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Image Search Results
Journal: Vaccine
Article Title: Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathology
doi: 10.1016/j.vaccine.2022.08.049
Figure Lengend Snippet: Induction of significant Th1 response by using ODN2006 as an adjuvant. ( A ) Spleen and cervical lymph nodes were collected from individuals shown in (A). Single cell suspensions obtained from each tissue was cultured under the stimulation of peptide pool of SARS-CoV-2 S protein. Spleen ( B - D ) or cervical lymph node cells ( E - G ) were counted on the production of IFN-r ( B , E ), IL-4 ( C , F ) and IL-5 ( D , G ) by ELISpot assay. Data shown as the means ± SD. ( H , I ) SARS-CoV-2 S-specific IgG1 and IgG2a antibodies were quantified by ELISA, and ( J ) IgG1/IgG2a ratio was calculated. Data shown as the geometric means ± geometric SD. The p-values were calculated by Kruskal-Wallis test followed by Dunn’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001).
Article Snippet: Spots formed by cytokine-secreting cells were counted and analyzed using
Techniques: Adjuvant, Cell Culture, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay, Comparison