s100a8 protein Search Results


recombinant s100a8  (MedChemExpress)
94
MedChemExpress recombinant s100a8
( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic <t>S100a8</t> / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).
Recombinant S100a8, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant s100a8/product/MedChemExpress
Average 94 stars, based on 1 article reviews
recombinant s100a8 - by Bioz Stars, 2026-05
94/100 stars
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recombinant mouse s100a8 a9  (R&D Systems)
93
R&D Systems recombinant mouse s100a8 a9
( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic <t>S100a8</t> / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).
Recombinant Mouse S100a8 A9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse s100a8 a9/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant mouse s100a8 a9 - by Bioz Stars, 2026-05
93/100 stars
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recombinant human rh s100a8 protein  (R&D Systems)
90
R&D Systems recombinant human rh s100a8 protein
( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic <t>S100a8</t> / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).
Recombinant Human Rh S100a8 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human rh s100a8 protein/product/R&D Systems
Average 90 stars, based on 1 article reviews
recombinant human rh s100a8 protein - by Bioz Stars, 2026-05
90/100 stars
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recombinant human heterodimer mrp8 14 proteins  (R&D Systems)
93
R&D Systems recombinant human heterodimer mrp8 14 proteins
( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic <t>S100a8</t> / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).
Recombinant Human Heterodimer Mrp8 14 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human heterodimer mrp8 14 proteins/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant human heterodimer mrp8 14 proteins - by Bioz Stars, 2026-05
93/100 stars
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polyclonal antibody against s100a8  (Proteintech)
95
Proteintech polyclonal antibody against s100a8
( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic <t>S100a8</t> / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).
Polyclonal Antibody Against S100a8, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibody against s100a8/product/Proteintech
Average 95 stars, based on 1 article reviews
polyclonal antibody against s100a8 - by Bioz Stars, 2026-05
95/100 stars
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recombinant mouse s100a8  (R&D Systems)
93
R&D Systems recombinant mouse s100a8
In vivo PMN chemotactic ability of culture supernatants containing C. albicans-derived S100 alarmins. (A) PMN migration induced by S100 alarmins of C. albicans or E. coli origin. Overnight culture supernatants of <t>S100A8,</t> S100A9, and wild-type C. albicans were semisolidified with carboxymethylcellulose (3%) to reach a consistency of vaginal gel formulation. The vaginal gel preparations were administered intravaginally to estrogenized uninoculated mice once daily for 2 days in a volume of 20 μl per mouse using a microdispenser. Gel preparations of E. coli-derived recombinant <t>S100A8</t> (rS100A8), S100A9 (rS100A9), or PBS (gel control) were tested in parallel. Vaginal lavage samples were collected prior to treatment and then daily after the last treatment. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (×400 magnification) per mouse, and values were averaged. (B) Dose-dependent effects of S100A8-containing culture supernatant in vaginal PMN migration. Estrogenized uninoculated mice were treated with culture supernatant from S100A8-producing C. albicans at various dilutions. Vaginal lavage fluids were evaluated for PMNs at 48 h postadministration. The figure presents cumulative data from two repeats. *, P < 0.05 compared to the estrogenized untreated group (0 h). SEM, standard error of the mean.
Recombinant Mouse S100a8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse s100a8/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant mouse s100a8 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
recombinant mouse s100a8 s100a9 proteins  (R&D Systems)
94
R&D Systems recombinant mouse s100a8 s100a9 proteins
In vivo PMN chemotactic ability of culture supernatants containing C. albicans-derived S100 alarmins. (A) PMN migration induced by S100 alarmins of C. albicans or E. coli origin. Overnight culture supernatants of <t>S100A8,</t> S100A9, and wild-type C. albicans were semisolidified with carboxymethylcellulose (3%) to reach a consistency of vaginal gel formulation. The vaginal gel preparations were administered intravaginally to estrogenized uninoculated mice once daily for 2 days in a volume of 20 μl per mouse using a microdispenser. Gel preparations of E. coli-derived recombinant <t>S100A8</t> (rS100A8), S100A9 (rS100A9), or PBS (gel control) were tested in parallel. Vaginal lavage samples were collected prior to treatment and then daily after the last treatment. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (×400 magnification) per mouse, and values were averaged. (B) Dose-dependent effects of S100A8-containing culture supernatant in vaginal PMN migration. Estrogenized uninoculated mice were treated with culture supernatant from S100A8-producing C. albicans at various dilutions. Vaginal lavage fluids were evaluated for PMNs at 48 h postadministration. The figure presents cumulative data from two repeats. *, P < 0.05 compared to the estrogenized untreated group (0 h). SEM, standard error of the mean.
Recombinant Mouse S100a8 S100a9 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse s100a8 s100a9 proteins/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant mouse s100a8 s100a9 proteins - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
s100a8 a9 recombinant protein  (R&D Systems)
94
R&D Systems s100a8 a9 recombinant protein
( A ) UMAP plot showing six distinct cell types in ovarian tissue. ( B ) Comparison of pyroptosis-related gene scores among different cell types in ovarian tissue ( n = 3). ( C ) Distribution of <t>S100A8</t> and S100A9 gene expression in immune cells of ovarian tissue ( n = 3). ( D ) Comparison of S100a8 and S100a9 gene expression in immune cells of ovarian tissue between two groups of mice ( n = 3). ( E ) Measurement <t>of</t> <t>S100A8/A9</t> levels in peripheral blood serum using ELISA ( n = 3). ( F ) Measurement of S100A8/A9 levels in ovarian tissue using ELISA ( n = 3). ( G ) Comparison of macrophage M1 polarization–related gene set scores in ovarian tissue between the two groups of mice. Larger dots represent a higher number of differential genes, and darker colors indicate larger gene expression differences. Data are presented as mean ± SEM. *** P < 0.001. The P value was calculated using a one-tailed unpaired Student’s t test. One-way ANOVA for multiple group comparisons, with Tukey’s post hoc test used for post hoc analyses.
S100a8 A9 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s100a8 a9 recombinant protein/product/R&D Systems
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s100a8 a9 recombinant protein - by Bioz Stars, 2026-05
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human s100a8  (Elabscience Biotechnology)
92
Elabscience Biotechnology human s100a8
( A ) UMAP plot showing six distinct cell types in ovarian tissue. ( B ) Comparison of pyroptosis-related gene scores among different cell types in ovarian tissue ( n = 3). ( C ) Distribution of <t>S100A8</t> and S100A9 gene expression in immune cells of ovarian tissue ( n = 3). ( D ) Comparison of S100a8 and S100a9 gene expression in immune cells of ovarian tissue between two groups of mice ( n = 3). ( E ) Measurement <t>of</t> <t>S100A8/A9</t> levels in peripheral blood serum using ELISA ( n = 3). ( F ) Measurement of S100A8/A9 levels in ovarian tissue using ELISA ( n = 3). ( G ) Comparison of macrophage M1 polarization–related gene set scores in ovarian tissue between the two groups of mice. Larger dots represent a higher number of differential genes, and darker colors indicate larger gene expression differences. Data are presented as mean ± SEM. *** P < 0.001. The P value was calculated using a one-tailed unpaired Student’s t test. One-way ANOVA for multiple group comparisons, with Tukey’s post hoc test used for post hoc analyses.
Human S100a8, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human s100a8/product/Elabscience Biotechnology
Average 92 stars, based on 1 article reviews
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recombinant human s100a1 protein  (Beijing Solarbio Science)
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Beijing Solarbio Science recombinant human s100a1 protein
( A ) UMAP plot showing six distinct cell types in ovarian tissue. ( B ) Comparison of pyroptosis-related gene scores among different cell types in ovarian tissue ( n = 3). ( C ) Distribution of <t>S100A8</t> and S100A9 gene expression in immune cells of ovarian tissue ( n = 3). ( D ) Comparison of S100a8 and S100a9 gene expression in immune cells of ovarian tissue between two groups of mice ( n = 3). ( E ) Measurement <t>of</t> <t>S100A8/A9</t> levels in peripheral blood serum using ELISA ( n = 3). ( F ) Measurement of S100A8/A9 levels in ovarian tissue using ELISA ( n = 3). ( G ) Comparison of macrophage M1 polarization–related gene set scores in ovarian tissue between the two groups of mice. Larger dots represent a higher number of differential genes, and darker colors indicate larger gene expression differences. Data are presented as mean ± SEM. *** P < 0.001. The P value was calculated using a one-tailed unpaired Student’s t test. One-way ANOVA for multiple group comparisons, with Tukey’s post hoc test used for post hoc analyses.
Recombinant Human S100a1 Protein, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human s100a1 protein/product/Beijing Solarbio Science
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rs100a8  (MedChemExpress)
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MedChemExpress rs100a8
CEBPB transcriptionally activates S100A8 to promote M2 macrophage polarization. A , Venn diagram of three datasets identifying four common secretory factors: differentially expressed genes from CEBPB-overexpressing 4T07 cells bulk RNA-seq, CEBPB binding peaks in 4T07 cells from anti-CEBPB ChIP-seq, and the profile of mouse secretory proteins. B , CEBPB binding peaks for S100A8 and Cxcl1 from anti-CEBPB ChIP-seq in 4T07 cells. C , ChIP-qPCR showing the CEBPB binding regions in the S100a8 promoter or upstream regions of Cxcl1 of 4T07 and EMT6 cells. D , Relative luciferase activity in CEBPB-overexpressing HEK293T cells with the S100a8 promoter WT or MUT, respectively. E-F , mRNA expression of S100a8 or Cxcl1 in 4T07 cells with CEBPB overexpression (E) or knockdown (F). G , Protein expression of S100A8 in 4T07 cells with or without CEBPB overexpression. H , Representative multiplex immunohistochemistry images of Ki67, CEBPB, and S100A8 staining in a TMA of TNBC patient tumor smaples ( n = 80). I , mRNA expression of S100a8 in eGFP -/lo and eGFP +/hi tumor cells sorted from 4T07-TetOn or EMT6-TetOn tumor masses. J-K , Percentages of CD206 (J) and MHCII (K) expressing BMDMs with the addition of <t>rS100A8</t> (100 or 500 ng/mL) or rCXCL1 (100 or 500 ng/mL) for 48 hours. L-N , Tumor volume (L), image (M) and weight (N) of CEBPB-overexpressing with or without S100A8 knockdown EMT6 allografts in BALB/c mice ( n = 5). O-R , Flow cytometry analysis of CD8 + T cells (O), CD11b + F4/80 + cells (P), CD206 + macrophages (Q), and MHCII + macrophages (R) infiltrated in tumors developed in (L). n.s., not significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001.
Rs100a8, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rs100a8/product/MedChemExpress
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( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic S100a8 / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).

Journal: The Journal of Clinical Investigation

Article Title: Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii –derived glutamic acid–maintained Tregs

doi: 10.1172/JCI196788

Figure Lengend Snippet: ( A ) Fecal microbiota from Clec4n –/– mice were cultured on MRS medium supplemented with neomycin for 3 days. Single colonies were isolated and identified by PCR amplification and sequencing of bacterial 16S rDNA. ( B – F ) L.j . WXY strain was isolated from mouse feces. WT mice were treated with antibiotics (ABX), followed by oral transfer of the indicated bacterial strains twice over 7 days. Whole fecal microbiota from normal WT mice were then transferred back for 3 days, followed by DSS treatment for 7 days and sacrifice on day 10. Body weight loss ( B ), DAI ( C ), colon length measurement ( D ), distal colon histology ( E ), and cLP neutrophil and Treg frequencies ( F ) ( B – D , and F , PBS, n = 7; L . j . WXY, n = 7; Lactobacillus mixture, n = 8; E . coli , n = 7; E , n = 3 /group). ( G ) Correlation between fecal Lactobacillus abundance and colonic S100a8 / S100a9 mRNA expression in C57BL/6J mice under steady-state conditions ( n = 18). ( H ) L.j . WXY was cultured anaerobically at 37°C with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each). Bacterial growth was quantified spectrophotometrically at 6 and 24 hours ( n = 6 technical replicates/group). ( I ) Clec4n –/– mice received intrarectal administration of recombinant S100A8 + S100A9 for 5 hours, followed by quantification of fecal L.j . abundance by qPCR ( n = 10). ( J and K ) WT and Clec4n –/– recipient mice were lethally irradiated and reconstituted with BM cells from WT or Clec4n –/– donors. After 30 days, colonic expression of S100a8 and S100a9 was assessed by qPCR ( J ), and fecal Lactobacillus abundance was measured by qPCR on days 10, 20, and 30 after transfer ( K ) (WT→WT, n = 8; WT→ Clec4n –/– , n = 7; Clec4n –/– →WT, n = 8; Clec4n –/– → Clec4n –/– , n = 8.). ( L – N ) WT and Clec4n –/– mice were treated intraperitoneally with anti-Ly6G neutralizing Ab or isotype control IgG (100 μg/mouse) every other day for 5 doses. Two days after the final injection, colonic tissues and feces were collected. S100A8 and S100A9 protein levels in colon lysates were measured by ELISA ( L ), fecal L.j . abundance was determined by qPCR ( M ), and colonic Il6 and Tnf expression was assessed by qPCR ( N ) (control [con] IgG, n = 3–4; anti-Ly6G, n = 3–4). ( O – S ) WT mice were orally administered culture supernatant (sup.) from L.j . WXY or heat-killed (hk) bacteria daily for 3 days before and throughout 7 days of DSS treatment ( n = 10 total administrations). Body weight loss ( O ), DAI ( P ), colon length measurement at sacrifice on day 9 ( Q ), distal colon histology ( R ), and cLP Treg frequencies ( S ) (PBS n = 8; WXY-sup. n = 8; WXY-hk n = 9). ( T ) CD11b + and CD11c + cells isolated from WT cLP were stimulated in vitro with L.j . WXY, Lactobacillus mixture, or E . coli . After 12 hours, Il10 and Tgfb1 mRNA expression was quantified by qPCR ( n = 4 technical replicates/group). ( U ) CD62L + naive CD4 + T cells isolated from WT spleen and lymph nodes were polarized toward Treg differentiation in the presence of L.j . WXY culture supernatant. After 6 days, Il10 and Tgfb1 expression was measured by qPCR ( n = 9 technical replicates from 3 biological replicates/group). ( V and W ) ABX-treated WT mice received oral L.j . WXY or PBS, followed by fecal microbiota transplantation from normal WT mice and subsequent DSS treatment. Cecal contents were collected on day 10 for untargeted metabolomic analysis by liquid chromatography. Histogram ( V ) shows the top 20 enriched metabolites, and volcano plot ( W ) highlights significantly altered metabolites in WXY-treated mice. ( X ) Targeted metabolomic analysis of l -glutamic acid levels in cecal contents from 8-week-old WT and Clec4n –/– mice under physiological conditions ( n = 3/group). ( Y ) l -Glutamic acid concentrations in culture supernatants of L.j . WXY, Lactobacillus mixture, or E . coli measured by targeted metabolomics ( n = 3 replicates/group). Data in B – F , H – K , and O – S are pooled from 2 independent experiments. Data in B – F , J , L – U , X , and Y are presented as mean ± SD. Statistical analysis: 1-way ANOVA with Bonferroni’s multiple-comparison test ( B , C , K , O , and P ); 1-way ANOVA with Tukey’s multiple-comparison test ( D – F , J , L – N , Q – T , and Y ); Spearman’s correlation test ( G ); 2-way ANOVA test with repeated measures ( H ); paired Student’s t test ( I ); or 2-tailed unpaired Student’s t test ( U and X ).

Article Snippet: Lactobacillus cultures were incubated in MRS medium containing a 1:1 mixture of recombinant S100A8 and S100A9 peptides (5 μg/mL each; HY-P71076, MCE) for 6 or 24 hours under anaerobic conditions.

Techniques: Cell Culture, Isolation, Amplification, Sequencing, Expressing, Recombinant, Irradiation, Control, Injection, Enzyme-linked Immunosorbent Assay, Bacteria, In Vitro, Transplantation Assay, Metabolomic, Liquid Chromatography, Comparison

( A ) Phylum- and genus-level composition of human fecal commensal fungi isolated using Dectin-2–Fc and identified by qPCR with species-specific primers. ( B ) Relative contents of Engyodontium sp. in fecal fungi and L.j . in fecal bacteria from patients with CD and healthy individuals (HC), determined by qPCR ( Engyodontium : HC, n = 59; CD, n = 62. Lacutobacillus : HC, n = 62; CD, n = 63). ( C ) Relative contents of Engyodontium sp. in fecal fungi and L.j . in fecal bacteria from patients with UC and healthy individuals, determined by qPCR (HC, n = 36; UC, n = 25). ( D ) Correlation between Ct value of L.j . and Engyodontium sp. from human individuals as described in B ( n = 97 [including HC, n = 48 and CD, n = 49]). ( E ) Correlation between Ct value of L.j . and Engyodontium sp. from human individuals as described in C (n=61 [including HC, n=36 and UC, n=25]). ( F ) C57BL/6J SPF mice were orally colonized with Engyodontium sp. ( Engyod. ) every other day for 3 administrations. Thirteen days later, fecal samples were collected, and the relative abundance of fecal L.j . was quantified by qPCR ( n = 10 /group). ( G ) Relative expression of CLEC6A , S100A8 , and S100A9 in colon tissues from patients with CD and healthy control (HC) individuals, determined by bulk RNA-Seq analysis (HC, n = 47; CD, n = 57). ( H ) Correlation between CLEC6A and S100A8 or S100A9 expression based on bulk RNA-Seq data described in G . ( I ) Correlation between CLEC6A and S100A8 or S100A9 relative expression in colon tissues of patients with UC, determined by qPCR ( n = 25). ( J ) IHC staining for DECTIN-2 and S100A8/S100A9 proteins in serial inflammatory colon sections obtained from a patient with CD after surgical resection. ( K ) IHC staining for human DECTIN-2 in paired normal and inflamed colon regions from the patient with CD shown in J . ( L ) Transcriptional levels of CLEC6A in colon tissues from non-IBD control individuals and patients with colonic CD (cCD), ileal CD (iCD), or UC, analyzed by reprocessing a public bulk RNA-Seq dataset ( GSE117993 ) (not IBD, n = 55; cCD, n = 31; iCD, n = 60; UC, n = 43). ( M ) Colon tissues from 3 patients with IBD were obtained after resection. cLP CD11b + cells were isolated and stimulated with α-mannan in vitro for 6 hours. S100A8 and S100A9 relative expression was quantified by qPCR ( n = 7). ( N ) Neutrophils isolated from peripheral blood of 2 patients with IBD were stimulated with α-mannan in vitro for 6 hours, and S100A8 and S100A9 relative expression was measured by qPCR ( n = 7 technical replicates from 2 biological replicates/group). ( O ) Correlation between fecal Engyodontium sp. abundance and colonic expression of CLEC6A , S100A8 , or S100A9 in patients with UC ( n = 25). ( P ) Correlation between fecal L.j . abundance and colonic expression of CLEC6A , S100A8 , or S100A9 in patients with UC ( n = 25). ( Q ) CD11b + cells isolated from cLP of resected colon tissues from 2 patients with IBD were stimulated with Engyodontium sp. for 24 hours in vitro, followed by qPCR analysis of S100A8 and S100A9 expression ( n = 6 technical replicates from 2 biological replicates/group). non-stimu., nonstimulated. ( R ) Engyodontium sp. was cultured at room temperature with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each) for 12, 24, and 48 hours. Fungus growth was quantified by CFU enumeration on PDA plates ( n = 3 replicates/group). ( S ) Relative amount of glutamic acid in fecal samples from patients with CD and healthy control individuals, assessed by liquid chromatography for untargeted metabolomics (HC, n = 80; CD, n = 65). ( T ) Correlation between 1/Ct value of L.j . and fecal glutamic acid levels in patients with CD ( n = 8). ( U ) Correlation between 1/Ct value of L.j . and glutamic acid concentration in colon tissues from patients with UC ( n = 25). ( V ) Peripheral blood leukocytes from 2 healthy donors were isolated and induced toward Treg differentiation in the presence of l -glutamic acid. After 5 days, the proportion of Foxp3 + CD4 + among CD45 + leukocytes was analyzed by flow cytometry ( n = 6 technical replicates from 2 biological replicates/group). Data in F , M , N , Q , and V are pooled from 2 independent experiments. Data in B , C , G , and L are presented as mean ± SEM, and in F , N , Q , R , S , and V as mean ± SD. Statistical analysis: 2-tailed Mann-Whitney test ( B , C , and G ), 2-tailed unpaired Student’s t test ( F , N , Q – S , and V ), 1-way ANOVA with Kruskal-Wallis and Dunn’s test ( L ), Spearman’s correlation test ( D , E , H , I , O , P , T , and U ), or paired Student’s t test ( M ).

Journal: The Journal of Clinical Investigation

Article Title: Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii –derived glutamic acid–maintained Tregs

doi: 10.1172/JCI196788

Figure Lengend Snippet: ( A ) Phylum- and genus-level composition of human fecal commensal fungi isolated using Dectin-2–Fc and identified by qPCR with species-specific primers. ( B ) Relative contents of Engyodontium sp. in fecal fungi and L.j . in fecal bacteria from patients with CD and healthy individuals (HC), determined by qPCR ( Engyodontium : HC, n = 59; CD, n = 62. Lacutobacillus : HC, n = 62; CD, n = 63). ( C ) Relative contents of Engyodontium sp. in fecal fungi and L.j . in fecal bacteria from patients with UC and healthy individuals, determined by qPCR (HC, n = 36; UC, n = 25). ( D ) Correlation between Ct value of L.j . and Engyodontium sp. from human individuals as described in B ( n = 97 [including HC, n = 48 and CD, n = 49]). ( E ) Correlation between Ct value of L.j . and Engyodontium sp. from human individuals as described in C (n=61 [including HC, n=36 and UC, n=25]). ( F ) C57BL/6J SPF mice were orally colonized with Engyodontium sp. ( Engyod. ) every other day for 3 administrations. Thirteen days later, fecal samples were collected, and the relative abundance of fecal L.j . was quantified by qPCR ( n = 10 /group). ( G ) Relative expression of CLEC6A , S100A8 , and S100A9 in colon tissues from patients with CD and healthy control (HC) individuals, determined by bulk RNA-Seq analysis (HC, n = 47; CD, n = 57). ( H ) Correlation between CLEC6A and S100A8 or S100A9 expression based on bulk RNA-Seq data described in G . ( I ) Correlation between CLEC6A and S100A8 or S100A9 relative expression in colon tissues of patients with UC, determined by qPCR ( n = 25). ( J ) IHC staining for DECTIN-2 and S100A8/S100A9 proteins in serial inflammatory colon sections obtained from a patient with CD after surgical resection. ( K ) IHC staining for human DECTIN-2 in paired normal and inflamed colon regions from the patient with CD shown in J . ( L ) Transcriptional levels of CLEC6A in colon tissues from non-IBD control individuals and patients with colonic CD (cCD), ileal CD (iCD), or UC, analyzed by reprocessing a public bulk RNA-Seq dataset ( GSE117993 ) (not IBD, n = 55; cCD, n = 31; iCD, n = 60; UC, n = 43). ( M ) Colon tissues from 3 patients with IBD were obtained after resection. cLP CD11b + cells were isolated and stimulated with α-mannan in vitro for 6 hours. S100A8 and S100A9 relative expression was quantified by qPCR ( n = 7). ( N ) Neutrophils isolated from peripheral blood of 2 patients with IBD were stimulated with α-mannan in vitro for 6 hours, and S100A8 and S100A9 relative expression was measured by qPCR ( n = 7 technical replicates from 2 biological replicates/group). ( O ) Correlation between fecal Engyodontium sp. abundance and colonic expression of CLEC6A , S100A8 , or S100A9 in patients with UC ( n = 25). ( P ) Correlation between fecal L.j . abundance and colonic expression of CLEC6A , S100A8 , or S100A9 in patients with UC ( n = 25). ( Q ) CD11b + cells isolated from cLP of resected colon tissues from 2 patients with IBD were stimulated with Engyodontium sp. for 24 hours in vitro, followed by qPCR analysis of S100A8 and S100A9 expression ( n = 6 technical replicates from 2 biological replicates/group). non-stimu., nonstimulated. ( R ) Engyodontium sp. was cultured at room temperature with recombinant S100A8 + S100A9 (1:1 mixture, 5 μg/mL each) for 12, 24, and 48 hours. Fungus growth was quantified by CFU enumeration on PDA plates ( n = 3 replicates/group). ( S ) Relative amount of glutamic acid in fecal samples from patients with CD and healthy control individuals, assessed by liquid chromatography for untargeted metabolomics (HC, n = 80; CD, n = 65). ( T ) Correlation between 1/Ct value of L.j . and fecal glutamic acid levels in patients with CD ( n = 8). ( U ) Correlation between 1/Ct value of L.j . and glutamic acid concentration in colon tissues from patients with UC ( n = 25). ( V ) Peripheral blood leukocytes from 2 healthy donors were isolated and induced toward Treg differentiation in the presence of l -glutamic acid. After 5 days, the proportion of Foxp3 + CD4 + among CD45 + leukocytes was analyzed by flow cytometry ( n = 6 technical replicates from 2 biological replicates/group). Data in F , M , N , Q , and V are pooled from 2 independent experiments. Data in B , C , G , and L are presented as mean ± SEM, and in F , N , Q , R , S , and V as mean ± SD. Statistical analysis: 2-tailed Mann-Whitney test ( B , C , and G ), 2-tailed unpaired Student’s t test ( F , N , Q – S , and V ), 1-way ANOVA with Kruskal-Wallis and Dunn’s test ( L ), Spearman’s correlation test ( D , E , H , I , O , P , T , and U ), or paired Student’s t test ( M ).

Article Snippet: Lactobacillus cultures were incubated in MRS medium containing a 1:1 mixture of recombinant S100A8 and S100A9 peptides (5 μg/mL each; HY-P71076, MCE) for 6 or 24 hours under anaerobic conditions.

Techniques: Isolation, Bacteria, Expressing, Control, RNA Sequencing, Immunohistochemistry, In Vitro, Cell Culture, Recombinant, Liquid Chromatography, Concentration Assay, Flow Cytometry, MANN-WHITNEY

In vivo PMN chemotactic ability of culture supernatants containing C. albicans-derived S100 alarmins. (A) PMN migration induced by S100 alarmins of C. albicans or E. coli origin. Overnight culture supernatants of S100A8, S100A9, and wild-type C. albicans were semisolidified with carboxymethylcellulose (3%) to reach a consistency of vaginal gel formulation. The vaginal gel preparations were administered intravaginally to estrogenized uninoculated mice once daily for 2 days in a volume of 20 μl per mouse using a microdispenser. Gel preparations of E. coli-derived recombinant S100A8 (rS100A8), S100A9 (rS100A9), or PBS (gel control) were tested in parallel. Vaginal lavage samples were collected prior to treatment and then daily after the last treatment. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (×400 magnification) per mouse, and values were averaged. (B) Dose-dependent effects of S100A8-containing culture supernatant in vaginal PMN migration. Estrogenized uninoculated mice were treated with culture supernatant from S100A8-producing C. albicans at various dilutions. Vaginal lavage fluids were evaluated for PMNs at 48 h postadministration. The figure presents cumulative data from two repeats. *, P < 0.05 compared to the estrogenized untreated group (0 h). SEM, standard error of the mean.

Journal: Infection and Immunity

Article Title: Vaginal Epithelial Cell-Derived S100 Alarmins Induced by Candida albicans via Pattern Recognition Receptor Interactions Are Sufficient but Not Necessary for the Acute Neutrophil Response during Experimental Vaginal Candidiasis

doi: 10.1128/IAI.00861-13

Figure Lengend Snippet: In vivo PMN chemotactic ability of culture supernatants containing C. albicans-derived S100 alarmins. (A) PMN migration induced by S100 alarmins of C. albicans or E. coli origin. Overnight culture supernatants of S100A8, S100A9, and wild-type C. albicans were semisolidified with carboxymethylcellulose (3%) to reach a consistency of vaginal gel formulation. The vaginal gel preparations were administered intravaginally to estrogenized uninoculated mice once daily for 2 days in a volume of 20 μl per mouse using a microdispenser. Gel preparations of E. coli-derived recombinant S100A8 (rS100A8), S100A9 (rS100A9), or PBS (gel control) were tested in parallel. Vaginal lavage samples were collected prior to treatment and then daily after the last treatment. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (×400 magnification) per mouse, and values were averaged. (B) Dose-dependent effects of S100A8-containing culture supernatant in vaginal PMN migration. Estrogenized uninoculated mice were treated with culture supernatant from S100A8-producing C. albicans at various dilutions. Vaginal lavage fluids were evaluated for PMNs at 48 h postadministration. The figure presents cumulative data from two repeats. *, P < 0.05 compared to the estrogenized untreated group (0 h). SEM, standard error of the mean.

Article Snippet: Recombinant mouse S100A8 and S100A9 ( Escherichia coli -derived; available by custom order from R&D Systems) or culture supernatants containing C. albicans -derived S100A8 or S100A9 were intravaginally administered to estrogenized mice.

Techniques: In Vivo, Derivative Assay, Migration, Formulation, Recombinant, Control, Staining

Antibody neutralization of S100A8 and S100A9 during infection. Estrogenized inoculated mice were intravaginally treated with anti-S100A8 (100 μg/ml), anti-S100A9 (100 μg/ml), or a combination of the two antibodies in a volume of 20 μl on days 1, 2, and 3 postinoculation. Vaginal lavage samples were collected prior to inoculation and on days 4 and 7 postinoculation. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (magnification, ×400) per mouse and averaged. The figure presents cumulative data from three repeats with 5 mice per group. SEM, standard error of the mean.

Journal: Infection and Immunity

Article Title: Vaginal Epithelial Cell-Derived S100 Alarmins Induced by Candida albicans via Pattern Recognition Receptor Interactions Are Sufficient but Not Necessary for the Acute Neutrophil Response during Experimental Vaginal Candidiasis

doi: 10.1128/IAI.00861-13

Figure Lengend Snippet: Antibody neutralization of S100A8 and S100A9 during infection. Estrogenized inoculated mice were intravaginally treated with anti-S100A8 (100 μg/ml), anti-S100A9 (100 μg/ml), or a combination of the two antibodies in a volume of 20 μl on days 1, 2, and 3 postinoculation. Vaginal lavage samples were collected prior to inoculation and on days 4 and 7 postinoculation. Vaginal PMNs were quantified by identifying PMNs by Pap smear staining and enumerating PMNs in 5 high-powered fields (magnification, ×400) per mouse and averaged. The figure presents cumulative data from three repeats with 5 mice per group. SEM, standard error of the mean.

Article Snippet: Recombinant mouse S100A8 and S100A9 ( Escherichia coli -derived; available by custom order from R&D Systems) or culture supernatants containing C. albicans -derived S100A8 or S100A9 were intravaginally administered to estrogenized mice.

Techniques: Neutralization, Infection, Staining

Role of S100 alarmins in the PMN response during infection. Quantification of vaginal Candida burden (A) and vaginal PMNs (B). The number of CFU per 100 μl of lavage fluids from estrogenized S100A8- and S100A9-deficient or C57BL/6 wild-type mice inoculated with C. albicans was assessed on day 4 postinoculation. PMNs in vaginal lavage fluid were identified by Pap smear staining and enumerated in 5 high-powered fields (magnification, ×400) per mouse. The figure presents cumulative data from three repeats with 10 mice per group. ***, P < 0.0001 compared to the wild-type control group. SEM, standard error of the mean.

Journal: Infection and Immunity

Article Title: Vaginal Epithelial Cell-Derived S100 Alarmins Induced by Candida albicans via Pattern Recognition Receptor Interactions Are Sufficient but Not Necessary for the Acute Neutrophil Response during Experimental Vaginal Candidiasis

doi: 10.1128/IAI.00861-13

Figure Lengend Snippet: Role of S100 alarmins in the PMN response during infection. Quantification of vaginal Candida burden (A) and vaginal PMNs (B). The number of CFU per 100 μl of lavage fluids from estrogenized S100A8- and S100A9-deficient or C57BL/6 wild-type mice inoculated with C. albicans was assessed on day 4 postinoculation. PMNs in vaginal lavage fluid were identified by Pap smear staining and enumerated in 5 high-powered fields (magnification, ×400) per mouse. The figure presents cumulative data from three repeats with 10 mice per group. ***, P < 0.0001 compared to the wild-type control group. SEM, standard error of the mean.

Article Snippet: Recombinant mouse S100A8 and S100A9 ( Escherichia coli -derived; available by custom order from R&D Systems) or culture supernatants containing C. albicans -derived S100A8 or S100A9 were intravaginally administered to estrogenized mice.

Techniques: Infection, Staining, Control

In vitro screening of S100 alarmin response by PRR-deficient vaginal epithelial cells. Primary vaginal epithelial cell cultures derived from wild-type, MR−/−, TLR4-deficient, and SIGNR1−/− vaginal tissue explants were inoculated with C. albicans, and S100A8 (A) and S100A9 (B) mRNA expression was quantified by real-time PCR 24 h following inoculation. The expression of the target genes was normalized to that of the β-actin gene and expressed as the fold increase over expression in epithelial cells cultured alone in parallel. The figure presents cumulative data from two repeats. SEM, standard error of the mean.

Journal: Infection and Immunity

Article Title: Vaginal Epithelial Cell-Derived S100 Alarmins Induced by Candida albicans via Pattern Recognition Receptor Interactions Are Sufficient but Not Necessary for the Acute Neutrophil Response during Experimental Vaginal Candidiasis

doi: 10.1128/IAI.00861-13

Figure Lengend Snippet: In vitro screening of S100 alarmin response by PRR-deficient vaginal epithelial cells. Primary vaginal epithelial cell cultures derived from wild-type, MR−/−, TLR4-deficient, and SIGNR1−/− vaginal tissue explants were inoculated with C. albicans, and S100A8 (A) and S100A9 (B) mRNA expression was quantified by real-time PCR 24 h following inoculation. The expression of the target genes was normalized to that of the β-actin gene and expressed as the fold increase over expression in epithelial cells cultured alone in parallel. The figure presents cumulative data from two repeats. SEM, standard error of the mean.

Article Snippet: Recombinant mouse S100A8 and S100A9 ( Escherichia coli -derived; available by custom order from R&D Systems) or culture supernatants containing C. albicans -derived S100A8 or S100A9 were intravaginally administered to estrogenized mice.

Techniques: In Vitro, Derivative Assay, Expressing, Real-time Polymerase Chain Reaction, Over Expression, Cell Culture

( A ) UMAP plot showing six distinct cell types in ovarian tissue. ( B ) Comparison of pyroptosis-related gene scores among different cell types in ovarian tissue ( n = 3). ( C ) Distribution of S100A8 and S100A9 gene expression in immune cells of ovarian tissue ( n = 3). ( D ) Comparison of S100a8 and S100a9 gene expression in immune cells of ovarian tissue between two groups of mice ( n = 3). ( E ) Measurement of S100A8/A9 levels in peripheral blood serum using ELISA ( n = 3). ( F ) Measurement of S100A8/A9 levels in ovarian tissue using ELISA ( n = 3). ( G ) Comparison of macrophage M1 polarization–related gene set scores in ovarian tissue between the two groups of mice. Larger dots represent a higher number of differential genes, and darker colors indicate larger gene expression differences. Data are presented as mean ± SEM. *** P < 0.001. The P value was calculated using a one-tailed unpaired Student’s t test. One-way ANOVA for multiple group comparisons, with Tukey’s post hoc test used for post hoc analyses.

Journal: Science Advances

Article Title: Sleep deprivation impairs follicular development attributable to granulosa cell pyroptosis mediated by S100A8/A9-driven macrophage M1 polarization

doi: 10.1126/sciadv.ady4767

Figure Lengend Snippet: ( A ) UMAP plot showing six distinct cell types in ovarian tissue. ( B ) Comparison of pyroptosis-related gene scores among different cell types in ovarian tissue ( n = 3). ( C ) Distribution of S100A8 and S100A9 gene expression in immune cells of ovarian tissue ( n = 3). ( D ) Comparison of S100a8 and S100a9 gene expression in immune cells of ovarian tissue between two groups of mice ( n = 3). ( E ) Measurement of S100A8/A9 levels in peripheral blood serum using ELISA ( n = 3). ( F ) Measurement of S100A8/A9 levels in ovarian tissue using ELISA ( n = 3). ( G ) Comparison of macrophage M1 polarization–related gene set scores in ovarian tissue between the two groups of mice. Larger dots represent a higher number of differential genes, and darker colors indicate larger gene expression differences. Data are presented as mean ± SEM. *** P < 0.001. The P value was calculated using a one-tailed unpaired Student’s t test. One-way ANOVA for multiple group comparisons, with Tukey’s post hoc test used for post hoc analyses.

Article Snippet: After observing the normal wall attachment of the cells, S100A8/A9 recombinant protein (5 μg/ml; R&D Systems) was added, and the cells were cultured in the incubator for 24 hours.

Techniques: Comparison, Gene Expression, Enzyme-linked Immunosorbent Assay, One-tailed Test

SD enhances neutrophil-driven S100A8/A9 release, activating the TLR4/MyD88/NF-κB signaling pathway, and promoting macrophage M1 polarization. This cascade amplifies ovarian inflammation and induces granulosa cell pyroptosis, ultimately compromising follicular development and embryonic quality in female mice.

Journal: Science Advances

Article Title: Sleep deprivation impairs follicular development attributable to granulosa cell pyroptosis mediated by S100A8/A9-driven macrophage M1 polarization

doi: 10.1126/sciadv.ady4767

Figure Lengend Snippet: SD enhances neutrophil-driven S100A8/A9 release, activating the TLR4/MyD88/NF-κB signaling pathway, and promoting macrophage M1 polarization. This cascade amplifies ovarian inflammation and induces granulosa cell pyroptosis, ultimately compromising follicular development and embryonic quality in female mice.

Article Snippet: After observing the normal wall attachment of the cells, S100A8/A9 recombinant protein (5 μg/ml; R&D Systems) was added, and the cells were cultured in the incubator for 24 hours.

Techniques:

CEBPB transcriptionally activates S100A8 to promote M2 macrophage polarization. A , Venn diagram of three datasets identifying four common secretory factors: differentially expressed genes from CEBPB-overexpressing 4T07 cells bulk RNA-seq, CEBPB binding peaks in 4T07 cells from anti-CEBPB ChIP-seq, and the profile of mouse secretory proteins. B , CEBPB binding peaks for S100A8 and Cxcl1 from anti-CEBPB ChIP-seq in 4T07 cells. C , ChIP-qPCR showing the CEBPB binding regions in the S100a8 promoter or upstream regions of Cxcl1 of 4T07 and EMT6 cells. D , Relative luciferase activity in CEBPB-overexpressing HEK293T cells with the S100a8 promoter WT or MUT, respectively. E-F , mRNA expression of S100a8 or Cxcl1 in 4T07 cells with CEBPB overexpression (E) or knockdown (F). G , Protein expression of S100A8 in 4T07 cells with or without CEBPB overexpression. H , Representative multiplex immunohistochemistry images of Ki67, CEBPB, and S100A8 staining in a TMA of TNBC patient tumor smaples ( n = 80). I , mRNA expression of S100a8 in eGFP -/lo and eGFP +/hi tumor cells sorted from 4T07-TetOn or EMT6-TetOn tumor masses. J-K , Percentages of CD206 (J) and MHCII (K) expressing BMDMs with the addition of rS100A8 (100 or 500 ng/mL) or rCXCL1 (100 or 500 ng/mL) for 48 hours. L-N , Tumor volume (L), image (M) and weight (N) of CEBPB-overexpressing with or without S100A8 knockdown EMT6 allografts in BALB/c mice ( n = 5). O-R , Flow cytometry analysis of CD8 + T cells (O), CD11b + F4/80 + cells (P), CD206 + macrophages (Q), and MHCII + macrophages (R) infiltrated in tumors developed in (L). n.s., not significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001.

Journal: Theranostics

Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming

doi: 10.7150/thno.124789

Figure Lengend Snippet: CEBPB transcriptionally activates S100A8 to promote M2 macrophage polarization. A , Venn diagram of three datasets identifying four common secretory factors: differentially expressed genes from CEBPB-overexpressing 4T07 cells bulk RNA-seq, CEBPB binding peaks in 4T07 cells from anti-CEBPB ChIP-seq, and the profile of mouse secretory proteins. B , CEBPB binding peaks for S100A8 and Cxcl1 from anti-CEBPB ChIP-seq in 4T07 cells. C , ChIP-qPCR showing the CEBPB binding regions in the S100a8 promoter or upstream regions of Cxcl1 of 4T07 and EMT6 cells. D , Relative luciferase activity in CEBPB-overexpressing HEK293T cells with the S100a8 promoter WT or MUT, respectively. E-F , mRNA expression of S100a8 or Cxcl1 in 4T07 cells with CEBPB overexpression (E) or knockdown (F). G , Protein expression of S100A8 in 4T07 cells with or without CEBPB overexpression. H , Representative multiplex immunohistochemistry images of Ki67, CEBPB, and S100A8 staining in a TMA of TNBC patient tumor smaples ( n = 80). I , mRNA expression of S100a8 in eGFP -/lo and eGFP +/hi tumor cells sorted from 4T07-TetOn or EMT6-TetOn tumor masses. J-K , Percentages of CD206 (J) and MHCII (K) expressing BMDMs with the addition of rS100A8 (100 or 500 ng/mL) or rCXCL1 (100 or 500 ng/mL) for 48 hours. L-N , Tumor volume (L), image (M) and weight (N) of CEBPB-overexpressing with or without S100A8 knockdown EMT6 allografts in BALB/c mice ( n = 5). O-R , Flow cytometry analysis of CD8 + T cells (O), CD11b + F4/80 + cells (P), CD206 + macrophages (Q), and MHCII + macrophages (R) infiltrated in tumors developed in (L). n.s., not significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001.

Article Snippet: BMDMs or PBMC-M φ cells were co-cultured in conditional medium supplemented with 10% FBS and 1% penicillin-streptomycin, or in complete DMEM or 1640 medium supplemented with rS100A8 (MCE, #HY- P73671 for BMDMs and #HY- P70531 for PBMC-M φ ) or rCXCL1 (MCE, #HY-P7188 for BMDMs) for 48 hours.

Techniques: RNA Sequencing, Binding Assay, ChIP-sequencing, ChIP-qPCR, Luciferase, Activity Assay, Expressing, Over Expression, Knockdown, Multiplex Assay, Immunohistochemistry, Staining, Flow Cytometry

The S100A8 inhibitor remodels TME and improves ICB efficacy. A-B , Percentages of CD206 expressing in BMDMs (A) or PBMC-Mφ (B) with the addition of paquinimod (PAQ, 5 µg/mL) and/or rS100A8 (500 ng/mL) for 48 hours. C , Migration assay of BMDMs in DMEM with the addition of PAQ (5 µg/mL) and/or rS100A8 (500 ng/mL) for 24 hours. D-E , Percentages of CD206 expressing in BMDMs (D) or PBMC-Mφ (E) co-cultured with CM and/or PAQ (5 µg/mL) for 48 hours, including CM from 4T07 (D) or MDA-MB-231 (E) with or without CEBPB overexpression. F-H , Tumor volume (F), image (G) and weight (H) of EMT6 allografts in BALB/c mice ( n = 5). Tumor-bearing mice were randomly divided into four groups until tumor volume reached approximately 100 mm 3 . I-L , Flow cytometry analysis of CD8 + T cells (I), CD11b + F4/80 + macrophages (J), CD206 + macrophages (K) MHCII + macrophages (L) infiltrated in tumors developed of the indicated groups. M , Kaplan-Meier analysis of S100A8 expression in a public cohort with 238 patients receiving anti-PD-1 therapy. n.s., not significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001 .

Journal: Theranostics

Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming

doi: 10.7150/thno.124789

Figure Lengend Snippet: The S100A8 inhibitor remodels TME and improves ICB efficacy. A-B , Percentages of CD206 expressing in BMDMs (A) or PBMC-Mφ (B) with the addition of paquinimod (PAQ, 5 µg/mL) and/or rS100A8 (500 ng/mL) for 48 hours. C , Migration assay of BMDMs in DMEM with the addition of PAQ (5 µg/mL) and/or rS100A8 (500 ng/mL) for 24 hours. D-E , Percentages of CD206 expressing in BMDMs (D) or PBMC-Mφ (E) co-cultured with CM and/or PAQ (5 µg/mL) for 48 hours, including CM from 4T07 (D) or MDA-MB-231 (E) with or without CEBPB overexpression. F-H , Tumor volume (F), image (G) and weight (H) of EMT6 allografts in BALB/c mice ( n = 5). Tumor-bearing mice were randomly divided into four groups until tumor volume reached approximately 100 mm 3 . I-L , Flow cytometry analysis of CD8 + T cells (I), CD11b + F4/80 + macrophages (J), CD206 + macrophages (K) MHCII + macrophages (L) infiltrated in tumors developed of the indicated groups. M , Kaplan-Meier analysis of S100A8 expression in a public cohort with 238 patients receiving anti-PD-1 therapy. n.s., not significant, *, P < 0.05, **, P < 0.01, ***, P < 0.001 .

Article Snippet: BMDMs or PBMC-M φ cells were co-cultured in conditional medium supplemented with 10% FBS and 1% penicillin-streptomycin, or in complete DMEM or 1640 medium supplemented with rS100A8 (MCE, #HY- P73671 for BMDMs and #HY- P70531 for PBMC-M φ ) or rCXCL1 (MCE, #HY-P7188 for BMDMs) for 48 hours.

Techniques: Expressing, Migration, Cell Culture, Over Expression, Flow Cytometry