Journal: BMC Genomics

Article Title: Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection

doi: 10.1186/s12864-017-3562-4

Figure Lengend Snippet: Heatmap of the differentially expressed miRNAs. Heatmap showing log 2 -fold changes in the differentially expressed miRNAs at 1, 3, 5, 7, and 9 days post-infection when compared with the control

Article Snippet: Then, the cDNAs were used as the template for qPCR with the miRcute Plus miRNA qPCR Detection Kit (Tiangen, China).

Techniques: Infection, Control

Journal: BMC Genomics

Article Title: Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection

doi: 10.1186/s12864-017-3562-4

Figure Lengend Snippet: Schematic diagram of the interaction between the differentially expressed miRNAs and target genes. PITA and miRanda software were used to predict the target genes of the 36 differentially expressed miRNAs. The results show the intersection obtained by the software

Article Snippet: Then, the cDNAs were used as the template for qPCR with the miRcute Plus miRNA qPCR Detection Kit (Tiangen, China).

Techniques: Software

Journal: BMC Genomics

Article Title: Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection

doi: 10.1186/s12864-017-3562-4

Figure Lengend Snippet: Expression patterns of 10 representative target genes. Ten representative target genes involved in “complement and coagulation cascades” were selected for qPCR to examine the expression patterns at the six time points. The relative expression levels of the target genes at different time points were calculated as the ratio of gene expression level relative to that at 0 days (control) post-infection. All data represent the mean ± standard deviation values of three replicates. Significant differences ( P < 0.05) between the infected samples and control (0 day) are indicated with an asterisk (*)

Article Snippet: Then, the cDNAs were used as the template for qPCR with the miRcute Plus miRNA qPCR Detection Kit (Tiangen, China).

Techniques: Expressing, Coagulation, Gene Expression, Control, Infection, Standard Deviation

Journal: BMC Genomics

Article Title: Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection

doi: 10.1186/s12864-017-3562-4

Figure Lengend Snippet: Confirmation of the RNA-seq data by using RT-qPCR. Five known and five novel miRNAs were randomly selected for RT-qPCR analysis and compared with the data obtained using RNA-seq. The relative expression levels of the miRNAs at different time points were calculated as the ratio of gene expression level (qPCR) or normalized TPM (RNA-seq) relative to that at 0 days (control) post-infection. The data are represented as mean ± standard deviation of three replicates for the qPCR analysis

Article Snippet: Then, the cDNAs were used as the template for qPCR with the miRcute Plus miRNA qPCR Detection Kit (Tiangen, China).

Techniques: RNA Sequencing, Quantitative RT-PCR, Expressing, Gene Expression, Control, Infection, Standard Deviation