Journal: Journal of Clinical Pathology

Article Title: Unusual fusion gene rearrangements in patients with nodular fasciitis: a study of rare and novel USP6 fusion partners with a review of the literature

doi: 10.1136/jcp-2023-208768

Figure Lengend Snippet: Patient characteristics and clinical findings in USP6::MYH9 fusion cases

Article Snippet: Thin histological sections (3 μm thick) were used, and each sample was stained using the following antibodies and protocols: anti-smooth muscle actin (SMA) mouse monoclonal antibodies (clone 1A4, BioSB— Bioscience for the World, dilution 1:75; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath); anti-H-caldesmon mouse monoclonal antibodies (clone BSB-19|, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-desmin mouse monoclonal antibodies (clone D33, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-S100-β rabbit monoclonal antibodies (clone EP32, BioSB, dilution 1:300; pre-treatment: heating up to 99 ◦C in a pH 9 buffer in a water bath); anti-Ki-67 mouse monoclonal antibodies (clone MIB-1, BioSB, dilution 1:150; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath).

Techniques:

Journal: Frontiers in Neuroanatomy

Article Title: Cell Densities in the Mouse Brain: A Systematic Review

doi: 10.3389/fnana.2018.00083

Figure Lengend Snippet: Estimates of Cortical Density.

Article Snippet: –(Motor) , 124,000 (Schmid et al., ) 146,000 (Rockel et al., ) Mean and STD: 135,000 ± 16,000 , — , — , 13,415 GAD+ (Irintchev et al., ) 6,707 PV (Irintchev et al., ) 1,500 PV (Schmid et al., ) 3,366 CB (Irintchev et al., ) , 15,000 S-100β (Schmid et al., ) , 15,000 (Irintchev et al., ) 64,274 (Duque et al., ) Mean and STD: 39,637 ± 34,842 , 14,700 (Irintchev et al., ) .

Techniques:

Journal: Frontiers in Neuroanatomy

Article Title: Cell Densities in the Mouse Brain: A Systematic Review

doi: 10.3389/fnana.2018.00083

Figure Lengend Snippet: Cell densities for subcortical structures.

Article Snippet: –(Motor) , 124,000 (Schmid et al., ) 146,000 (Rockel et al., ) Mean and STD: 135,000 ± 16,000 , — , — , 13,415 GAD+ (Irintchev et al., ) 6,707 PV (Irintchev et al., ) 1,500 PV (Schmid et al., ) 3,366 CB (Irintchev et al., ) , 15,000 S-100β (Schmid et al., ) , 15,000 (Irintchev et al., ) 64,274 (Duque et al., ) Mean and STD: 39,637 ± 34,842 , 14,700 (Irintchev et al., ) .

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Journal: PLoS ONE

Article Title: Expression of MYCN in Multipotent Sympathoadrenal Progenitors Induces Proliferation and Neural Differentiation, but Is Not Sufficient for Tumorigenesis

doi: 10.1371/journal.pone.0133897

Figure Lengend Snippet: (A) Spheres grown from postnatal mouse adrenal glands cells express markers of neural crest stem cells (NCSCs), sympathoadrenal progenitors (SAPs), and sympathetic neurons (SNs). mRNA levels were analyzed by qRT-PCR and are shown in relation to TATA-Box binding protein-1 (TBP1). Expression data from postnatal mouse superior cervical ganglia (SCG) are included for comparison. Data are the mean values from four experiments run in triplicate. *p<0.05,**p<0.01,***p<0.001,****p < .0001, unpaired Student’s t-test. (B) The majority of sphere cells show nuclear SOX10 reactivity by immunoperoxidase labeling. Cytoplasmic nestin expression with linear morphology was observed by immunofluorescence microscopy in spheres. TH expression was prominent at the periphery of spheres. Representative images from 3 independent experiments. Scale bars equal 20 microns. (C) Sphere differentiation was induced by transfer to poly-d-lysine and fibronectin-coated plates in medium with low CEE content. Immunofluorescent images show the three cell types observed in differentiated spheres: TuJ1/TH-reactive neurons, SMA-reactive myofibroblasts, and S100-reactive Schwann cells. Scale bars 50 μM upper left panel and 25 μM all other panels. (D) A shift in differentiation potential was observed from primary spheres, grown directly from adrenal gland cells by differential plating, to secondary spheres, the products of dissociated primary spheres. Differentiated secondary spheres showed a reduced frequency of bipotent and neuron-containing colonies, and were more likely to show myofibroblast-only colonies (primary spheres n = 3, secondary spheres n = 4). Primary and secondary spheres were grown at clonal density.

Article Snippet: Primary antibodies used to assess differentiation by immunocytochemistry included those against neuronal class III β-tubulin (TUJ1)(Covance, MMS-435P, 1:400), S100 (Immunostar, 22520, 1:1), smooth muscle actin (Sigma, A5228, 1:200), and tyrosine hydroxylase (Millipore, AB152, 1:400) following block/permeabilization with 10% goat serum and 0.1% Triton X-100.

Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Comparison, Labeling, Immunofluorescence, Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: The protective and hemodynamic effects of dexmedetomidine on hypertensive cerebral hemorrhage patients in the perioperative period

doi: 10.3892/etm.2016.3711

Figure Lengend Snippet: Levels of NSE and S100β.

Article Snippet: The catecholamine (Rocky Mountain Diagnostics, Colorado Springs, CO, USA), neuron-specific enolase (NSE) and S100β (S100β protein) were estimated using ELISA as per instructions the manufacturer's (Rocky Mountain Diagnostics).

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Journal: Journal of Veterinary Internal Medicine

Article Title: Time course and prognostic value of serum GFAP, pNFH, and S100β concentrations in dogs with complete spinal cord injury because of intervertebral disc extrusion

doi: 10.1111/jvim.15439

Figure Lengend Snippet: Scatter plots of A, GFAP; B, s100β; and C, pNFH serum concentrations in 31 dogs over 14 days after onset of nonambulatory status. Dogs are identified according to whether or not they are walking at 6 months, with white squares representing dogs that did not recover and solid black circles representing dogs that did recover. GFAP, glial fibrillary acidic protein; pNFH, phosphorylated neurofilament heavy chain

Article Snippet: For S100β, an ELISA detection kit for Canine S100β Protein from MyBiosource (San Diego, California) was used.

Techniques:

Journal: Journal of Veterinary Internal Medicine

Article Title: Time course and prognostic value of serum GFAP, pNFH, and S100β concentrations in dogs with complete spinal cord injury because of intervertebral disc extrusion

doi: 10.1111/jvim.15439

Figure Lengend Snippet: Serum concentrations of GFAP, pNFH, and S100β expressed as median and range in 31 dogs categorized as walking at 6 months, yes or no and grouped as time of presentation as well as time of sampling after onset of nonambulatory status (1, 2, or 3 days). The number of samples analyzed at each time point is listed beneath the range

Article Snippet: For S100β, an ELISA detection kit for Canine S100β Protein from MyBiosource (San Diego, California) was used.

Techniques: Sampling

Journal: Journal of Veterinary Internal Medicine

Article Title: Time course and prognostic value of serum GFAP, pNFH, and S100β concentrations in dogs with complete spinal cord injury because of intervertebral disc extrusion

doi: 10.1111/jvim.15439

Figure Lengend Snippet: The estimated probability of response (ambulatory yes or no) at 6 months with the observed data for A, log average of GFAP and pNFH; B, log average of GFAP and S100β; and C, log average of pNFH and S100β overlaid. The heat map scale of probability of response ranges from 0, indicating recovery of walking, to 1, indicating no recovery of walking. White squares are dogs that did not recover; black circles are dogs that did recover. GFAP, glial fibrillary acidic protein; pNFH, phosphorylated neurofilament heavy chain

Article Snippet: For S100β, an ELISA detection kit for Canine S100β Protein from MyBiosource (San Diego, California) was used.

Techniques: