Journal: Anatolian Journal of Cardiology

Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

doi: 10.5152/AnatolJCardiol.2022.1223

Figure Lengend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).

Article Snippet: ID: Mm00465877_m1, Mm01201431_m1, Mm00446971_m1, and Mm01197698_m1).

Techniques: Gene Expression, Comparison

Journal: Anatolian Journal of Cardiology

Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

doi: 10.5152/AnatolJCardiol.2022.1223

Figure Lengend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a protein expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a protein expression. n = 6. No statistically significant comparison.

Article Snippet: ID: Mm00465877_m1, Mm01201431_m1, Mm00446971_m1, and Mm01197698_m1).

Techniques: Expressing, Comparison

Journal: Cancer Cell International

Article Title: Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line

doi: 10.1186/1475-2867-13-76

Figure Lengend Snippet: Assessment of ER calcium channels and pumps with EGF-induced EMT. Gene expression of A) SERCA1, B) SERCA2, C) SERCA3, D) IP 3 R1, (E) IP 3 R2, (F) IP 3 R3, and (G) RYR2 was assessed using real-time RT-PCR. Bar graphs show mean ± S.D. for nine individual wells from three independent experiments. * P < 0.05, unpaired t -test.

Article Snippet: The following Gene Expression Assays (Applied Biosystems) were used in this study: IP 3 R1 (Hs00181881_m1), IP 3 R2 (Hs00181916_m1), IP 3 R3 (Hs01573555_m1), MCU (Hs00293548_m1), NCLX (Hs00227951_m1), RYR1 (Hs00166991_m1), RYR2 (Hs00892840_m1), RYR3 (Hs00168821_m1), SERCA1 (Hs01092295_m1), SERCA2 (Hs00544877_m1), SERCA3 (Hs00193090_m1), SPCA1 (Hs00205122_m1), SPCA2 (Hs00208296_m1), Twist (Hs00361186_m1) and Vimentin (Hs00185584_m1).

Techniques: Gene Expression, Quantitative RT-PCR

Journal: Cardiovascular engineering and technology

Article Title: The ryanodine receptor contributes to the lysophosphatidylcholine-induced mineralization in valvular interstitial cells.

doi: 10.1007/s13239-020-00463-1

Figure Lengend Snippet: TaqMan Probe Accession Information

Article Snippet: RyR2 , Ss03374536_m1.

Techniques:

Journal: Cardiovascular engineering and technology

Article Title: The ryanodine receptor contributes to the lysophosphatidylcholine-induced mineralization in valvular interstitial cells.

doi: 10.1007/s13239-020-00463-1

Figure Lengend Snippet: (A) Immunocytochemistry of paVICs stained for the RyR (left) and no primary antibody control (right); scale = 100 μm. The protein is localized to the cytoplasm and is enriched in perinuclear vesicles that likely represent the endoplasmic reticulum. (B) qRT-PCR results mRNA expression of the three RyR isoforms by paVICs. Expression level is shown relative to GAPDH, and the grey dashed line represents the expression level of RyR1 in porcine skeletal muscle (positive control). Two biological replicates were used for all experiments; 3 technical replicates were used for IF staining, and 4–10 technical replicates were used for qRT-PCR.

Article Snippet: RyR2 , Ss03374536_m1.

Techniques: Immunocytochemistry, Staining, Control, Quantitative RT-PCR, Expressing, Positive Control

Journal: Archives of Endocrinology and Metabolism

Article Title: Thyroxine increases Serca2 and Ryr2 gene expression in heart failure rats with euthyroid sick syndrome

doi: 10.1590/2359-3997000000208

Figure Lengend Snippet: Gene expression of Serca2 and Ryr2 decreases in HF, while an increase is seen upon administration of T4.

Article Snippet: Real-time PCR (RT-qPCR) was used to quantitatively measure the messenger RNA (mRNA) levels of Serca2a (Rn00568762_m1) and Ryr2 (Rn01470303_m1) using TaqMan Universal PCR Master Mix (Applied Biosystems, CA, USA) and the Applied Biosystems StepOne Plus detection system.

Techniques: Gene Expression