levels found before induction at each location. The absence of changes in nucleosome occupancy at the TSS of IL12B (black line) and of IL1A (gray line) is shown for comparison. (F), ChIP experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue), H3K4me1 (green), H3K4me3 (yellow) and H3K27ac (red) were performed as described in the . For these experiments cross-linked chromatin was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).

Article Snippet: For all other ChIP experiments isolated chromatin was directly diluted with High Salt ChIP buffer and incubated with either 1 μg of anti-PolI antibody (sc-56767), 6 μg anti-TBP (sc-204), 4 μg anti-PU.1 (sc-352), 4 μg anti-C/EBPβ (sc-150), 6 μg anti-NFκB (sc-372), 5 μg anti-c-Jun (sc-45), 6 μg anti-p300 (sc-585) or 10 μg anti-IRF3 (sc-9082) all from Santa Cruz Biotechnologies.

Techniques: Comparison, Incubation, Binding Assay, Control

(A and B), ChIP experiments were performed as described in the legend of with antibodies that detect (A) PolII or (B) TBP in BMDMs before (dark blue bars), and upon 1.5 h (yellow) or 3 h (red) LPS induction. Cross-linked chromatin was either untreated (solid bars), or lightly digested with MNase (hatched bars) as described in the . The data was normalized to a region in the KIT promoter and genomic locations are indicated. The experiment was performed twice and error bars indicating the SEM are shown.

Journal: PLoS ONE

Article Title: Nucleosomes Are Stably Evicted from Enhancers but Not Promoters upon Induction of Certain Pro-Inflammatory Genes in Mouse Macrophages

doi: 10.1371/journal.pone.0093971

Figure Lengend Snippet: (A and B), ChIP experiments were performed as described in the legend of with antibodies that detect (A) PolII or (B) TBP in BMDMs before (dark blue bars), and upon 1.5 h (yellow) or 3 h (red) LPS induction. Cross-linked chromatin was either untreated (solid bars), or lightly digested with MNase (hatched bars) as described in the . The data was normalized to a region in the KIT promoter and genomic locations are indicated. The experiment was performed twice and error bars indicating the SEM are shown.

Article Snippet: For all other ChIP experiments isolated chromatin was directly diluted with High Salt ChIP buffer and incubated with either 1 μg of anti-PolI antibody (sc-56767), 6 μg anti-TBP (sc-204), 4 μg anti-PU.1 (sc-352), 4 μg anti-C/EBPβ (sc-150), 6 μg anti-NFκB (sc-372), 5 μg anti-c-Jun (sc-45), 6 μg anti-p300 (sc-585) or 10 μg anti-IRF3 (sc-9082) all from Santa Cruz Biotechnologies.

Techniques:

Journal: eLife

Article Title: Critical roles for ‘housekeeping’ nucleases in type III CRISPR-Cas immunity

doi: 10.7554/eLife.81897

Figure Lengend Snippet:

Article Snippet: Fractions containing the desired protein were pooled and mixed with SUMO Protease (MCLAB, CA) with the provided SUMO buffer (salt-free).

Techniques: CRISPR, Isolation, Recombinant, Over Expression, Purification, Sequencing, Construct, Plasmid Preparation, Protein Purification, Software