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Diagramma
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GenScript corporation
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Becton Dickinson
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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Molecular mechanism of quorum sensing inhibition in Streptococcus by the phage protein paratox
doi: 10.1016/j.jbc.2021.100992
Figure Lengend Snippet: Prx forms stable complexes with various ComR orthologs. A , pull-down assay of Prx with various ComR orthologs including Type II ComR proteins (ComR S. mutans , ComR MGAS5005) Type I (ComR S. thermophilus ) and Type III (ComR S. suis ). Prx (with a His-tag) was first added to Ni-NTA resin to be used as bait, followed by incubation with purified ComR (no His-tag). Controls with beads without Prx (no Prx Controls) and purified protein (ComR controls) are shown. B , size-exclusion chromatography (SEC) binding assay with the ComR structural relative Rgg3. The top panel shows the chromatogram and the bottom panel a Coomassie-stained SDS-PAGE gel of the corresponding fractions.
Article Snippet: The expression construct for
Techniques: Pull Down Assay, Incubation, Purification, Size-exclusion Chromatography, Binding Assay, Staining, SDS Page