Journal: eLife

Article Title: Macrophages regulate gastrointestinal motility through complement component 1q

doi: 10.7554/eLife.78558

Figure Lengend Snippet: ( A ) Immunofluorescence analysis of enteric neuronal ganglia marked with HuC/D (red) and neuronal fibers marked with TUBB3 (green) in LMMP wholemounts of small intestines and colons from C1qa fl/fl and C1qa ∆Mφ mice. Anti-mouse IgG AlexaFluor 594 and anti-rabbit IgG AlexaFluor 488 were used as secondary stains for HuC/D and TUBB3, respectively. Images are representative of three independent experiments. Scale bars = 50 μm. ( B ) Quantification of total enteric neurons per unit area (mm 2 ) from the images shown in panel ( A ). Data are pooled from two independent experiments. Each data point represents one mouse. ( C ) Visualization of specific neuronal subsets in the LMMP from C1qa fl/fl and C1qa ∆Mφ mice by RNAscope detection. Inhibitory neurons were marked by Nos1 (green) and excitatory neurons were marked by Chat (red). Neuronal nuclei marked by HuC/D (blue) were detected by immunofluorescence. Images are representative of two independent experiments. Scale bars = 50 μm. ( D ) Immunofluorescence detection of enteric glial cells marked by S100B (green) in LMMP wholemounts from the small intestines and colons of C1qa fl/fl and C1qa ∆Mφ mice. Images are representative of two independent experiments. Scale bars = 50 μm. ( E ) qPCR analysis of Nos1 , Chat , and S100b in the LMMP of small intestines and colons from C1qa fl/fl and C1qa ∆Mφ mice. Each data point represents one mouse. Error bars represent SEM. ns, not significant by the two-tailed Student’s t -test. LMMP, longitudinal muscle-myenteric plexus.

Article Snippet: Sequence-based reagent , mouse S100b TaqMan assay , Thermo Fisher , Assay ID: Mm00485897_m1 , .

Techniques: Immunofluorescence, RNAscope, Two Tailed Test

Journal: eLife

Article Title: Macrophages regulate gastrointestinal motility through complement component 1q

doi: 10.7554/eLife.78558

Figure Lengend Snippet:

Article Snippet: Sequence-based reagent , mouse S100b TaqMan assay , Thermo Fisher , Assay ID: Mm00485897_m1 , .

Techniques: Generated, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Blocking Assay, Control, Sequencing, TaqMan Assay, RNAscope, Recombinant, Reverse Transcription, Sample Prep, SYBR Green Assay, Flow Cytometry, Plasmid Preparation, Software, Protein Extraction, Imaging, RNA Sequencing, Ex Vivo, FACS, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction

Journal: Burns & trauma

Article Title: Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury.

doi: 10.1093/burnst/tkab027

Figure Lengend Snippet: Figure 3. Ethanol is inversely correlated with neuron-specific enolase (NSE) levels but not with neurofilament light (NFL) and S100 beta (S100B) levels post-traumatic brain injury (TBI). NFL, NSE and S100B concentrations were assessed 3 hours post-TBI. Four treatment groups were used: saline–sham (SS), ethanol–sham (ES), saline–TBI (ST) and ethanol–TBI (ET). (a) Treatment groups showed significant differences in NFL concentration (p < 0.0001). Post hoc analysis showed a significant difference between SS and ST (p = 0.0005) but no difference between ST and ET (p > 0.9999). (b) Correlation analysis showed no significant relationship between NFL and ethanol in the ET group (p = 0.6188). (c) NSE concentration showed significant differences between treatment groups (p = 0.0064). Post hoc analysis revealed a significant differ- ence between SS and ST (p = 0.0057), but not between ST and ET (p > 0.9999). (d) Correlation analysis revealed a significant inverse correlation between NSE and ethanol in the ET group (p = 0.0042). (e) S100B concentration showed significant differences between treatment groups (p = 0.0282). Post hoc anal- ysis revealed a significant difference between SS and ES (p = 0.0451) and between SS and ST (p = 0.0267), but not between ST and ET (p > 0.9999). (f) Correlation analysis revealed no significant correlation between S100B and ethanol in the ET group (p = 0.9717). Boxplots represent median value, 25th to 75th percentile (box) and minimum to maximum (whiskers), including indi- vidual data points. Correlation with linear regression is shown. Group sizes: SS, n = 8; ES, n = 14; ST, n = 24; ET, n = 17. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. ns not significant

Article Snippet: Enzyme-linked immunosorbent assay determination of plasma NSE, S100B and claudin-5 Enzyme-linked immuno-sorbent assays (ELISAs) for NSE, claudin-5 and S100 beta (S100B) were performed according to the instructions of each manufacturer (mouse ENO2/NSE (CLIA) ELISA Kit, LSbio, USA; mouse claudin-5 ELISA Kit, Cusabio, USA; mouse S100 Beta ELISA Kit, LSbio, USA, respectively).

Techniques: Saline, Concentration Assay

Journal: Burns & trauma

Article Title: Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury.

doi: 10.1093/burnst/tkab027

Figure Lengend Snippet: Figure 5. Neurofilament light (NFL) levels are correlated with blood–brain barrier disruption post traumatic brain injury. Correlation analysis was performed between the concentration of NFL, neuron-specific enolase (NSE), S100 beta (S100B) and claudin-5 3 hours post-traumatic brain injury (TBI). Four treatment groups were used: saline–sham (SS), ethanol–sham (ES), saline–TBI (ST) and ethanol–TBI (ET). (a) Correlation of NFL and claudin-5 levels were significant in the SS group (p = 0.0480). (b) Correlation of NFL and claudin-5 were significant in the ST group (p = 0.0191). The other treatment groups (ES and ET) did not show this correlation. (ES: p = 0.1469; ET: R2 = 0.0136; p = 0.6785). Overall slope comparison revealed significant differences in the treatment groups between NFL and claudin-5 (F = 3.157; p = 0.0320). Post hoc analysis revealed a significant difference in the slopes between the ST and ET group (p = 0.0303). Correlation with linear regression is shown. Group sizes: SS, n = 8; ES, n = 14; ST, n = 24; ET, n = 17. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001

Article Snippet: Enzyme-linked immunosorbent assay determination of plasma NSE, S100B and claudin-5 Enzyme-linked immuno-sorbent assays (ELISAs) for NSE, claudin-5 and S100 beta (S100B) were performed according to the instructions of each manufacturer (mouse ENO2/NSE (CLIA) ELISA Kit, LSbio, USA; mouse claudin-5 ELISA Kit, Cusabio, USA; mouse S100 Beta ELISA Kit, LSbio, USA, respectively).

Techniques: Disruption, Concentration Assay, Saline, Comparison

Journal: Neuron

Article Title: Driving axon regeneration by orchestrating neuronal and non-neuronal innate immune responses via the IFNγ-cGAS-STING axis

doi: 10.1016/j.neuron.2022.10.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-S100β , Cell Signaling , Cat#9550; RRID: AB_10949319.

Techniques: Control, Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Fluorescence, Reverse Transcription, SYBR Green Assay, Single Cell Gel Electrophoresis, Knock-Out, Knock-In, shRNA, Sequencing, Software

Journal: bioRxiv

Article Title: Transcriptomic heterogeneity of Sox2 -expressing pituitary cells

doi: 10.1101/2021.12.10.472137

Figure Lengend Snippet: S100 gene expression in the pituitary gland. (a) UMAP scatter plots showing expression of S100b , common to FS and Pc, S100a6 , expressed in FS and Pe, and S100g , expressed in Pc and a subset of S and L. (b) Dot plot indicating the mean expression of S100 genes in each cell type, with EC and Pe divided by lobe of origin and FSC divided by subtypes. (c) qRT-PCR measurements of S100 gene expression in dispersed cells and pituitary tissue from 44-day old female rats. Solid line – linear fit; dotted lines – 95% confidence intervals. (d, e) Immunofluorescence analysis of S100A and S100B proteins in primary cultured pituitary cells. (d) S100A expression in cells from females (left) and S100B expression in cells from females (center) and males (right). (e) Colocalization of S100A (red, left) and S100B (green, center), with overlay showing dual labeled cells (right). In 6 separate replicates, a total of 494 cells were S100A+, 339 were S100B+, and 275 were dual labeled. Cell nuclei are stained with DAPI (blue). Arrows indicate an example cell that co-expresses both proteins. Scale bars (applies to all images), 10 µm.

Article Snippet: Applied Biosystems predesigned TaqMan Gene Expression Assays were used: Aldh1a1 (Rn00755484_m1), Aldh1l1 (Rn00674034_m1), Aqp4 (Rn01401327_s1), Edn3 (Rn01755284_m1), Fibin (Rn01514386_s1), Gapdh (Rn01462662_g1), Gfap (Rn01253033_m1), Gstm2 (Rn00598597_m1), Maob (Rn00566203_m1), Nes (Rn01455599_g1), Penk (Rn00567566_m1), S100a1 (Rn01458753_m1), S100a6 (Rn00821474_g1), S100a10 (Rn06378613_s1), S100a11 (Rn01409258_g1), S100a13 (Rn01769833_m1), S100a16 (Rn01458849_g1), S100b (Rn04219408_m1), Slc1a2 (Rn00691548_m1), Slc1a3 (Rn01402419_g1), Sox2 (Rn01286286_g1), Sox9 (Rn01751070_m1), Sult1a1 (Rn01510633_m1), and Vim (Rn00667825_m1).

Techniques: Gene Expression, Expressing, Quantitative RT-PCR, Immunofluorescence, Cell Culture, Labeling, Staining